Rapid protocol for template preparation of large numbers of clones

BioTechniques

Volumn 20, Issue 4, 1996, Pages 554-558

  Bartoli, F ^a   Nuzzo, M ^a   Pezzanera, M ^a   Nicosia, A ^a   Monaci, P ^a  

^a IRBM   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; DNA DETERMINATION; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

BACTERIOPHAGES; BASE SEQUENCE; CLONING, MOLECULAR; DNA, VIRAL; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TIME FACTORS;

EID: 0029916198     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

1. Bachmann, B., W. Luke and G. Humnsmann. 1990. Improvement of PCR amplified DNA sequencing with the aid of detergents. Nucleic Acids Res. 18:1309.
2. Felici, F., L. Castagnoli, A. Musacchio, R. Jappelli and G. Cesareni. 1991. Selection of antibody ligands from a large library of oligopeptides expressed on a multivalent exposition vector. J. Mol. Biol 222:301-310.
3. Kusukawa, N., T. Uemori, K. Asada and I. Kato. 1990 Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. BioTechniques 9:66-72.
4. Lee, A.B. and T.A. Cooper. 1995. Improved direct PCR screen for bacterial colonies: wooden toothpicks inhibit PCR amplification. BioTechniques 18:225-226.
5. Sambrook, J., Fritsch, E.F. and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Smith, G.P. 1991. Surface presentation of protein epitopes using bacteriophage expression systems. Curr. Opin. Biotechnol. 2:668-673.