Genomic organization of M line titin and its tissue-specific expression in two distinct isoforms

Journal of Molecular Biology

Volumn 256, Issue 3, 1996, Pages 556-563

  Kolmerer, Bernhard ^a   Olivieri, Nicoletta ^a   Witt, Christian C ^b,d   Herrmann, Bernhard G ^c   Labeit, Siegfried ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b BIELEFELD UNIVERSITY   (Germany)

^c MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS   (Germany)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Differential splicing; Intron conservation; M line; Muscle ultrastructure; Titin (connectin)

Indexed keywords

CONNECTIN; ISOPROTEIN; MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; EMBRYO; EMBRYO DEVELOPMENT; EXON; GENETIC CONSERVATION; GENETIC ORGANIZATION; HUMAN; IN SITU HYBRIDIZATION; INTRON; MOUSE; NONHUMAN; PRIORITY JOURNAL; RABBIT; RNA SPLICING; SARCOMERE; SKELETAL MUSCLE; TISSUE SPECIFICITY; VERTEBRATE;

ANIMALIA; ORYCTOLAGUS CUNICULUS; VERTEBRATA;

EID: 0029919189     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0108     Document Type: Article

References (26)

1. Carlsson, E. & Thornell, L. E. (1987). Diversification of the myofibrillar M band in rat skeletal muscle during postnatal development. Cell Tissue Res. 248, 169-180.
2. Church, G. M. & Gilbert, W. (1984). Genomic sequencing. Proc. Natl Acad. Sci. USA, 81, 1991-1995.
3. Conlon, R. A. & Herrmann, B. G. (1993). Detection of messenger RNA by in situ hybridization to post-implantation embryo whole mounts. Methods Enzymol. 225, 373-383.
4. Craig, R. & Offer, G. (1976). The location of C protein in rabbit skeletal muscle. Proc. Roy. Soc. ser. B, 192, 325-332.
5. Edman, A. C., Lexell, J., Sjöström, M. & Squire, J. M. (1988). Structural diversity in muscle fibres of chicken breast. Cell Tissue Res. 251, 281-289.
6. Feinberg, A. P. & Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 13, 6-13.
7. Fürst, D. O., Osborn, M., Nave, R. & Weber, K. (1988). The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten non-repetitive epitopes starting at the Z line extends close to the M line. J. Cell Biol. 106, 1563-1572.
8. Gautel, M., Leonard, K. & Labeit, S. (1993). Phosphorylation of KSP motifs in the C-terminal region of titin in differentiating myoblasts. EMBO J. 12, 3827-3834.
9. Herrmann, B. G. & Frischauf, A. M. (1987). Isolation of genomic DNA. Methods Enzymol. 152, 180-183.
10. Labeit, S. & Kolmerer, B. (1995). Titins, giant proteins in charge of muscle ultrastructure and elasticity. Science, 270, 293-296.
11. Labeit, S., Gautel, M., Lakey A. & Trinick, J. (1992). Towards a molecular understanding of titin. EMBO J. 11, 1711-1716.
12. MacDonald, R. J., Swift, G. H., Przybyla, A. E. & Chirgwin, J. M. (1987). Isolation of RNA using guanidinium salts. Methods Enzymol. 152, 219-226.
13. Maruyama, K. (1994). Connectin, an elastic protein of striated muscle. Biophys. Chem. 50, 73-85.
14. Nave, R., Fürst, D. O. & Weber, K. (1989). Visualization of the polarity of isolated titin molecules: a single globular head on a long thin rod as the M-band anchoring domain? J. Cell Biol. 109, 2177-2187.
15. Pask, H. T., Jones, K. L., Luther, P. K. & Squire, J. M. (1994). M-band structure, M-bridge interactions and contraction speed in vertebrate cardiac muscles. J. Muscle Res. Cell Motil. 15, 633-645.
16. Podlubnaya, Z. A., Shpagina, M. D. & Lednev, V. V. (1989). Manifestation of the stripes of minor proteins location in A bands of rabbit cardiac myofibrils. J. Mol. Biol. 210, 655-658.
17. Saiki, R. K., Scharf, S. J., Faloona, F., Mullis, G. T. & Erlich, H. A. (1985). Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350-1354.
18. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
19. Sjöström, M. & Squire, J. M. (1977). Fine structure of the A band in cryosections. J. Mol. Biol. 109, 49-68.
20. Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.
21. Squire, J. (1981). The Structural Basis of Muscle Contraction, Plenum Press, New York.
22. Suzuki, J., Kimura, S. & Maruyama, K. (1994). Electron microscopic filament lengths of connectin and its fragments. J. Biochem. 116, 406-410.
23. Thornell, L.-E., Carlsson, E., Kugelberg, E. & Grove, B. K. (1987). Myofibrillar M-band structure and composition of physiologically defined rat motor units. Am. J. Physiol. 253, 456-468.
24. Trinick, J. (1994). Titin and nebulin: protein rulers in muscle? Trends Biochem. Sci. 19, 405-409.
25. Vinkemeier, U., Obermann, W., Weber, K. & Fürst, D. O. (1993). The globular head domain of titin extends into the centre of the sarcomeric M band. J. Cell Sci. 106, 319-330.
26. Yanish-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and PUC19 vectors. Gene, 33, 103-119.