Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody

Neuroscience Research

Volumn 24, Issue 3, 1996, Pages 237-243

  Takagawa, Kiyoshi ^a,b   Salvaterra, Paul ^b  

^a TORANOMON HOSPITAL   (Japan)

^b CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

Antibodies, monoclonal; Blotting; Choline acetyltransferase; Drosophila melanogaster; Western

Indexed keywords

CHOLINE ACETYLTRANSFERASE; MONOCLONAL ANTIBODY;

ARTICLE; DROSOPHILA; ENZYME ANALYSIS; GENOTYPE; IMMUNOBLOTTING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; STRUCTURAL GENE; TEMPERATURE SENSITIVE MUTANT;

EID: 0029919666     PISSN: 01680102     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-0102(95)00999-X     Document Type: Article

References (23)

1. Batteiger, B., Newhall, V.W.J. and Jones, R.B. (1982) The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J. Immunol. Methods, 55: 297-307.
2. Driskell, W.J., Weber, B.H. and Roberts, E. (1978) Purification of choline acetyltransferase from Drosophila melanogaster. J. Neurochem., 30: 1135-1141.
3. Fonnum, F. (1975) A rapid radiochemical method for the determination of choline acetyltransferase. J. Neurochem., 24: 407-409.
4. Greenspan, R.J. (1980) Mutations of choline acetyltransferase and associated neural defects in Drosophila melanogaster. J. Comp. Physiol. [A]., 137: 83-92.
5. Habert, E., Birman, S. and Mallet, J. (1992) High-Level Synthesis and Fate of Acetylcholine in Baculovirus-Infected Cells: Characterization and Purification of Recombinant Rat Choline Acetyltransferase. J. Neurochem., 58: 1447-1453.
6. Hanahan, D. (1983) Studies on Transformation of Escherichia coli with plasmids. J. Mol. Biol., 166: 557-580.
7. Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, New York.
8. Itoh, N., Slemmon, J.R., Hawke, D.H., Williamson, R., Morita, E., Itakura, K., Roberts, E., Shively, J.E., Crawford, G.D. and Salvaterra, P.M. (1986) Cloning of Drosophila choline acetyltransferase cDNA. Proc. Natl. Acad. Sci. USA, 83: 4081-4085.
9. Johnson, D.A., Gautsch, J.W., Sportsman, J.R. and Elder, J.H. (1984) Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene. Anal. Tech., 1: 3-8.
10. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
11. Leary, J.J., Brigati, D.J. and Ward, D.C. (1983) Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: bioblots. Proc. Natl. Acad. Sci. USA, 80: 4045-4049.
12. McCaman, R.E., Carbini, L., Maines, V. and Salvaterra, P.M. (1988) Single RNA species injected in Xenopus oocyte directs the synthesis of active choline acetyltransferase. Brain Res. Mol. Brain Res., 3: 107-114.
13. Muñoz-Maines, V.J., Slemmon, J.R., Panicker, M.M., Neighbor, N. and Salvaterra, P.M. (1988) Production of polyclonal antisera to choline acetyltransferase using a fusion protein produced by a cDNA clone. J. Neurochem., 50: 167-175.
14. Salvaterra, P.M. and McCaman, R.E. (1985) Choline acetyltransferase and acetylcholine levels in Drosophila melanogaster: a study using two temperature-sensitive mutants. J. Neurosci., 5: 903-910.
15. Salvaterra, P.M. and Vaughn, J.E. (1989) Regulation of choline acetyltransferase. Int. Rev. Neurobiol., 31: 81-143.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
17. Slemmon, J.R., Campbell, G.A., Selski, D.J. and Bramson, H.N. (1991) The amino terminus of the putative Drosophila choline acetyltransferase precursor is cleaved to yield the 67 kDa enzyme. Brain Res. Mol. Brain Res., 9: 245-252.
18. Slemmon, J.R., Salvaterra, P.M., Crawford, G.D. and Roberts, E. (1982) Purification of choline acetyltransferase from Drosophila melanogaster. J. Biol. Chem., 257: 3847-3852.
19. Studier, F.W., Rosenberg, A.H., Dunn, J.J. and Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol., 185: 60-89.
20. Sugihara, H., Andrisani, V. and Salvaterra, P.M. (1990) Drosophila choline acetyltransferase uses a non-AUG initiation codon and full length RNA is inefficiently translated. J. Biol. Chem., 265: 21714-21719.
21. Tajima, Y. and Salvaterra, P.M. (1990) Sequence of choline acetyltransferase temperature-sensitive mutants determined by the polymerase chain reaction. Neuroscience, 39: 245-250.
22. Tajima, Y. and Salvaterra, P.M. (1992) Positive and negative feedback regulation of choline acetyltransferase mRNA levels in Drosophila: a study using temperature-sensitive mutants and embryo cell cultures. Brain Res. Mol. Brain Res., 13: 213-221.
23. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76: 4350-4354.