CYP2D6 mRNA expression in circulating peripheral blood mononuclear cells correlates with in vivo debrisoquine hydroxylase activity in extensive metabolizers

Research Communications in Molecular Pathology and Pharmacology

Volumn 91, Issue 2, 1996, Pages 149-159

  Carcillo, Joseph A ^a,b   Parise, Robert A ^b   Adedoyin, Adedayo ^b   Frye, Reginald ^b   Branches, Robert A ^b   Romkes, Marjorie ^b,c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH GRADUATE SCHOOL OF PUBLIC HEALTH   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; DEBRISOQUINE; DEBRISOQUINE 4 HYDROXYLASE; MESSENGER RNA;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DRUG HYDROXYLATION; DRUG MECHANISM; DRUG METABOLISM; ENZYME ACTIVATION; ENZYME ISOLATION; ENZYME SPECIFICITY; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; MONONUCLEAR CELL; ORAL DRUG ADMINISTRATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

ACTINS; ADULT; AGED; CYTOCHROME P-450 CYP2D6; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENOTYPE; HUMANS; MALE; MIDDLE AGED; MONOCYTES; NEOPLASMS; PHENOTYPE; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0029915050     PISSN: 00345164     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Agundez, J.A.G., Ledesma, M.C., Ladero, J.M., Benitez, J. (1995). Prevalence of CYP2D6 gene duplication and its repercussion of the oxidative phenotype in a white population. Clin. Pharmacol. Ther. 57, 265-269.
2. Cappraso, N.E., Shields, P.G., Landi, M.T., Shaw, G.L., Tucker, M.A., Hoover, R., Sugimura, H., Weston, A., Harris, C.C. (1992). The debrisoquine metabolic phenotype and DNA-based assays: implications of misclassification for the association of lung cancer and the debrisoquine metabolic phenotype. Environ. Hlth. Perspect. 98, 101-105.
3. Carcillo, J.A., Panse, R.A., Romkes-Sparks, M. (1994). Comparison of the enzyme-linked oligonucleotide sorbent assay to the 32P-labeled PCR/Southern blotting technique in quantitative analysis of human and rat mRNA. PCR Methods and Applications 3, 292-297.
4. Cosma, G., Taioli, E., Crofts, F., Toniolo, P., Garte, S. (1993). Genetic polymorphisms of the human CYP1A1 gene. Cancer Detection and Prevention 17, 137.
5. Fleming, C.M., Kaisary, A., Wilkinson, G.R., Smith, P., Branch, R.A. (1992). The ability to 4-hydroxylate debrisoquine is related to recurrence of bladder cancer. Pharmacogenetics 2, 128-134.
6. Fox, S.D., Shaw, G.L., Caporaso, N.E., Welsh, S.E., Mellini, D.W., Falk, R., Issaq, H.J. (1993). J. Liq. Chromatogr. 16, 1315-1327.
7. Gonzalez, F.J., Skoda, R.C., Kimura, S. Umeno, M., Zanger, U.M., Nebert, D.W., Gelboin, H.V., Hardwick, J.P., Meyer, U.A. (1988). Characterization of the common genetic defect in humans deficient in debrisoquine metabolism. Nature 331, 442-446.
8. Gough, A.C., Miles, J.S., Spurr, N.K. Moss, J. E., Gaedigk, A., Eichelbaum, M., Wolf, C. R. (1990). Identification of the primary gene defect at the cytochrome P4502D locus. Nature 347, 773-6.
9. Hanioka, N., Kimura, S., Meyer, U.A., Gonzalez, F.J. (1990). The human CYP2D locus associated with a common genetic defect in drug oxidation: a G(1934) to A base change in intron 3 of a mutant CYP2D6 allele results in an aberrant 3′ splice recognition site. Am. J. Hum. Genet. 47, 994-1001.
10. Heim, M., Meyer, U.A. (1990). Genotyping of poor metabolizers of debrisoquine by allele-specific PCR amplification. Lancet 336, 529-532.
11. Kaisary, A., Smith, P., Jacqz, E., McAllister, C.B., Wilkinson, G.R., Ray, W.A., Branch, R.A. (1987). Genetic predisposition to bladder cancer: ability to hydroxylate debrisoquine and mephenytoin as risk factors. Cancer Res. 47, 5488-5493.
12. Landi, M.T., Bertazzi, P.A., Shields, P.O., Clark, G., Lucier, G.W., Garte, S.J., Cosma, G., Caporaso, N. E. (1994). Association between CYP1A1 genotype, mRNA expression and enzymatic activity in humans. Pharmacogenetics 4, 242-246.
13. Nebert, D.W. (1991). Polymorphism of human CYP2D genes involved in drug metabolism: possible relationship to individual cancer risk. Cancer Cells 3, 93-96.
14. Nelson, D.R., Kamataki, T., Waxman, D.J., Guengerich, F.P., Estabrook, R.W., Feyereisen, R., Gonzalez, F.J., Coon, M.J., Gunsulas, I.C., Gotoh, O., Okuda, K., Nebert, D.W. (1993). The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature. DNA and Cell Biol 12, 1-51.
15. Omiecinski, C.J., Redlich, C.A., Costa, P. (1990). Induction and developmental expression of cytochrome P4501 A1 messenger RNA in rat and human tissues: detection by the polymerase chain reaction. Cancer Res. 50, 4315-4321.
16. Persad, R.A, Fleming, C.M., Wilkinson, G.R., Smith, P.J., Branch, R.A. (1992). Bladder cancer recurrence and its association with cytochrome P450IID6 activity. Prog. Clin. Biol. Res. 378. 19-27.
17. Redlich, C.A., Juilifs, D.M., Omiecinski, C.J. (1989). Genetic expression of the debrisoquine 4-hydroxylase polymorphism in human lymphocytes. Clin Res. 37:341 A.
18. Romkes-Sparks, M., Mnuskin, A., Chern, H.D., Persad, R., Fleming, C., Sibley, G., Smith, P., Wilkinson, G.R., Branch, R.A. (1994). Correlation of polymorphic expression of CYP2D6 mRNA in bladder mucosa and tumor tissue to in vivo debrisoquine hydroxylase activity. Carcinogenesis 15, 1955-1961.
19. Smith, C.A.D., Moss, J.E., Gough, A.C., Spurr, N.K., Wolf, C.R. (1992). Molecular genetic analysis of the cytochrome P450-debrisoquine hydroxylase locus and association with cancer susceptibility. Environ. Hlth. Perspect. 98, 107-112.
20. Vanden Heuvel, J.P., Clark, G.C., Thompson, C.L., McCoy, Z., Miller, C.R., Lucier, G.W., Bell, D. A. (1993). CYP1A1 mRNA levels as a human exposure biomarker: use of quantitative polymerase chain reaction to measure CYP1A1 expression in human peripheral blood lymphocytes. Carcinogenesis 14:2003-2006.
21. Wolf, C.R., Smith, C.A.D., Gough, A.C., Moss, I.E., Vallis, K.A., Howard, G., Carey, F.J., Mills, K., McNee, W., Carmichael, J., Spurr, N.K. (1992). Relationship between the debrisoquine hydroxylase polymorphism and cancer susceptibility. Carcinogenesis 13, 1035-1038.