Expression, lipoylation and structure determination of recombinant pea H-protein in Escherichia coli

European Journal of Biochemistry

Volumn 236, Issue 1, 1996, Pages 27-33

  Macherel, D ^a   Bourguinon, J ^a   Forest, E ^a   Faure, M ^a   Cohen Addad, C ^a   Douce, R ^a  

^a DIF   (France)

Author keywords

Glycine decarboxylase; Lipoylation; Overexpression; X ray structure

Indexed keywords

CARBOXYLYASE; CHLORAMPHENICOL; GLYCINE DEHYDROGENASE (DECARBOXYLATING); H PROTEIN; LIGASE; MULTIENZYME COMPLEX; RECOMBINANT PROTEIN; SYNTHETIC DNA; THIOCTIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; MASS SPECTROMETRY; NONHUMAN; PEA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; X RAY ANALYSIS;

PISUM SATIVUM;

EID: 0029915259     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00027.x     Document Type: Article

References (33)

1. Ali, S. T., Moir, A. J. G., Ashton, P. R., Engel, P. C. & Guest, J. R. (1990) Octanoylation of the lipoyl domains of the pyruvate dehydrogenase complex in a lipoyl‐deficient strain of Escherichia coli, Mol. Microbiol. 4, 943–950.
2. Berg, A., De Kok, A. & Vervoort, J. (1994) Sequential 1 H and 15 N nuclear magnetic resonance assignments and secondary structure of the N‐terminal lipoyl domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii, Eur. J. Biochem. 221, 87–100.
3. Bourguignon, J. B., Neuburger, M. & Douce, R. (1988) Resolution and characterization of the glycine‐cleavage reaction in pea leaf mitochondria, Biochem. J. 255, 169–178.
4. Brockelhurst, S. & Perham, R. N. (1993) Prediction of the three‐dimensional structures of the biotinylated domain from the pyruvate carboxylase of yeast and of the lipoylated H protein from the glycine cleavage system of pea leaf: a new, automated method for the prediction of protein tertiary structures, Protein Sci. 2, 626–639.
5. Brookfield, D. E., Green, J., Ali, S. T., Machado, R. S. & Guest, J. R. (1991) Evidence for two protein‐lipoylation activities in Escherichia coli, FEBS Lett. 295, 13–16.
6. Brünger, A. T., Kuriyan, J. & Karplus, M. (1987) Crystallographic R ‐factor refinement by molecular dynamics, Science 35, 458–460.
7. Cohen‐Addad, C., Pares, S., Sicker, L., Neuburger, M. & Douce, R. (1995) The lipoamide arm in the glycine decarboxylase complex is not freely swinging, Nature Struct. Biol. 2, 63–68.
8. Dardel, F., Packman, L. C. & Perham, R. N. (1990) Expression in Escherichia coli of a sub‐gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of Bacillus stearothermophilus, FEBS Lett. 264, 206–210.
9. Dardel, F., Laue, E. D. & Perham, R. N. (1991) Sequence‐specific 1 H‐NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex, Eur. J. Biochem. 201, 203–209.
10. Dardel, F., Davis, A. L., Laue, E. D. & Perham, R. N. (1993) The three‐dimensional structure of the lipoyl domain from Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex, J. Mol. Biol. 229, 1037–1048.
11. Douce, R., Bourguignon, J., Macherel, D. & Neuburger, M. (1994) The glycine decarboxylase system in higher plant mitochondria: structure, function and biogenesis, Biochem. Soc. Trans. 22, 184–188.
12. Fujiwara, K., Okamura‐Ikeda, K. & Motokawa, Y. (1992) Expression of mature bovine H‐protein of the glycine cleavage system in Escherichia coli and in vitro lipoylation of the apoform, J. Biol. Chem. 267, 20011–20016.
13. Fujiwara, K., Okamura‐Ikeda, K. & Motokawa, Y. (1994) Purification and characterization of lipoyl‐AMP:N ε ‐lysine lipoyltransferase from bovine liver mitochondria, J. Biol. Chem. 269, 16605–16609.
14. Griffin, T. A., Winn, R. M. & Chuang, D. T. (1990) Expression and assembly of mature apotransacylase (E 2b) of bovine branched‐chain α‐keto acid dehydrogenase complex in Escherichia coli, J. Biol. Chem. 265, 12104–12110.
15. Kozak, M. (1983) Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles, Microbiol. Rev. 47, 1–45.
16. Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T 4, Nature 227, 680–685.
17. Macherel, D., Lebrun, M., Gagnon, J., Neuburger, M. & Douce, R. (1990) cDNA cloning, primary structure and gene expression for H protein, a component of the glycine‐cleavage system (glycine decarboxylase) of pea (Pisum sativum) leaf mitochondria, Biochem. J. 268, 783–789.
18. Macherel, D., Bourguignon, J. & Douce, R. (1992) Cloning of the gene (gdcH) encoding H‐protein, a component of the glycine decarboxylase complex of pea (Pisum sativum L.), Biochem. J. 286, 627–630.
19. Mattevi, A., De Kok, A. & Perham, R. N. (1992) The pyruvate dehydrogenase multienzyme complex, Curr. Opin. Struct. Biol. 2, 877–887.
20. Morris, T. W., Reed, K. E. & Cronan, J. E. (1994) Identification of the gene encoding lipoate‐protein ligase A of Escherichia coli, J. Biol. Chem. 269, 16091–16100.
21. Morris, T. W., Reed, K. E. & Cronan, J. E. (1995) Lipoic acid metabolism in Escherichia coli: The lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein, J. Bacteriol. 177, 1–10.
22. Neuburger, M., Jourdain, A. & Douce, R. (1991) Isolation of H protein loaded with methylamine as a transient species in glycine decarboxylase reactions, Biochem. J. 278, 765–769.
23. Pares, S., Cohen‐Addad, C., Sieker, L., Neuburger, M. & Douce, R. (1994) X‐ray structure determination at 2.6 Å resolution of a lipoate‐containing protein: the H protein of the glycine decarboxylase complex from pea leaves, Proc. Natl Acad. Sci. USA 91, 4850–4853.
24. Parry, R. J. (1983) Biosynthesis of some sulfur‐containing natural products. Investigations of the mechanism of carbon‐sulfur bond formation, Tetrahedron 39, 1215–1238.
25. Quinn, K., Diamond, A. G., Masters, A. K., Brookfield, D. E., Wallis, N. G. & Yeaman, S. J. (1993) Expression and lipoylation in Escherichia coli of the inner lipoyl domain of the E2 component of the human pyruvate dehydrogenase complex, Biochem. J. 289, 81–85.
26. Reed, K. E. & Cronan, J. E. (1993) Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes, J. Bacteriol. 175, 1325–1336.
27. Reed, K. E., Morris, T. M. & Cronan, J. E. (1994) Mutants of Escherichia coli K‐12 that are resistant to a selenium analog of lipoic acid identify unknown genes in lipoate metabolism, Proc. Natl Acad. Sci. USA 91, 3720–3724.
28. Reed, L. J., Koike, M., Levitch, M. E. & Leach, F. R. (1958a) Studies on the nature and reactions of protein‐bound lipoic acid, J. Biol. Chem. 232, 143–158.
29. Reed, L. J., Leach, F. R. & Koike, M. (1958b) Studies on a lipoic acid‐activating system, J. Biol. Chem. 232, 123–142.
30. Sieker, L., Cohen‐Addad, C., Neuburger, M. & Douce, R. (1991) Crystallographic data for H protein from the glycine decarboxylase complex, J. Mol. Biol. 220, 223–224.
31. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes, Methods Enzymol. 185, 60–89.
32. Walker, J. L. & Oliver, D. J. (1986) Glycine decarboxylase multienzyme complex, purification and partial characterization from pea leaf mitochondria, J. Biol. Chem. 261, 2214–2221.
33. White, R. H. (1980) Stable isotope studies on the biosynthesis of lipoic acid in Escherichia coli, Biochemistry 19, 15–19.