Heterogeneity of growth hormone (GH)-producing cells in aging male rats: Ultrastructure and GH gene expression in somatotrope subpopulations

Molecular and Cellular Endocrinology

Volumn 118, Issue 1-2, 1996, Pages 181-191

  Dobado Berrios, Pablo M ^a   Ruíz Navarro, Antonio ^a   Almadén, Yolanda ^a   Malagón, María M ^a   Garrido, José C ^a   Ramírez Gutiérrez, José L ^a   Gracia Navarro, Francisco ^a  

^a UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

Aging; Cellular heterogeneity; Growth hormone; mRNA; Somatotropes (rat); Ultrastructure

Indexed keywords

GROWTH HORMONE; MESSENGER RNA;

AGING; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL SEPARATION; CELL SUBPOPULATION; CELL ULTRASTRUCTURE; GENE EXPRESSION; GROWTH HORMONE RELEASE; HYPOPHYSIS CELL; IN SITU HYBRIDIZATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; ROUGH ENDOPLASMIC RETICULUM; SECRETORY GRANULE;

EID: 0029919680     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/0303-7207(96)03781-1     Document Type: Article

References (48)

1. Sonntag, W.E., Steger, R.W., Forman, L.J. and Meites, J. (1980) Decreased pulsatile release of growth hormone in old male rats. Endocrinology 107, 1875-1879.
2. Takahashi, S., Gottschall, P.E., Quigley, K.L., Goya, R.G. and Meites, J. (1987) Growth hormone secretory patterns in young, middle-aged and old female rats. Neuroendocrinology 46, 137-142.
3. Kahler, L., Gliessman, P., Craven, J., Hill, J. and Critchlow, V. (1986) Loss of enhanced nocturnal growth hormone secretion in aging rhesus monkeys. Endocrinology 119, 1281-1284.
4. Carlson, H., Gillin, J., Gorden, P. and Snyder, F. (1972) Absence of sleep-related growth hormone peaks in aged normal subjects and in acromegaly. J. Clin. Endocrinol. Metab. 34, 1102-1107.
5. Finkelstein, J.W., Roffwarg, H.P., Boyar, R.M., Kream, J. and Hellman, L. (1972) Age-related change in the twenty-four hour spontaneous secretion of growth hormone. J. Clin. Endocrinol. Metab. 35, 665-670.
6. Rudman, D., Kutner, M.H., Rogers, C.M., Lubin, M.F., Fleming, G.A. and Bain, R.P. (1981) Impaired growth hormone secretion in the adult population. J. Clin. Invest. 67, 1361-1369.
7. Prinz, P.N., Weitzmann, E.D., Cunningham, G.R. and Karacan, I. (1983) Plasma growth hormone during sleep in young and aged men. J. Gerontol 38, 519-524.
8. Ho, K.Y., Evans, W.S., Blizzard, R.M., Veldhuis, J.D., Merriam, G.R., Samojlik, E., Furlanetto, R., Rogol, A.D., Kaiser, D.L. and Thorner, M.O. (1987) Effects of sex and age on the 24-hour proile of growth hormone secretion in man: importance of endogenous estradiol concentrations. J. Clin. Endocrinol. Metab. 64, 51-58.
9. Sonntag, W.E. and Meites, J. (1988) Decline in growth hormone secretion in aging animals and man. In: Regulation of Neuroendocrine Aging (Everitt, A.V. and Walton, J.R., eds.), pp. 111-124, Vol. 24, Karger, Basel.
10. Sonntag, W.E., Forman, L.J., Miki, N., Steger, R.W., Ramos, T., Arimura, A. and Meites, J. (1981) Effect of CNS active drugs and somatostatin antiserum on growth hormone release in young and old male rats. Neuroendocrinology 33, 73-78.
11. Locatelli, V., Arimura, A., Torsello, A., Cella, S.G. and Müller, E.E. (1984) Somatostatin antiserum antagonizes the impaired ability of hpGRF-40 to stimulate growth hormone release in old unanesthetized male rats. Neuroendocrinol. Lett. 6, 261-265.
12. De Gennaro Colonna, V., Zoli, M., Cocchi, D., Maggi, A., Marrama, P., Agnati, L.F. and Müller, E.E. (1989) Reduced growth hormone releasing factor (GHRF)-like immunoreactivity and GHRF gene expression in the hypothalamus of aged rats. Peptides 10, 705-708.
13. De Gennaro Colonna, V., Cella, S.G., Parenti, M., Locatelli, V., Cocchi, D. and Müller E.E. (1994) Neuroendocrine aging: its impact on somatotrophic function. Neurochem. Int. 25, 5-10.
14. Müller, E.E., Cella, S.G., De Gennaro Colonna, V., Parenti, M., Cocchi, D. and Locatelli, V. (1993) Aspects of the neuroendocrine control of growth hormone secretion in ageing mammals. In: Neurobiology and Neuroendocrinology of Ageing (Falvo, R., Bartke, A., Giacobini, E. and Thorne, A., eds.), pp. 99-114, Vol. 46, JRF, Cambridge.
15. Ceda, G.P., Valenti, G., Butturini, U. and Hoffman, A.R. (1986) Diminished pituitary responsiveness to growth hormone-releasing factor in aging male rats. Endocrinology 118, 2109-2114.
16. Robberecht, P., Gillard, M., Waelbroeck, M., Camus, J.-C., De Neef, P. and Christophe, J. (1986) Decreased stimulation of adenylate cyclase by growth hormone-releasing factor in the anterior pituitary of old rats. Neuroendocrinology 44, 429-432.
17. Abribat, T., Deslauriers, N., Brazeau, P. and Gaudreau, P. (1991) Alterations of pituitary growth hormone-releasing factor binding sites in aging rats. Endocrinology 128, 633-635.
18. Deslauriers, N., Gaudreau, P., Abribat, T., Renier, G., Petitclerc, D. and Brazeau, P. (1991) Dynamic of growth hormone responsiveness to growth hormone releasing factor in aging rats: peripheral and central influences. Neuroendocrinology 53, 439-446.
19. Parenti, M., Cocchi, D., Ceresoli, G., Marcozzi, C. and Müller, E.E. (1991) Age-related changes of growth hormone secretory mechanisms in the rat pituitary gland. J. Endocrinol. 131, 251-257.
20. Ingram, D.C., Keefe, P.D., Wooding, F.P.B. and Bicknell, R.J. (1983) Morphological characterisation of lactotrophs separated from the bovine pituitary by rapid enrichment technique. Cell Tissue Res. 252, 655-659.
21. Velkeniers, B., Hooghe-Peters, E.L., Hooghe, R., Belayew, A., Smets, G., Claeys, A., Robberecht, P. and Vanhaelst, L. (1988) Prolactin cell subpopulations separated on discontinuous Percoll gradient: an immunocytochemical, biochemical and physiological characterization. Endocrinology 123, 1619-1630.
22. Kazemzadeh, M., Velkeniers, B., Herregodts, P., Collumbien, R., Finné, E., Derde, M.P., Vanhaelst, L. and Hooghe-Peters, E.L. (1992) Differential dopamine-induced prolactin mRNA levels in various prolactin-secreting cell (sub)populations. J. Endocrinol. 132, 401-409.
23. Torronteras, R., Castaño, J.P., Almaden, Y., Ruiz-Navarro, A. and Gracia-Navarro, F. (1993) Hormonal storage patterns and morphological heterogeneity of porcine gonadotrope cells during postnatal development. Mol. Cell. Endocrinol. 97, 51-59.
24. Gonzalez de Aguilar, J.E., Tonon, M.C., Ruiz-Navarro, A., Vaudry, H. and Gracia-Navarro, F. (1994) Morphological and functional heterogeneity of frog melanotrope cells. Neuroendocrinology 59, 176-182.
25. Snyder, G. and Hymer, W.C. (1975) A short method for the isolation of somatotrophs from the rat pituitary gland. Endocrinology 96, 792-796.
26. Snyder, G., Hymer, W.C. and Snyder, J. (1977) Functional heterogeneity in somatotrophs isolated from the rat anterior pituitary. Endocrinology 101, 788-799.
27. Hall, M.S., Howell, S.L., Schulster, D. and Wallis, M. (1982) A procedure for the purification of somatotrophs isolated from rat anterior pituitary glands Percoll density gradients. J. Endocrinol. 94, 257-266.
28. Torronteras, R., Castaño, J.P., Ruiz-Navarro, A. and Gracia-Navarro, F. (1993) Application of a Percoll density gradient to separate and enrich porcine pituitary cell types. J. Neuroendocrinol. 5, 257-266.
29. Hsu, S.M., Raine, L. and Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and labelled PAP procedures. J. Histochem. Cytochem. 29, 577-580.
30. Roth, J. (1983) The colloidal gold marker system for light and electron microscopic cytochemistry. In: Techniques in Immunocytochemistry (Bullok, G.R. and Petrusz, P., eds.), pp. 217-284, Vol. 2, Academic Press, London.
31. Bendayan, M. (1984) Protein A-gold electron microscopic immunocytochemistry: methods, applications, and limitations. J. Electron Micr. Tech. 1, 243-270.
32. Weibel, E.R. (1979) Stereological Methods. Vol. 1: Practical Methods for Biological Morphometry, pp. 1-253, Academic Press, London.
33. Malagón, M.M., Castaño, J.P., Dobado-Berrios, P.M., García-Navarro, S. and Gracia-Navarro, F. (1991) Human pancreatic growth hormone-releasing factor (1-44) stimulates GH cells in an anuran amphibian (Rana perezi). Gen. Comp. Endocrinol. 84, 461-469.
34. Weibel, E.R. and Gomez, D.M. (1962) A principle for counting tissue structures on random sections. J. Appl. Physiol. 17, 343-352.
35. Takahashi, S., Kawashima, S., Seo, H. and Matsui, N. (1990) Age-related changes in growth hormone and prolactin messenger RNA levels in the rat. Endocrinol. Jpn. 37, 827-840.
36. Takahashi, S. (1991) Immunocytochemical and immuno-electron-microscopical study of growth hormone cells in male and female rats of various ages. Cell Tissue Res. 266, 275-284.
37. Hymer, W.C., Evans, W.H., Kraicer, J., Mastro, A., Davis, J. and Griswold, E. (1973) Enrichment of cell types from the rat adenohypophysis by sedimentation at unit gravity. Endocrinology 92, 275-287.
38. Ohlsson, L., Lindström, P. and Norlund, R. (1988) An ultrastructural and functional characterization of rat somatotrophs highly enriched on a continuous Percoll density gradient. Mol. Cell. Endocrinol. 59, 47-55.
39. Hofland, L.J., van Koetsveld, P.M., Verleun, T.M. and Lamberts, S.W.J. (1990) Long-term culture of rat mammotrope and somatotrope subpopulations separated on continuous Percoll density gradients: effects of dopamine, TRH, GHRH and somatostatin. Acta Endocrinol. (Copenhagen) 122, 127-136.
40. García, J., Jiménez, L., García, P.P., Leal, A., Astorga, R., García, J. and Bernal, G. (1989) Histol. Histopathol. 4, 223-233.
41. Hassoun, J., Jaquet, P., Devictor, B., Andonian, C., Grisoli, F., Gunz, G. and Toga, M. (1985) Bromocriptine effects on cultured human prolactin-producing pituitary adenomas: in vitro ultrastructural, morphometric, and immunoelectron microscopic studies. J. Clin. Endocrinol. Metab. 61, 686-692.
42. Landolt, A.M., Osterwalder, V. and Landolt, T.A. (1987) Storage and release of secretory granules in human prolactinomas: modification by bromocriptine. J. Endocrinol. 113, 495-499.
43. Bäck, N. (1989) The effect of bromocriptine on the intermediate lobe of de rat pituitary: an electron-microscopic, morphometric study. Cell Tissue Res. 255, 405-410.
44. Hopkins, C.R. and Farquhar, M.G. (1973) Hormone secretion by cells dissociated from rat anterior pituitaries. J. Cell Biol. 59, 276-303.
45. Bäck, N. and Soinila, S. (1994) Regulation of secretory granule formation in chronically hypersecretory melanotrophs in the rat pituitary. Cell Tissue Res. 275, 339-344.
46. Martinoli, M.G., Ouellet, J., Rhéaume, E. and Pelletier, G. (1991) Growth hormone and somatostatin gene expression in adult and aging rats as measured by quantitative in situ hybridization. Neuroendocrinology 54, 607-615.
47. Sapolsky, R.M., Kery, L.C. and McEwen, B.S. (1986) The neuroendocrinology of stress and aging. The glucocorticoid cascade hypothesis. Endocr. Rev. 7, 284-301.
48. Lippicontt-Schwartz, J. (1993) Bidirectional membrane traffic between the endoplasmic reticulum and Golgi apparatus. Trends Cell Biol. 3, 81-88.