Flow cytometric ratio analysis of the Hoechst 33342 emission spectrum: Multiparametric characterization of apoptotic lymphocytes

Journal of Immunological Methods

Volumn 189, Issue 2, 1996, Pages 157-171

  Chiu, Laura ^a   Cherwinski, Holly ^a   Ransom, John ^a   Dunne, John F ^a,b  

^a ROCHE BIOSCIENCE   (United States)

^b NONE   (United States)

Author keywords

Activation; Apoptosis; Chromatin condensation; Flow cytometry; Lymphocyte regulation; Programmed cell death

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; DEXAMETHASONE; DNA FRAGMENT; HOE 33342; INTERLEUKIN 2 RECEPTOR; IONOMYCIN; STAUROSPORINE;

ADULT; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CYCLE; CHROMATIN CONDENSATION; DNA DEGRADATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; NONHUMAN; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; THYMOCYTE;

EID: 0029916935     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00214-6     Document Type: Article

References (34)

1. Akbar, A.N., Salmon, M., Savill, J. and Janossy, G. (1993) A possible role for bcl-2 in regulating T-cell memory - a 'balancing act' between cell death and survival. Immunol. Today 14, 526.
2. Akbar, A.N., Borthwick, N., Salmon, M., Gombert, W., Bofill, M., Shamsadeen, N., Pilling, D., Pett, S., Grundy, J.E. and Janossy, G. (1993) The significance of low bcl-2 expression by CD45RO T cells in formanl individuals and patients with acute viral infections. The role of apoptosis in T cell memory. J. Exp. Med. 178, 427.
3. Andjelic, S., Jain, J. and Nikolic-Zugic J. (1993) Immature thymocytes become sensitive to calcium-mediated apoptosis with the onset of CD8, CD4, and the T cell receptor expression: A role for bcl-2? J. Exp. Med. 178, 1745.
4. Belloc, F., Dumain, P., Boisseau, M.R., Jalloustre, C., Reiffers, J., Bernard, P. and Lacombe, F. (1994) A flow cytometric method using hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells. Cytometry 17, 59.
5. Bertrand, R., Solary, E., O'Connor, P., Kohn, K.W. and Pommier, Y. (1994) Induction of a common pathway of apoptosis by staurosporine. Exp. Cell Res. 211, 314.
6. Cohen, J.J. (1993). Apoptosis. Immunol. Today 14, 126.
7. Cohen, J.J. and Duke, R.C. (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. J. Immunol. 132, 38.
8. Cohen, G.M., Sun, X.-M., Snowden, R.T., Ormerod, M.G. and Dinsdale, D. (1993) Identification of a transitional preapoptotic population of thymocytes. J. Immunol. 151, 566.
9. Darzynkiewicz, Z., Bruno, S., DelBino, G., Gurizyca, W., Hotz, M.A., Lassota P. and Traganos, F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795.
10. Darzynkiewicz, Z., Li, X. and Gong, J. (1994) Assays of cell viability. Discrimination of cells dying by apoptosis. In: Z. Darzynkiewicz, H.A. Crissman and J.R. Robinson (Eds.), Methods in Cell Biology: Flow Cytometry, 2nd edn. Academic Press, New York.
11. Del Buono, B.J., Williamson, P.L. and Schlegel, R.A. (1986) Alterations in plasma membrane organization during lymphocyte differentiation. J. Cell. Physiol. 126, 379.
12. Dive, C., Gregory, C.D., Phipps, D.J., Evans, D.L., Miller, A.E. and Wyllie, A.H. (1992) Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim. Biophys. Acta 1133, 275.
13. Ellwart, J.W. and Dormer P. (1990) Vitality measurement using spectrum shift in Hoechst 33342 stained cells. Cytometry 11, 239.
14. Elstein, K.H. and Zucker, R.M. (1994) Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations. J. Immunol. Methods 154, 99.
15. Fadok, V.A., Savill, J.S., Haslett, C., Bratton, D.L., Doherty, D.E., Campbell, P.A. and Hanson, P.M. (1992) Different populations of macrophages use either the vitronectin receptor or the the phosphatidyl serine receptor to recognize and remove apoptotic cells. J. Immunol. 149, 4029.
16. Fadok, V.A., Voelker, D.R., Campbell, P.A., Cohen, J.J., Bratton, D.L. and Hanson, P.M. (1992) Exposure of phsphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol. 148, 2207.
17. Gorczyca, W., Gong, J. and Darzynkiewicz, Z. (1993) Detection of DNA strand breaks in individual apoptic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53, 1945.
18. Hardin, J.A., Sherr, D.H., DeMaria, M. and Lopez, P.A. (1992) A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing DNA fragmentation. J. Immunol. Methods 154, 99.
19. Koopman, G., Reutelingsperger, C.P.M., Kuijten, G.A.M., Keehnen, R.M.J., Pals, S.T. and van Oers, M.H.J. (1995) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415.
20. Latt, S.A. and Stellen, G. (1976) Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24, 24.
21. Lyons, A.B., Samuel, K., Sanderson, A. and Maddy, A.H. (1992) Simultaneous analysis of imunophenotype and apoptosis of murine thymocytes by single laser flow cytometry. Cytometry 13, 809.
22. McEvoy, L., Schegel, R.A., Williamson, P. and Del Buono, B.J. (1988) Merocyanine 540 as a flow cytometric probe of lipid organization in leukocytes. J. Leuk. Biol. 44, 337.
23. Mower, D.A., Peckham, D.W., Illera, V.A., Fishbaugh, J.K., Stunz, L.L. and Ashman, R.F. (1994) Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis. J. Immunol. 152, 4832.
24. Nunez, G., Merino, R., Grillot, D. and Gonzalez-Garcia, M. (1994) Bcl-2 and Bcl-x: Regulatory switches for lymphoid death and survival. Immunol. Today 15, 582.
25. Ormerod, M.G., Sun, X.-M., Snowden, R.T., Davies, R., Fearnhead, H. and Cohen, G.M. (1993) Increased membrane permeability of apoptotic thymocytes: a flow cytometric study. Cytometry 14, 595.
26. Schmid, I., Uittenbogaart, C.H., Keld, B. and Giorgi, J.V. (1994) A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170, 145.
27. Schmid, I., Uittenbogaart, C.H. and Giorgi, J.V. (1994) Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15, 12.
28. Stilwell, W., Wassall, S.R., Dumaual, A.C., Ehringer, W.D., Browning, C.W. and Jenski, L.J. (1993) Use of merocyanine (MC540) in quantifying lipid domains and packing in phospholipid vessicles and tumor cells. Biochim. Biophys. Acta 1146, 136.
29. Stokke, T. and Steen, H.B. (1985) Multiple binding modes for Hoechst 33258 to DNA. J. Histochem. Cytochem. 33, 333.
30. + thymocytes by flow cytometry. J. Immunol. Methods 137, 79.
31. Walker, P.R., Kwast-Welfield, J., Gourdeau, H., Leblanc, J., Neugebauer, W. and Sikorska, M. (1993) Relationship between apoptosis and the cell cycle in lymphocytes: Roles of protein kinase C, tyrosine phosphorylation and AP1. Exp. Cell Res. 207, 142.
32. Watson, J.V, Nakeff, A., Chambers, S.H. and Smith, P. (1985) Flow cytometric fluorescence emission spectrum analysis of Hoechst-33342-stained DNA in chicken thymocytes. Cytometry 6, 310.
33. Williamson, P., Mattocks, K. and Schlegel, R.A. (1983) Merocyanine 540, a fluorescent probe sensitive to lipid packing. Biochem. Biophys. Acta 732, 387.
34. Wyllie, A.H. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284, 555.