Single atomic group in RNA helix needed for positive and negative tRNA synthetase discrimination

Journal of the American Chemical Society

Volumn 118, Issue 10, 1996, Pages 2523-2524

  Liu, Hongjian ^a   Yap, Li Ping ^a,b   Musier Forsyth, Karin ^a  

^a University of Minnesota   (United States)

^b Metabolix Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE;

ARTICLE; ENZYME ACTIVITY; PROTEIN SYNTHESIS; RNA STRUCTURE;

EID: 0029917148     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja954062e     Document Type: Article

References (30)

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13. Pro molecules were prepared by in vitro transcription (Liu. H.; Peterson, R.; Kessler, J.; Musier-Forsyth, K. Nucleic Acids Res. 1995, 23, 165-169). Aminoacylation with alanine was performed using 10-100 nM AlaRS and tRNA concentrations ranging from 0.1-16 μM. AlaRS was purified according to Miller et al. (Miller, W. T.; Hou, Y.-M.; Schimmel, P. Biochemistry 1991, 30, 2635-2641). Kinetic parameters were estimated from Lineweaver-Burk plots and are averages of two determinations.
14. Pro molecules were prepared by in vitro transcription (Liu. H.; Peterson, R.; Kessler, J.; Musier-Forsyth, K. Nucleic Acids Res. 1995, 23, 165-169). Aminoacylation with alanine was performed using 10-100 nM AlaRS and tRNA concentrations ranging from 0.1-16 μM. AlaRS was purified according to Miller et al. (Miller, W. T.; Hou, Y.-M.; Schimmel, P. Biochemistry 1991, 30, 2635-2641). Kinetic parameters were estimated from Lineweaver-Burk plots and are averages of two determinations.
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