Evidence for a role of calmodulin in calcium-induced noradrenaline release from permeated synaptosomes: Effects of calmodulin antibodies and antagonists

Journal of Neurochemistry

Volumn 66, Issue 5, 1996, Pages 1933-1942

  Hens, Jacques J H ^a   Oestreicher, A Beate ^a   De Wit, Marina ^a   Marquart, Arnoud ^a   Gispen, Willem Hendrik ^a   De Graan, Pierre N E ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

B 50 GAP 43; Calcium; Calmodulin; Calmodulin antagonists; Exocytosis; Permeated synaptosomes

Indexed keywords

CALMIDAZOLIUM; CALMODULIN; CALMODULIN BINDING PROTEIN; N (6 AMINOHEXYL) 5 CHLORO 1 NAPHTHALENESULFONAMIDE; NEUROMODULIN; NORADRENALIN; POLYMYXIN B; TRIFLUOPERAZINE;

ANIMAL TISSUE; ARTICLE; AUTOPHOSPHORYLATION; ENZYME ACTIVITY; MALE; NEUROTRANSMITTER RELEASE; NONHUMAN; NORADRENALIN RELEASE; PRIORITY JOURNAL; RAT; SYNAPTOSOME;

EID: 0029922172     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.66051933.x     Document Type: Article

References (59)

1. Ahnert-Hilger G., Bader M.-F., Bhakdi S., and Gratzl M. (1989) Introduction of macromolecules into bovine adrenal medullary chromaffin cells and rat pheochromocytoma cells (PC12) by permeabilization with streptolysin-O: inhibitory effect of tetanus toxin on catecholamine secretion. J. Neurochem. 52, 1751-1758.
2. Alexander K. A , Cimler B. M., Meier K. E., and Storm D. R. (1987) Regulation of calmodulin binding to P-57. J. Biol. Chem. 262, 6108-6113.
3. Andreasen T. J., Luetje C. W., Heideman W., and Storm D. R (1983) Purification of a novel calmodulin binding protein from bovine cerebral cortex membranes. Biochemistry 22, 4615-4618.
4. Augustine G. J., Charlton M. P., and Smith S. J. (1987) Calcium action in synaptic transmitter release. Annu. Rev. Neurosci. 10, 633-693.
5. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
6. 2+ sensor on the synaptic vesicle surface. Science 256, 1021-1023.
7. Buelt M. K., Glidden B. J., and Storm D. R. (1994) Regulation of p68 RNA helicase by calmodulin and protein kinase C. J. Biol. Chem. 269, 29367-29370.
8. Chapman E. R., Au D., Alexander K. A., Nicolson T. A., and Storm D. R. (1991) Characterization of the calmodulin binding domain of neuromodulin. J. Biol. Chem. 266, 207-213.
9. Clapham D. E. and Neher E. (1984) Trifluoperazine reduces inward ionic currents and secretion by separate mechanisms in bovine chromaffin cells. J. Physiol. (Lond.) 353, 541-564.
10. Colbran R. J. and Soderling T. R. (1990) Calcium/calmodulin-dependent protein kinase II. Curr. Top. Cell. Regul. 31, 181-221.
11. De Graan P. N. E. and Gispen W. H. (1993) The role of B-50/GAP-43 in transmitter release: studies with permeated synaptosomes Biochem. Soc. Trans. 21, 406-410.
12. De Graan P. N. E., Dekker L. V., Oestreicher A. B., Van der Voorn L., and Gispen W. H. (1989) Determination of changes in the phosphorylation state of the neuron-specific protein kinase C substrate B-50 (GAP43) by quantitative immunoprecipitation. J. Neurochem. 52, 17-23.
13. De Graan P. N. E., Oestreicher A. B., De Wit M., Kroef M., Schrama L. H., and Gispen W. H. (1990) Evidence for the binding of calmodulin to endogenous B-50 (GAP-43) in native synaptosomal plasma membranes. J. Neurochem. 55, 2139-2141.
14. 2+-induced catecholamine release. Soc. Neurosci. Abstr. 18, 634.
15. De Graan P. N. E., Moritz A., De Wit M., and Gispen W. H. (1993) Purification of B-50 by 2-mercaptoethanol extraction from rat brain synaptosomal plasma membranes. Neurochem. Res. 18, 875-881.
16. Dekker L. V., De Graan P. N. E., Oestreicher A. B., Versteeg D. H. G., and Gispen W. H. (1989) Inhibition of noradrenaline release by antibodies to B-50 (GAP-43). Nature 342, 74-76.
17. 3H-noradrenaline release in rat brain synaptosomes. Eur. J. Pharmacol. 188, 113-122.
18. Dekker L. V., De Graan P. N. E., Pijnappel P., Oestreicher A. B., and Gispen W. H. (1991) Noradrenaline release from streptolysin O-permeated rat cortical synaptosomes: effects of calcium, phorbol esters, protein kinase inhibitors, and antibodies to the neuron-specific protein kinase C substrate B-50 (GAP-43). J. Neurochem. 56, 1146-1153.
19. 1 fraction: homogeneity and morphology of subcellular fractions. Brain Res. 441, 59-71.
20. 2+ is absent. J. Biol. Chem. 270, 6741-6750.
21. 4. J. Immunol. 131, 821-825.
22. Greengard P., Valtorta F., Czernik A. J., and Benfenati F. (1993) Synaptic vesicle phosphoproteins and regulation of synaptic function. Science 259, 780-785.
23. Hegemann L., Van Rooijen L. A. A., Traber J., and Schmidt B. H. (1991) Polymyxin B is a selective and potent antagonist of calmodulin. Eur. J. Pharmacol. 207, 17-22.
24. 2+ trigger. J. Neurochem. 60, 1264-1273.
25. 2+-induced neuropeptide CCK-8 release from permeated synaptosomes. J. Neurochem. 61, 602-609.
26. 2+-induced noradrenaline release, B-50 phosphorylation and dephosphorylation, and calmodulin binding. J. Neurochem. 64, 1127-1136.
27. Hidaka H., Asano M., and Tanaka T. (1981) Activity-structure relationship of calmodulin antagonists: naphthalenesulfonamide derivatives. Mol. Pharmacol. 20, 571-578.
28. Ivins K. J., Neve K. A., Feller D. J., Fidel S. A., and Neve R. L. (1993) Antisense GAP-43 inhibits the evoked release of dopamine from PC12 cells. J. Neurochem. 60, 626-633.
29. Kenigsberg R. L. and Trifaró J. M. (1985) Micro-injection of calmodulin antibodies into cultured chromaffin cells block catecholamine release in response to stimulation. Neuroscience 14, 335-347.
30. Kenigsberg R. L., Côté A., and Trifaró J. M. (1982) Trifluoperazine, a calmodulin inhibitor, blocks secretion in cultured chromaffin cells at a step distal from calcium entry. Neuroscience 7, 2277-2281.
31. 2+ and target proteins, in Calmodulin (Cohen P. and Klee C. B., eds), pp. 35-56. Elsevier, Amsterdam.
32. Knight D. E. and Baker P. F. (1982) Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields. J. Membr. Biol. 68, 107-140.
33. Kristjansson G. I., Zwiers H., Oestreicher A. B., and Gispen W. H. (1982) Evidence that the synaptic phosphoprotein B-50 is localized exclusively in nerve terminals. J. Neurochem. 39, 371-378.
34. Liu Y. and Storm D. R. (1989) Dephosphorylation of neuromodulin by calcineurin. J. Biol. Chem. 264, 12800-12804.
35. Liu Y. and Storm D. R. (1990) Regulation of free calmodulin levels by neuromodulin: neuron growth and regeneration. Trends Pharmacol. Sci. 11, 107-111.
36. Llinás R., McGuinness T. L., Leonard C. S., Sugimori M., and Greengard P. (1985) Intraterminal injection of synapsin I or calcium/calmodulin-dependent protein kinase II alters neurotransmitter release at the squid giant synapse. Proc. Natl. Acad. Sci. USA 82, 3035-3039.
37. McMaster D., Zwiers H., and Lederis K. (1988) The growth-associated neuronal phosphoprotein B-50: improved purification, partial primary structure and characterization and localisation of proteolysis products. Brain Res. Bull. 21, 265-276.
38. Merril C. R., Goldman D., Sedman S. A., and Ebert M. H. (1981) Ultrasensitive stain for proteins in polyacrylamide gels shows regional variations in cerebrospinal fluid protein. Science 211, 1437-1438.
39. Momayezi M., Lumpert C. J., Kersken H., Gras U., Plattner H., Krinks M. H., and Klee C. B. (1987) Exocytosis induction of Paramecium tetraurelia cells by exogenous phosphoprotein phosphatase in vivo and in vitro: possible involvement of calcineurin in exocytotic membrane fusion. J. Cell Biol. 105, 181-189.
40. Morgan A., Roth D., Martin H., Aitken A., and Burgoyne R. D. (1993) Identification of cytosolic protein regulators of exocytosis. Biochem. Soc. Trans. 21, 401-405.
41. Nichols R. A., Sihra T. S., Czernik A. J., Nairn A. C., and Greengard P. (1990) Calcium/calmodulin-dependent protein kinase II increases glutamate and noradrenaline release from synaptosomes. Nature 343, 647-651.
42. Norden J. J., Lettes A., Costello B., Lin L., Wouters B., Bock S., and Freeman J. A. (1991) Possible role of GAP-43 in calcium regulation/neurotransmitter release. Ann. NY Acad. Sci. 627, 75-93.
43. Oestreicher A. B., Van Dongen C. J., Zwiers H., and Gispen W. H. (1983) Affinity-purified anti-B-50 protein antibody: interference with the function of the phosphoprotein B-50 in synaptic plasma membranes. J. Neurochem. 41, 331-340.
44. O'Neil K. T. and DeGrado W. F. (1990) How calmodulin binds its targets: sequence independent recognition of amphiphilic α-helices. Trends Biochem. Sci. 15, 59-64.
45. Pallen C. J. and Wang J. H. (1984) Regulation of calcineurin by metal ion. J. Biol. Chem. 259, 6134-6141.
46. Perrin D., Langley O. K., and Aunis D. (1987) Anti-α-fodrin inhibits secretion from permeabilized chromaffin cells. Nature 326, 498-501.
47. Rosahl T. W., Geppert M., Spillane D., Herz J., Hammer R. E., Malenka R. C., and Südhof T. C. (1993) Short-term synaptic plasticity is altered in mice lacking synapsin I. Cell 75, 661-670.
48. Sarafian T., Aunis D., and Bader M.-F. (1987) Loss of proteins from digitonin-permeabilized adrenal chromaffin cells essential for exocytosis. J. Biol. Chem 262, 16671-16676.
49. 2+ uptake in cultured adrenal chromaffin cells. Life Sci. 33, 2017-2024.
50. Schatzman R. C., Raynor R. L., and Kuo J. F. (1983) N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin antagonist, also inhibits phospholipid-sensitive calcium-dependent protein kinase. Biochim. Biophys. Acta 755, 144-147.
51. Schrama L. H., Heemskerk F. M. J., and De Graan P. N. E. (1989) Dephosphorylation of protein kinase C-phosphorylated B-50/ GAP-43 by the calmodulin-dependent phosphatase calcineurin. Neurosci. Res. Commun. 5, 141-147.
52. + depolarization in synaptosomes. J. Neurochem. 61, 443-450.
53. Spencer S. A. and Willard M. B. (1992) Does GAP-43 support axon growth by increasing the axonal transport velocity of calmodulin? Exp. Neurol. 115, 167-172.
54. Strittmatter S. M., Vartanian T., and Fishman M. C. (1992) GAP-43 as a plasticity protein in neuronal form and repair. J. Neurobiol. 23, 507-520.
55. Tanaka T. and Hidaka H. (1980) Hydrophobic regions function in calmodulin-enzyme(s) interactions. J. Biol. Chem. 255, 11078-11080.
56. Trifaró J.-M., Vitale M. L., and Rodríguez Del Castillo A. (1992) Cytoskeleton and molecular mechanisms in neurotransmitter release by neurosecretory cells. Eur. J. Pharmacol. 225, 83-104.
57. 2+-evoked catecholamine release. J. Neurochem. 59, 1717-1728.
58. Wylie D. C. and Vanaman T. C. (1988) Structure and evolution of the calmodulin family of calcium regulatory proteins, in Calmodulin (Cohen P. and Klee C. B., eds), pp. 1-15. Elsevier, Amsterdam.
59. Zwiers H., Verhaagen J., Van Dongen C. J., De Graan P. N. E., and Gispen W. H. (1985) Resolution of rat brain synaptic phosphoprotein B-50 into multiple forms by two-dimensional electrophoresis: evidence for multisite phosphorylation. J. Neurochem. 44, 1083-1090.