High level secretion of wild-type and mutant forms of human proapoA-I using baculovirus-mediated Sf-9 cell expression

Journal of Lipid Research

Volumn 37, Issue 3, 1996, Pages 673-683

  Sorci Thomas, Mary G ^a   Parks, John S ^a   Kearns, Mary W ^a   Pate, Gregory N ^a   Zhang, Chi ^a   Thomas, Michael J ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

apolipoprotein A I; baculovirus expression; cholesterol; cholesteryl ester; discoidal complexes; high density lipoproteins; lecithin:cholesterol acyltransferase; Sf 9 cells

Indexed keywords

APOLIPOPROTEIN; CHOLESTEROL; CHOLESTEROL ESTER; HIGH DENSITY LIPOPROTEIN CHOLESTEROL; LIPID BINDING PROTEIN; PHOSPHATIDYLCHOLINE STEROL ACYLTRANSFERASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE MUTATION; LIPOPROTEIN BLOOD LEVEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

ANIMALS; APOLIPOPROTEIN A-I; APOLIPOPROTEINS A; BACULOVIRIDAE; CELL LINE; CIRCULAR DICHROISM; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; HUMANS; LIPOPROTEINS, HDL; MASS SPECTROMETRY; MOLECULAR WEIGHT; MUTATION; PHOSPHATIDYLCHOLINE-STEROL O-ACYLTRANSFERASE; PROTEIN PRECURSORS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0029916540     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. Fielding, C. J., and P. E. Fielding. 1995. Molecular physiology of reverse cholesterol transport. J. Lipid Res. 36: 211-228.
2. Jonas, A. 1991. Lecithin:cholesterol acyltransferase in the metabolism of high density lipoproteins. Biochim. Biophys. Acta. 1084: 205-220.
3. Segrest, J. P., M. K. Jones, H. De Loof, C. G. Brouillette, Y. V. Venkatachalapathi, and G. M. Anantharamaiah. 1992. The amphipathic helix in the exchangeable apolipoproteins: a review of secondary structure and function. J. Lipid Res. 33: 141-166.
4. Rall, S. C., Jr., K. H. Weisgraber, R. W. Mahley, Y. Ogawa, C. J. Fielding, G. Utermann, J. Haas, A. Steinmetz, H-J. Menzel, and G. Assmann. 1984. Abnormal lecithin:cholesterol acyltransferase activation by a human apolipoprotein A-I variant in which a single lysine residue is deleted. J. Biol. Chem. 259: 10063-10070.
5. Jonas, A., A. von Eckardstein, K. E. Kezdy, A. Steinmetz, and G. Assmann. 1991. Structural and functional properties of reconstituted high density lipoprotein discs prepared with six apolipoprotein A-I variants. J. Lipid Res. 32: 97-106.
6. Vanloo, B., J. Taveirne, J. Baert, G. Lorent, L. Lins, J-M. Ruyschaert, and M. Rosseneu. 1992. LCAT activation properties of apoA-I CNBr fragments and conversion of discoidal complexes into spherical particles. Biochim Biophys. Acta. 1128: 258-266.
7. Ji, Y., and A. Jonas. 1995. Properties of an N-terminal proteolytic fragment of apolipoprotein AI in solution and in reconstituted high density lipoproteins. J. Biol. Chem. 270: 11290-11297.
8. Anantharamaiah, G. M., Y. V. Venkatachalapathi, C. G. Brouillette, and J. P. Segrest. 1990. Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-I. Arteriosclerosis. 10: 95-105.
9. Segrest, J. P., D. W. Garber, C. G. Brouillette, S. C. Harvey, and G. M. Anantharamaiah. 1994. The amphipathic α-helix: a multifunctional structural motif in plasma apolipoproteins. Adv. Protein Chem. 45: 303-369.
10. Banka, C. L., D. J. Bonnet, A. S. Black, R. S. Smith, and L. K. Curtiss. 1991. Localization of an apolipoprotein A-I epitope critical for activation of lecithin:cholesterol acyltransferase. J. Biol. Chem. 35: 23886-23892.
11. Meng, Q-H., L. Calabresi, J-C. Fruchart, and M. L. Marcel. 1993. Apolipoprotein A-I domains involved in the activation of lecithin:cholesterol acyltransferase: importance of the central domain. J. Biol. Chem. 268: 16966-16973.
12. 13C NMR studies of lysine ionization behavior. J. Biol. Chem. 267: 25830-25838.
13. 13C NMR studies using selectively labeled peptides. Biochemistry. 34: 9219-9226.
14. Wald, J. H., E. S. Krul, and A. Jonas. 1990. Structure of apolipoprotein A-I in three homogeneous, reconstituted high density lipoprotein particles. J. Biol. Chem. 265: 20037-20043.
15. Jonas, A., K. E. Kezdy, and J. H. Wald. 1989. Defined apolipoprotein A-I conformations in reconstituted high density lipoprotein discs. J. Biol. Chem. 264: 4818-4824.
16. Minnich, A., X. Collet, A. Roghani, C. Cladaras, R. L. Hamilton, C. J. Fielding, and V. I. Zannis. 1992. Site-directed mutagenesis and structure-function analysis of the human apolipoprotein AI: relation between lecithin:cholesterol acyltransferase activation and lipid binding. J. Biol. Chem. 267: 16553-16560.
17. Sorci-Thomas, M., M. W. Kearns, and J. P. Lee. 1993. Apolipoprotein A-I domains involved in lecithin:cholesterol acyltransferase activation: structure-function relationships. J. Biol. Chem. 268: 21403-21409.
18. Schmidt, H. H-J., A. T. Remaley, J. A. Stonik, R. Ronan, A. Wellmann, F. Thomas, L. A. Zech, H. B. Brewer, and J. M. Hoeg. 1995. Carboxyl-terminal domain truncation alters apolipoprotein A-I in vivo catabolism. J. Biol. Chem. 270: 5469-5475.
19. Brouillette, C. G., and G. M. Anantharamaiah. 1995. Structural models of human apolipoprotein A-I. Biochim. Biophys. Acta. 1256: 103-129.
20. Nolte, R. T., and D. Atkinson. 1992. Conformational analysis of apolipoprotein A-I and E-3 based on primary sequence and circular dichroism. Biophys. J. 63: 1221-1239.
21. Marcel, Y. L., P. R. Provost, H. Koa, E. Raffai, N. Vu Dac, J-C. Fruchart, and E. Rassart. 1991. The epitopes of apolipoprotein A-I define distinct structural domains including a mobile middle region. J. Biol. Chem. 266: 3644-3653.
22. Breiter, D. R., M. R. Kanost, M. M. Benning, G. Wesenberg, J. H. Law, M. A. Wells, I. Rayment, and H. M. Holden. 1991. Molecular structure of an apolipoprotein determined at 2.5-Å resolution. Biochemistry. 30: 603-608.
23. Weisgraber, K. H. 1994. Apolipoprotein E: structure-function relationships. Adv. Protein Chem. 45: 249-302.
24. Cheung, P., and L. Chan. 1983. Nucleotide sequence of cloned cDNA of human apolipoprotein A-I. Nucleic Acids Res. 11: 3703-3715.
25. Sarkar, G., and S. S. Sommer. 1990. The "Megaprimer" method of site-directed mutagenesis. BioTechniques. 8: 404-407.
26. Zhong, D., and S. P. Bajaj. 1993. A PCR-based method for site-specific domain replacement that does not require restriction recognition sequences. BioTechniques. 15: 874-878.
27. Koritnik, D. L., and L. L. Rudel. 1983. Measurement of apolipoprotein A-I concentration in nonhuman primate serum by enzyme-linked immunosorbent assay. J. Lipid Res. 24: 1639-1645.
28. Hughes, T. A., M. A. Moore, P. Neame, M. F. Medley, and B. H. Chung. 1988. Rapid quantitative apolipoprotein analysis by gradient ultracentrifugation and reversed-phase high performance liquid chromatography. J. Lipid Res. 29: 363-376.
29. Weinberg, R., C. Patton, and B. DaGue. 1988. Analytic and preparative separation of apolipoproteins A-I, A-II, and A-IV by reverse phase high pressure liquid chromatography. J. Lipid Res. 29: 819-824.
30. Edelstein, C., C. T. Lam, and A. M. Scanu. 1972. On the subunit structure of the protein of human serum high density lipoprotein. J. Biol. Chem. 247: 5842-5849.
31. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
32. Chen, Y-H., J. T. Yang, and H. M. Martinez. 1972. Determination of the secondary structure of proteins by circular dichroism and optical rotatory dispersion. Biochemistry. 11: 4120-4131.
33. Parks, J. S., T. Y. Thuren, and J. D. Schmitt. 1992. Inhibition of lecithin:cholesterol acyltransferase activity by synthetic phosphatidylcholine species containing eicosapentaenoic acid or docosahexaenoic acid in the sn-2 position. J. Lipid Res. 33: 879-887.
34. Fiske, C H., and Y. SubbaRow. 1925. Colorimetric determination of phosphorus. J. Biol. Chem. 66: 357-400.
35. Auerbach, B. J., J. S. Parks, and D. Applebaum-Bowden. 1990. A rapid and sensitive micro-assay for the enzymatic determination of plasma and lipoprotein cholesterol. J. Lipid Res. 31: 738-742.
36. Miller, K.R., J. Wang, M. Sorci-Thomas, R.A. Anderson, and J.S. Parks. 1996. Glycosylation structure and enzyme activity of lecithin:cholesterol acyltransferase from human plasma, HepG2 cells, and baculoviral and Chinese hamster ovary cell expression systems. J. Lipid Res. 37: 551-561.
37. Rainwater, D. L., D. W. Andres, A. L. Ford, W. F. Lowe, P. J. Blanche, and R. M. Krauss. 1992. Production of polyacrylamide gradient gels for the electrophoretic resolution of lipoproteins. J. Lipid Res. 33: 1876-1881.
38. Lux, S. E., R. Hirz, R. I. Shrager, and A. M. Gotto. 1972. The influence of lipid on the conformation of human plasma high density apolipoproteins. J. Biol. Chem. 247: 2598-2606.
39. Milano dimer: the introduction of an interchain disulfide bridge remarkably alters the physiochemical properties of apolipoprotein A-I. J. Biol. Chem. 269: 32168-32174.
40. Pyle, L. E., P. Barton, Y. Fujiwara, A. Mitchell, and N. Fidge. 1995. Secretion of biologically active human proapolipoprotein A-I in a baculovirus-insect cell system: protection from degradation by protease inhibitors. J. Lipid Res. 36: 2355-2361.
41. Monaco, L., H. M. Bond, K. E. Howell, and R. Cortese. 1987. A recombinant apoA-I protein A hybrid reproduces the binding parameters of HDL to its receptor. EMBO J. 6: 3253-3260.
42. Moguilevsky, N., C. Roobol, R. Loriau, J-P. Guillaume, P. Jacobs, A. Cravador, A. Herzog, L. Brouwers, A. Scarso, P. Gilles, L. Holmquist, L. A. Carlson, and A. Bollen. 1987. Production of human recombinant proapolipoprotein A-I in Escherichia coli. DNA. 8: 429-436.
43. Isacchi, A., P. Sarmientos, R. Lorenzetti, and M. Soria. 1989. Mature apolipoprotein AI and its precursor proApoAI: influence of the sequence at the 5′ end of the gene on the efficiency of expression in Escherichia coli. Gene. 81: 129-137.
44. Bruhn, H., and W. Stoffel. 1991. An approach to the functional analysis of lecithin:cholesterol acyltransferase: activation by recombinant normal and mutagenized apolipoprotein A-I. Biol. Chem. Hoppe Seyler. 372: 225-234.
45. Dixon, J. L., R. Battini, S. Ferrari, C. M. Redman, and D. Banerjee. 1989. Expression and secretion of chicken apolipoprotein AI in transfected COS cells. Biochim. Biophys. Acta. 1009: 47-53.
46. Mallory, J. B., P. J. Kushner, A. A. Protter, C. L. Cofer, D. L. Appleby, K. Lau, J. W. Shilling, and J-L. Vigne. 1987. Expression and characterization of human apolipoprotein A-I in Chinese hamster ovary cells. J. Biol. Chem. 262: 4241-4247.
47. Moguilevsky, N., J-P. Guillaume, F. Varsalona, M. Bulinckx, and A. Bollen. 1994. Correct in vivo processing of a chimeric ubiquitin-proapolipoprotein A-I fusion protein in baculovirus-infected insect cells. J. Biotech. 32: 39-43.
48. Milano reduces intimal thickening after balloon injury in hypercholesterolemia rabbits. Circulation. 90: 1935-1941.