X-ray crystal structure of the two site-specific mutants Ile7Ser and Phe110Ser of azurin from Pseudomonas aeruginosa

Journal of Molecular Biology

Volumn 255, Issue 3, 1996, Pages 362-366

  Hammann, Christian ^a,c   Messerschmidt, Albrecht ^a   Huber, Robert ^a   Nar, Herbert ^a   Gilardi, Gianfranco ^b   Canters, Gerard W ^b  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b LEIDEN UNIVERSITY   (Netherlands)

^c INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

Author keywords

Azurin; Copper; Electron transfer; Fluorescence; Protein structure

Indexed keywords

AZURIN; COPPER; ISOLEUCINE; MUTANT PROTEIN; PHENYLALANINE; SERINE; TRYPTOPHAN;

ARTICLE; DIELECTRIC CONSTANT; ELECTRON TRANSPORT; FLUORESCENCE SPECTROSCOPY; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; X RAY CRYSTALLOGRAPHY;

EID: 0029916957     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0029     Document Type: Editorial

References (20)

1. Brünger, A. T. (1990). X-Plor Manual. Yale University, New Haven, CT.
2. (1994). The CCP4 suite: programs for protein crystallography. Acta. Crystallog. Sect. D, 50, 760-763.
3. Farver, O. & Pecht, I. (1989). Long-range intramolecular electron transfer in azurins. Proc. Natl. Acad. Sci. USA. 86, 6968-6972.
4. Farver, O. & Pecht, I. (1992). Long range intramolecular electron transfer in azurins. J. Am. Chem. Soc. 114, 5764-5767.
5. Farver, O., Skov, L. K., van de Kamp, M., Canters, G. W. & Pecht, I. (1992). The effect of driving force on intramolecular electron transfer in proteins. Eur. J. Biochem 210, 399-403.
6. Gilardi, G., Mei, G., Rosato, N., Canters, G. W. & Finazzi-Agró, A. (1994). Unique environment of Trp48 in Pseudomonas aeruginosa azurin as probed by site-directed mutagenesis and dynamic fluorescence spectroscopy. Biochemistry, 33, 1425-1432.
7. Huber, R. & Kopfmann, G. (1969). Experimental absorption corrections: results. Acta Crystallog. sect. A, 25, 143-152.
8. Jones, T. A. (1978). A graphics model building and refinement system for macromolecules. J. Appl. Crystallog. 15, 24-31.
9. Kim, S. T., Heelis, P. F., Okamura, T., Hirata, Y., Mataga, N. & Sancar, A. (1991). Energy transfer and electron transfer in photolyase. Biochemistry, 30, 11262-11270.
10. Leslie, A. G. W. (1990). In Crystallographic Computing (Moras, D., Podjarny A. D. & Thierry, J. C., eds), vol. 5, pp. 50-61, Oxford University Press, Oxford.
11. Luzzati, P. V. (1952). Traitement statistique des erreur dans la determination des structures cristallines. Acta Crystallog. 5, 802-810.
12. Marcus, R. A. & Sutin, N. (1985). Electron transfer in chemistry and biology. Biochim. Biophys. Acta, 811, 268-322.
13. Messerschmidt, A. & Pflugrath, J. W. (1987). Crystal orientation and X-ray pattern prediction routines for area-detector diffractometer systems in macromol-ecular crystallography. J. Appl. Crystallog. 23, 306-315.
14. Messerschmidt, A., Schneider, M. & Huber, R. (1990). ABSCOR: A scaling and absorption correction program for the FAST area detector. J. Appl. Crystallog. 20, 306-315.
15. Nar, H., Messerschmidt, A., Huber, R., van de Kamp, M. & Canters, G. W. (1991). Crystal structure analysis of oxidised Pseudomonas aeruginosa azurin at pH 5.5 and pH 9.0. J. Mol. Biol. 221, 765-772.
16. Navaza, J. (1994). AMoRe: an automated package for molecular replacement. Acta Crystallog. sect. A, A50, 157-163.
17. Parr, S. R., Barber, D., Greenwood, C., Philips, B. W. & Melling, J. (1976). A purification procedure for the soluble cytochrome oxidase and some other respiratory proteins from Pseudomonas aeruginosa. Biochem. J. 157, 423-430.
18. Petrich, J. W., Longworth, J. W. & Fleming, G. R. (1987). Internal motion and electron transfer in proteins: A picosecond fluorescence study of three homologous azurins. Biochemistry, 26, 2711-2722.
19. Steigemann, W. (1974). Die Entwicklung und Anwendung von Rechenverfahren und Rechenprogrammen zur Struktur analyse von Proteinen am Beispiel des Trypsin-Trypsin Inhibitor Komplex es, des freien Inhibitors und der L-Asparaginase. PhD thesis, Technische Universität München.
20. Sweeney J. A., Harmon, P. A., Asher, S. A., Hutnik, C. M. & Szabo, A. G. (1991). UV resonance raman examination of the azurin tryptophan environment and energy relaxation pathways. J. Am. Chem. Soc. 113, 7531-7537.