The effect of neurobiotin on membrane properties and morphology of intracellularly labeled neurons

Journal of Neuroscience Methods

Volumn 65, Issue 1, 1996, Pages 27-32

  Xi, Xin Z ^a   Xu, Zao C ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Electrophysiology; In vivo; Intracellular recording; Intracellular staining; Morphology; Neurobiotin; Striatal neuron

Indexed keywords

BIOTIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; CORPUS STRIATUM; ELECTROPHYSIOLOGY; INTRACELLULAR RECORDING; IONTOPHORESIS; MALE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT; STAINING;

EID: 0029919847     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(95)00140-9     Document Type: Article

References (14)

1. Brown, A.G. and Fyffe, R.E.W. (1984) Intracellular Staining of Mammalian Neurons, Academic, London.
2. Cullheim, S. and Kellerth, J.O. (1976) Combined light and electron microscopic tracing of neurons, including axons and synaptic terminals after intracellular injection of horseradish peroxidase, Neurosci. Lett. 2: 307-313.
3. Hille, B. (1992) Potassium channels and chloride channels. In: B. Hille (Ed.), Ionic Channels of Excitable Membranes. 2nd edn., Sinauer, pp. 115-136.
4. Horikawa, K. and Armstrong, W.E. (1988) A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates. J. Neurosci. Methods, 25: 1-11.
5. Kita, H. and Armstrong, W. (1991) A biotin-containing compound N-(2-aminoethyl) biotinamide for intracellular labeling and neuronal tracing studies: comparison with biocytin, J. Neurosci. Methods, 37: 141-150.
6. Kitai, S.T., Kocsis, J.D., Preston, R.J. and Sugimori, M. (1976) Monosynaptic inputs in caudate neurons identified by intracellular injection of HRP, Brain Res., 109: 601-606.
7. Koester, J. (1990 Voltage-gated ion channels and the generation of the action potential. In: E.R. Kandel, J.H. Schwartz and T.M. Jessell (Eds.), Principles of Neural Science. 3rd edn., Appleton & Lange, pp. 104-118.
8. Nicholson, C. and Kater, S.B. (1973) The development of intracellular staining. In: S.B. Kater and C. Nicholson (Eds.), Intracellular Staining in Neurobiology, Springer, New York, pp. 1-19.
9. Stewart, W.W. (1978) Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell, 14: 741-759.
10. Stewart, W.W. (1981) Lucifer dyes: highly fluorescent dyes for biological tracing. Nature, 292: 17-21.
11. Tseng, G.F., Parada, I. and Prince, D.A. (1991) Double-labeling with rhodamine beads and biocytin: a technique for studying corticospinal and other projection neurons in vitro. J. Neurosci. Methods, 37 (1991) 121-131.
12. Wilson, C.J. (1993) The generation of natural firing patterns in neostriatal neurons. In: C.W. Arbuthnott and P.C. Emson (Eds.), Progress in Brain Research, Vol. 99, Elsevier, Amsterdam, pp. 277-297.
13. Xu, Z.C., Wilson, C.J. and Emson, P.C. (1991) Synaptic potentials evoked in spiny neurons in rat neostriatal grafts by cortical and thalamic stimulation. J. Neurophysiol., 65: 477-493.
14. Xu, Z.C. (1995) Neurophysiological changes of spiny neurons in rat neostriatum after transient forebrain ischemia: an in vivo intracellular recording and staining study. Neuroscience, 67: 823-836.