Expression of glycine receptor subunits in glial cells of the rat spinal cord

Journal of Neurochemistry

Volumn 66, Issue 4, 1996, Pages 1383-1390

  Kirchhoff, Frank ^b   Mülhardt, Cornel ^a   Pastor, Andrea ^b   Becker, Cord Michael ^a   Kettenmann, Helmut ^b  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Glial cells; Glycine receptor; Patch clamp; Single cell reverse transcription polymerase chain reaction; Spinal cord

Indexed keywords

COMPLEMENTARY DNA; GLYCINE RECEPTOR; MESSENGER RNA; OLIGONUCLEOTIDE; RECEPTOR SUBUNIT;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; GLIA CELL; NERVE CELL CULTURE; NEUROTRANSMISSION; NEWBORN; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; SPINAL CORD;

EID: 0029921383     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.66041383.x     Document Type: Article

References (37)

1. Adams R. H., Sato K., Shimada S., Tohyama M., Püschel A. W., and Betz H. (1995) Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents. J. Neurosci. 15, 2524-2532.
2. Aoki C., Venkatesan C., Go C. G., Mong J. A., and Dawson T. M. (1994) Cellular and subcellular localization of NMDA-R1 subunit immunoreactivity in the visual cortex of adult and neonatal rats. J. Neurosci. 14, 5202-5222.
3. Becker C.-M. (1992) Selective neurotoxicity: convulsants acting at the inhibitory glycine receptor, in Handbook of Experimental Pharmacology (Herken H. and Hucho F., eds), pp. 539-575. Springer, Heidelberg.
4. Becker C.-M. (1995) Glycine receptors: molecular heterogeneity and implications for disease. Neuroscientist 1, 130-141.
5. Becker C.-M., Hoch W., and Betz H. (1988) Glycine receptor heterogeneity in rat spinal cord during postnatal development. EMBO J. 7, 3717-3726.
6. Becker C.-M., Betz H., and Schröder H. (1993) Expression of inhibitory glycine receptors in postnatal rat cerebral cortex. Brain Res. 606, 220-226.
7. + buffer capacity and morphology of glial cells in the corpus callosum slice. J. Neurosci. 11, 3008-3024.
8. Betz H. (1992) Structure and function of inhibitory glycine receptors. Q. Rev. Biophys. 25, 381-394.
9. Cambier D. and Pessac B. (1987) Free amino acid content of astroglial cell clones derived from 8-day postnatal mouse cerebella J. Neurochem. 49, 802-805.
10. Chvátal A., Pastor A., Mauch M., Syková E., and Kettenmann H. (1995) Heterogeneity of identified glial cells in the rat spinal cord slice: cell morphology and ion channel properties. Eur. J. Neurosci. 7, 129-142.
11. Edwards F. A., Konnerth A., Sakmann B., and Takashaki T. (1989) A thin slice preparation for patch-clamp recordings from neurons of the mammalian central nervous system. Pflugers Arch. 414, 600-612.
12. Grenningloh G., Rienitz A., Schmitt B., Methfessel C., Zensen M., Beyreuther K., Gundelfinger E. D., and Betz H. (1987) The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors. Nature 328, 215-220.
13. Grenningloh G., Pribilla I , Prior P., Multhaup G., Beyreuther K., Taleb O., and Betz H. (1990) Cloning and expression of the 58 kd beta subunit of the inhibitory glycine receptor. Neuron 4, 963-970.
14. Hamill O. P., Marty E., Neher E., Sakmann B., and Sigworth F. J. (1981) Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391, 85-100.
15. Hoch W., Betz H., Schramm M., Wolters I., and Becker C.-M. (1992) Modulation by NMDA-receptor antagonists of glycine receptor isoform expression in cultured spinal cord neurons. Eur. J. Neurosci. 4, 389-395.
16. - efflux from cultured oligodendrocytes. Neurosci. Lett. 97, 334-339.
17. Kettenmann H. and Ransom B. R., eds (1995) Neuroglial Cells. Oxford University Press, New York.
18. Kettenmann H., Backus K.-H., and Schachner M. (1984a) Aspartate, glutamate and gamma-aminobutyric acid depolarize cultured astrocytes. Neurosci. Lett. 52, 25-29.
19. Kettenmann H., Gilbert P., and Schachner M. (1984b) Depolarization of cultured oligodendrocytes by glutamate and GABA. Neurosci. Lett. 47, 271-276.
20. - channels in cultured astrocytes. Brain Res. 404, 1-9.
21. 2+], increase in murine precursor cells of the oligodendrocyte lineage. Eur. J. Neurosci. 4, 1049-1058.
22. Kirchhoff F., Blümcke I., Ohlemeyer C., Celio M. R., and Kettenmann H. (1993) Patch-clamp analysis of glial cells in brain slices of murine neocortex. Soc. Neurosci. Abstr. 19, 686.
23. Kuhse J., Schmieden V., and Betz H. (1990) Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor. J. Biol. Chem. 265, 22317-22320.
24. Kuhse J., Kuryatov A., Maulet Y., Malosio M. L., Schmieden V., and Betz H. (1991) Alternative splicing generates two isoforms of the alpha 2 subunit of the inhibitory glycine receptor. FEBS Lett. 283, 73-77.
25. Lambolez B., Audinat E., Bochet P., Crepel F., and Rossier J. (1992) AMPA receptor subunits expressed by single Purkinje cells. Neuron 9, 247-258.
26. Malosio M. L., Marqueze-Pouey B., Kuhse J., and Betz H. (1991) Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain. EMBO J. 10, 2401-2409.
27. Matzenbach B., Maulet Y., Sefton L., Courtier B., Avner P., Guenet J. L., and Betz H. (1994) Structural analysis of mouse glycine receptor alpha subunit genes. Identification and chromosomal localization of a novel variant. J. Biol Chem. 269, 2607-2612
28. Mülhardt C., Fischer M., Gass P., Simon-Chazottes D., Gucnet J. L., Kuhse J., Betz H., and Becker C. M. (1994) The spastic mouse: aberrant splicing of glycine receptor beta subunit mRNA caused by intronic insertion of L1 element. Neuron 13, 1003-1015.
29. Müller T., Grosche J., Ohlemeyer C., and Kettenmann H. (1993) NMDA-activated currents in Bergmann glial cells. Neuroreport 4, 671-674.
30. Pastor A., Chvátal A., Syková E., and Kettenmann H. (1995) Glycine- and GABA-activated currents in identified glial cells of the developing rat spinal cord. Eur. J. Neurosci. 7, 1188-1198.
31. Pfeiffer F., Graham D., and Betz H. (1982) Purification by affinity chromatography of the glycine receptor of rat spinal cord. J. Biol. Chem. 257, 9389-9393.
32. Pribilla I., Takagi T., Langosch D., Bormann J., and Betz H. (1992) The atypical M2 segment of the beta subunit confers picrotoxinin resistance to inhibitory glycine receptor channels. EMBO J. 11, 4305-4311.
33. Sambrook J., Fritsch E. F., and Maniatis T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
34. Sato K., Yoshida S., Fujiwara K., Tada K., and Tohyama M. (1991) Glycine cleavage system in astrocytes. Brain Res. 567, 64-70.
35. Saul B., Schmieden V., Kling C., Mülhardt C., Gass P., Kuhse J., and Becker C.-M. (1994) Point mutation of glycine receptor alpha 1 subunit in the spasmodic mouse affects agonist responses. FEBS Lett. 350, 71-76.
36. Schmieden V., Grenningloh G., Schofield P. R., and Betz H. (1989) Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor. EMBO J. 8, 695-700.
37. Sontheimer H., Becker C.-M., Pritchett D., Schofield P., Grenningloh G., Kettenmann H., Betz H., and Seeburg P. (1989) Functional chloride channels by mammalian cell expression of rat glycine receptor subunit. Neuron 2, 1491-1497.