Substitution of mucAB or rumAB for umuDC alters the relative frequencies of the two classes of mutations induced by a site-specific T-T cyclobutane dimer and the efficiency of translesion DNA synthesis

Journal of Bacteriology

Volumn 178, Issue 9, 1996, Pages 2559-2563

  Szekeres Jr , Edward S ^a   Woodgate, Roger ^b   Lawrence, Christopher W ^a,c  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOBUTANE; DIMER; DNA DIRECTED DNA POLYMERASE ALPHA; MUTAGENIC AGENT;

ARTICLE; BACTERIAL GENETICS; DNA DAMAGE; DNA SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET RADIATION;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA REPAIR; DNA REPLICATION; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OPERON; POINT MUTATION; PYRIMIDINE DIMERS; ULTRAVIOLET RAYS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029916565     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.9.2559-2563.1996     Document Type: Article

References (41)

1. Bailone, A., S. Sommer, J. Knezevic, M. Dutreix, and R. Devoret. 1991. A RecA protein mutant deficient in its interaction with the UmuDC complex. Biochimie 73:479-484.
2. Banerjee, S. K., A. Borden, R. B. Christensen, J. E. LeClerc, and C. W. Lawrence. 1990. SOS-dependent replication past a single trans-syn T-T cyclobutane dimer gives a different mutation spectrum and increased error rate compared with replication past this lesion in uninduced cells. J Bacteriol 172:2105-2112.
3. Banerjee, S. K., R. B. Christensen, C. W. Lawrence, and J. E. LeClerc. 1988. Frequency and spectrum of mutations produced by a single cis-syn thymine-thymine cyclobutane dimer in a single-stranded vector. Proc. Natl. Acad. Sci. USA 85:8141-8145.
4. Blanco, M., G. Herrera, and V. Aleixandre. 1986 Different efficiency of UmuDC and MucAB proteins in UV light induced mutagenesis in Escherichia coli. Mol. Gen. Genet. 205:234-239
5. Blanco, M., G. Herrera, P. Collado, J. E. Rebollo, and L. M. Botella. 1982. Influence of RecA protein on induced mutagenesis Biochimie 64:633-636
6. Bridges, B. A., R. P. Mottershead, and S. G. Sedgwick. 1976. Mutagenic repair in Escherichia coli III. Requirement for a function of DNA polymerase III in ultraviolet light mutagenesis. Mol. Gen. Genet. 144:53-58.
7. Bridges, B. A., and R. Woodgate. 1984. Mutagenic repair in Escherichia coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error m UV-mutagenesis. Mol. Gen. Genet. 196:364-366.
8. Bridges, B. A., and R. Woodgate. 1985. The two-step model of bacterial UV mutagenesis. Mutat. Res. 150:133-139.
9. Bridges, B. A., and R. Woodgate. 1985. Mutagenic repair in Escherichia coli: products of the recA gene and of the umuD and umuC genes act at different steps in UV-induced mutagenesis Proc. Natl. Acad. Sci. USA 82:4193-4197.
10. Burckhardt, S., R. Woodgate, R. Scheuermann, and H. Echols. 1988. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA. Proc. Natl Acad. Sci. USA 85:1811-1815.
11. Churchward, G., D. Belin, and Y. Nagamine. 1984. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31:165-171.
12. Doyle, N., and P. Strike. 1995. The spectra of base substitutions induced by the impCAB, mucAB and umuDC error-prone repair operons differ following exposure to methyl methane sulfonate. Mol. Gen. Genet. 247:735-741.
13. Ennis, D. G., A. S. Levine, W. H. Koch, and R. Woodgate. 1995. Analysis of recA mutants with altered SOS functions. Mutat. Res. 336:39-48.
14. Foster, P. L., and A. D. Sullivan. 1988. Interactions between epsilon, the proofreading subunit of DNA polymerase III, and proteins in the SOS response of Escherichia coli. Mol. Gen. Genet. 214:467-473.
15. Fowler, R. G., L. McGinty, and K. E. Mortelmans. 1981. Mutational specificity of ultraviolet light in Escherichia coli with and without the plasmid pKM101. Genetics 99:25-40.
16. Frank, E. G., J. Hauser, A. S. Levine, and R. Woodgate. 1993. Targeting of the UmuD, UmuD′ and MucA′ mutagenesis proteins to DNA by RecA protein. Proc. Natl. Acad Sci. USA 90:8169-8173.
17. Friedberg, E. C., G. C. Walker, and W. Siede. 1995. DNA repair and mutagenesis. American Society for Microbiology, Washington, D C.
18. Gibbs, P., M. Horsfall, A. Borden, B. J. Kilbey, and C. W. Lawrence. 1993. Understanding spectra of UV-induced mutations: studies with individual photoproducts, p. 357-362. In A. Shima, M. Ichahashi, Y. Fujiwara, and H. Takebe (ed), Frontiers of photobtology. Excerpta Medica, Amsterdam.
19. Hagensee, M. E., T. Timme, S. K. Bryan, and R. E. Moses. 1987. DNA polymerase III of Escherichia coli is required for UV and ethyl methanesulfonate mutagenesis. Proc. Natl. Acad Sci. USA 84:4195-4199.
20. Hauser, J., A. S. Levine, D. G. Ennis, K. M. Chumakov, and R. Woodgate. 1992. The enhanced mutagenic potential of the MucAB proteins correlates with the highly efficient processing of the MucA protein. J Bacteriol. 174: 6844-6851.
21. Ho, C., O. I. Kulaeva, A. S. Levine, and R. Woodgate. 1993. A rapid method for cloning mutagenic DNA repair genes: isolation of umu-complementing genes from multidrug resistance plasmids R391, R446b, and R471a. J. Bacteriol. 173:5411-5419.
22. Horsfall, M. J., and C. W. Lawrence. 1994. Accuracy of replication past the T-C (6-4) adduct. J. Mol. Biol. 235:465-471.
23. Kulaeva, O. I., J. C. Wootton, A. S. Levine, and R. Woodgate. 1995. Characterization of the umu-complementing operon from R391. J. Bacteriol. 177:2737-2743
24. Lawrence, C. W., S. K. Banerjee, A. Borden, and J. E. LeClerc. 1990. T-T cyclobutane dimers are misinstructive, rather than noninstructive, mutagenic lesions. Mol. Gen. Genet 222:166-168.
25. Lawrence, C. W., A. Borden, S. K. Banerjee, and J. E. LeClerc. 1990. Mutation frequency and spectrum resulting from a single abasic site in a single stranded vector. Nucleic Acids Res. 18:2153-2157.
26. Lawrence, C. W., A. Borden, and R. Woodgate. Analysis of the mutagenic potential of the UmuDC. MucAB and RumAB proteins using a site specific abasic lesion. Mol. Gen. Genet., in press.
27. Leclerc, J. E., A. Borden, and C. W. Lawrence. 1991. The thymine-thymine pyrimidine-pyrimidone (6-4) ultraviolet light photoproduct is highly mutagenic and specifically induces 3′ thymine-to-cytosine transitions in Escherichia coli. Proc. Natl. Acad. Sci. USA 88:9685-9689.
28. Murli, S., and G. C. Walker. 1993. SOS mutagenesis. Curr. Opin. Genet. Dev. 3:719-725
29. Nohmi, T., J. R. Battista, L. A. Dodson, and G. C. Walker. 1988. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc. Natl. Acad Sci USA 85:1816-1820.
30. Palejwala, V. A., R. W. Rzepka, and M. Z. Humayuu. 1993. UV irradiation of Escherichia coli modulates mutagenesis at a site-specific ethenocytosine residue on M13 DNA. Biochemistry 32:4112-4120.
31. Perry, K., S. Elledge, B. Mitchell, L. Marsh, and G. Walker. 1985. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. Proc. Natl. Acad. Sci. USA 82:4331-4335.
32. Rajagopalan, M., C. Lu, R. Woodgate, M. O'Donnell, M. F. Goodman, and H. Echols. 1992. Activity of the purified mutagenesis proteins UmuC, UmuD′, and RecA in replicative bypass of an abasic DNA lesion by DNA polymerase III Proc. Natl. Acad. Sci. USA 89:10777-10781.
33. Shinagawa, H., H. Iwasaki, T. Kato, and A. Nakata. 1988. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc. Natl. Acad. Sci. USA 85:1806-1810.
34. Slater, S. C., and R. Maurer. 1991. Requirements for bypass of UV-induced lesions in single-stranded DNA of bacteriophage phi X174 in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 88:1251-1255.
35. Sweasy, J. B., E. M. Witkin, N. Sinha, and V. Roegner-Maniscalco. 1990. RecA protein of Escherichia coli has a third essential role in SOS mutator activity. J. Bacteriol. 172:3030-3036.
36. Tadmor, Y., R. Ascarelli-Goeli, R. Skaliter, and Z. Livneh. 1992 Overproduction of the p subunit of DNA polymerase III holoenzyme reduces UV mutagenesis in Escherichia coli. J. Bacteriol. 174:2517-2524.
37. Watanabe, M., T. Nohmi, and T. Ohta. 1994. Effects of the umuDC, mucAB, and samAB operons on the mutational specificity of chemical mutagenesis in Escherichia coli. I. Frameshift mutagenesis. Mutat. Res 314:27-37.
38. Woodgate, R., B. A. Bridges, G. Herrera, and M. Blanco. 1987. Mutagenic DNA repair in Escherichia coli XIII Proofreading exonuclease of DNA polymerase III holoenzyme is not operational during UV mutagenesis. Mutat Res. 183:31-37
39. Woodgate, R., and D. G. Ennis. 1991. Levels of chromosomally encoded Umu proteins and requirements for in vivo UmuD cleavage Mol. Gen. Genet. 229:10-16.
40. Woodgate, R., M. Rajagopalan, C. Lu, and H. Echols. 1989. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD′. Proc. Natl Acad. Sci. USA 86:7301-7305.
41. Woodgate, R., and S. G. Sedgwick. 1992. Mutagenesis induced by bacterial UmuDC proteins and their plasmid homologues. Mol. Microbiol. 6:2213-2218