Trichloroacetic acid precipitation by ultracentrifugation to concentrate dilute protein in viscous solution

BioTechniques

Volumn 20, Issue 6, 1996, Pages 982-984

  Hwang, Byung Joon ^a   Chu, Gilbert ^a  

^a STANFORD UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRICHLOROACETIC ACID;

AMINO ACID SEQUENCE; ARTICLE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; ULTRACENTRIFUGATION;

BETA-GALACTOSIDASE; PRECIPITATION; PROTEINS; SERUM ALBUMIN, BOVINE; SOLUTIONS; TRICHLOROACETIC ACID; ULTRACENTRIFUGATION; VISCOSITY;

EID: 0029920123     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206bm09     Document Type: Article

References (4)

1. Bosserhoff, A., J. Wallach and R. Frank. 1989. Micropreparative separation of peptides derived from sodium dodecyl sulfate-solubilized proteins. J. Chromatogr. 473:71-17.
2. Hwang, B.J., A. Smith and G. Chu. 1996. Internal sequence analysis of proteins eluted from polyacrylamide gels. J. Chromatog. B. Biomed. Appl. (In press).
3. Mahuran, D., P. Clements, M. Carrella and P.M. Strasberg. 1983. A high recovery method for concentrating microgram quantities of protein from large volumes of solution. Anal. Biochem. 129:513-516.
4. Ozols, J. 1990. Amino acid analysis, p. 587-601. In M.P. Deutscher (Ed.), Methods in Enzymology. Vol. 182. Academic Press, New York.