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Volumn 271, Issue 5254, 1996, Pages 1413-1416

Uncoupling of GTP binding from target stimulation by a single mutation in the transducin α subunit

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CYCLIC GMP PHOSPHODIESTERASE; GLUTAMIC ACID; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; MUTANT PROTEIN; PROTEIN SUBUNIT; RHODOPSIN; TRANSDUCIN;

EID: 0029923266     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5254.1413     Document Type: Article
Times cited : (19)

References (22)
  • 7
    • 13344273073 scopus 로고    scopus 로고
    • note
    • TE203A.
  • 13
    • 13344252353 scopus 로고    scopus 로고
    • unpublished results
    • R. Mittal et al , unpublished results.
    • Mittal, R.1
  • 17
    • 13344290864 scopus 로고    scopus 로고
    • note
    • T in the absence of rhodopsin-containing lipid vesicles.
  • 19
    • 13344274621 scopus 로고    scopus 로고
    • note
    • T-GDP.
  • 20
    • 13344288333 scopus 로고    scopus 로고
    • note
    • 2, 1 mM DTT, 50 μM GTP, and 0.5 mM adenosine triphosphate. The reactions were incubated for 1 hour at room temperature and then Laemmli sample buffer was added and the samples subjected to SDS-polyacrylamide gel electrophoresis (PAGE) and autoradiography
  • 21
    • 13344254692 scopus 로고    scopus 로고
    • note
    • 35S]GTP-γ-S binding (12), were added to the PDE assays The PDE assays were initiated by the addition of cGMP to a final concentration of 5 mM, and the change in pH (in millivolts) was measured once per second. At the end of the assay period (∼200 s), the buffering capacity (millivolts per nanomole) was determined by the addition of 0 1 μmol of KOH to the reaction mixture The rate of hydrolysis of cGMP (nanomoles per second) was determined from the ratio of the slope of the pH record (millivolts per second) and the buffering capacily of the assay buffer (millivolts per nanomole).
  • 22
    • 13344296088 scopus 로고    scopus 로고
    • note
    • We thank J. V. Platko for discussions and molecular biological advice and C. Westmiller for technical assistance. Supported by grants from NIH


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.