Diminished microtubule-associated protein 2 (MAP2) immunoreactivity following cortical impact brain injury

Journal of Neurotrauma

Volumn 13, Issue 3, 1996, Pages 125-137

  Posmantur, R M ^a   Kampfl, A ^a   Taft, W C ^a   Bhattacharjee, M ^a   Dixon, C E ^a   Bao, J ^a   Hayes, R L ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

dendritic change; immunocytochemistry; microtubule associated protein 2; rat; Western blotting

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN 2;

ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN INJURY; CONTROLLED STUDY; CONTUSION; DENDRITIC CELL; IMMUNOCOMPETENT CELL; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MICROTUBULE ASSEMBLY; NONHUMAN; PATHOPHYSIOLOGY; PROTEIN DETERMINATION; RAT;

EID: 0029915040     PISSN: 08977151     EISSN: None     Source Type: Journal    
DOI: 10.1089/neu.1996.13.125     Document Type: Article

References (60)

1. ADAMS, J.H., SCOTT, G., PARKER, L.S., GRAHAM, D.I., and DOYLE, D. (1980). The contusion Index: A quantitative approach to cerebral contusions in head injury. Neuropathol. Appl. Neurobiol. 6, 319-324.
2. ARAI, A., KESSLER, M., LEE, K., and LYNCH, G. (1990). Calpain inhibitors improve the recovery of synaptic transmission from hypoxia in hippocampal slices. Brain Res. 532, 63-68.
3. ARAI, A., VANDERKLISH, P., KESSLER, M., LEE, K., and LYNCH, G. (1991). A brief period of hypoxia causes proteolysis of cytoskeletal proteins in hippocampal slices. Brain Res. 555, 276-280.
4. BARINAGA, M. (1995). Dendrites shed their dull image. Science 268, 200-201.
5. BERKOWITZ, S.A., KATAGIRI. L, BINDER, H.K., and WILLIAMS, R.C. (1977). Separation and characterization of microtubule proteins from calf brain. Biochemistry 16, 5610-5617.
6. BINDER, L.I., FRANKFURTER, A., and REBHUN, L.I. (1986). Differential localization of MAP2 and tau in mammalian neurons in situ. Ann. N.Y. Acad. Sci. 466, 145-166.
7. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 238-259.
8. BRYAN, R.M., CHERIAN, L., and ROBERTSON, C. (1995). Regional cerebral blood flow after controlled cortical impact in rats. Anesth. Analg. 80, 687-695.
9. COLLINS, R.C. (1986). Selective vulnerability of brain: New insights from the excitatory synapse. Metab. Brain Dis. 1(4), 231-240.
10. CROALL, D.E., and DEMARTINO, G.N. (1990). Regulation of calcium-dependent protease activity in vitro: Clues foe determining their physiological functions, in: CRC's Intracellular Calcium Dependent Proteolysis. R.L. Mellegren. and T. Murachi (eds). pps. 103-114.
11. DASH, P.K., MOORE, A.N., and DIXON, C.E. (1995). Spatial memory deficits, increased phosphorylation of the transcription factor CREB and induction of the AP-1 complex following experimental brain injury. J. Neurosci. 15, 2030-2039.
12. DIXON, C.E., LYETH, B.G., GIEBEL, M.L., et al. (1987). A fluid percussion model of experimental brain injury in the rat. J. Neurosurg. 67, 110-119.
13. DIXON, C.E., CLIFTON, G.L., LIGHTHALL, J.W., YAGHMAI, A.A., and HAYES, R.L. (1991). A controlled conical impact model of traumatic brain injury in the rat. J. Neurosci. Methods 39, 253-262.
14. EISENBERG, M., GARY, M.D., ALDRICH, S., et al. (1990). Initial CT findings in 753 patients with severe head injury. J. Neurosurg. 73, 688-698.
15. GOODMAN, J.C., CHERIAN. L., BRYAN, R.M., and ROBERTSON, C.S. (1994). Lateral conical impact injury in rats: Pathological effects of varying cortical compression and impact velocity. J. Neurotrauma 11, 587-597.
16. GRAHAM, D.I. (1992). Hypoxia and vascular disorders, in: Greenfield's Neuropathology. J. H. Adams and L.W. Duchen (eds), Oxford University Press: New York, pps. 153-269.
17. 45Ca autoradiographic study. Brain Res. 624, 94-102.
18. HAMM, R.J., DIXON, C.E., GBADEBO, D.M., et al. (1992). Cognitive deficits following traumatic brain injury produced by controlled cortical impact. J. Neurotrauma 9, 11-20.
19. HAYES, R.L. (1995). Neurochemical changes in traumatic brain injury, in: Catastrophic Brain Injury. H.S. Levin (ed). Oxford University Press: New York (in press).
20. HAYES, R.L., JENKINS, L.W., LYETH, B.G., et al. (1988). Pretreatment with phencyclidine, an N-methyl-D-aspartate antagonist, attenuates long-term behavioral deficits in the rat produced by traumatic brain injury. J. Neurotrauma 5, 259-274.
21. HAYES, R.L., JENKINS, L.W., and LYETH, E.G. (1992). Neuropharmacological mechanisms of traumatic brain injury: Acetylcholine and excitatory amino acids. J. Neurotrauma 9 (Suppl. 1), S173-S187.
22. HICKS, R.R., SMITH, D.H., and McINTOSH, T.K. (1995). Temporal response and effects of excitatory amino acid antagonism on microtubule-associated protein 2 immunoreactivity following experimental brain injury in rats. Brain Res. 678, 151-160.
23. INUZUKA, T., TAMURA, A., SATO, S., KIRINO, T., TOYOSHIMA, I., and MIYATAKE, T. (1990a). Suppressive effect of E-64c on ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. Brain Res. 526, 177-179.
24. INUZUKA, T., TAMURA, A., SATO, S., et al. (1990b). Changes in the concentrations of cerebral proteins following occlusion of the middle cerebral artery in rats. Stroke 21, 917-922.
25. ISOKAWA, M., and LEVESQUE, M.F. (1991). Increased NMDA responses and dendritic degeneration in human epileptic neurons in slices. Neurosci. Lett. 132, 212-216.
26. KAMPFL, A.W., WHITSON, J.S., ZHAO, X., DIXON, C.E., YANG, K., and HAYES, R.L. (1994). Short term potassium depolarization transiently decreases neurofilament proteins in mixed septo-hippocampal cultures. Soc. Neurosci. Abstr. 20, 179.7.
27. KAMPFL, A., ZHAO, X., WHITSON, J.S., et al. (1995a). Calpain inhibition protect against depolarization induced neurofilament protein loss of septo-hippocampal neurons in culture. Eur. J. Neurosci. (in press).
28. KAMPFL, A., WHITSON, S.J., ZHAO, X., POSMANTUR, R., CLIFTON, G.L., and HAYES, R.L. (1995b). Calpain inhibitors reduce depolarization induced loss of tau protein in primary septo-hippocampal cultures. Neurosci. Lett. 194, 149-152.
29. KITAGAWA, K., MATSUMOTO, M., NIINOBE, M., et al. (1989). Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-immunohistochemical investigation of dendritic damage. Neuroscience 2, 401-411.
30. LEE, K.S., FRANK, S., VANDERKLISH, P., ARAI, A., and LYNCH, G. (1991). Inhibition of proteolysis protects hippocampal neurons from ischemia. Proc. Natl. Acad. Sci. U.S.A. 88, 7233-7237.
31. LEWIS, S.A., VILLASANTE, A., SHERLINE, P., and COWAN, N. (1986). Brain specific expression of MAP2 detected using a cloned cDNA probe. J. Cell Biol. 102, 2098-2105.
32. LI, Z., HOGAN, E.L., and BANIK, N.L. (1995). Role of calpain in spinal cord injury: Increased mCalpain immunoreactivity in spinal cord after compression injury in the rat. Neurochem. Int. (in press).
33. LYETH, B.G., JENKINS, L.W., HAMM, R.J., et al. (1990). Prolonged memory impairment in the absence of hippocampal cell death following traumatic brain injury in the rat. Brain Res. 526, 249-258.
34. McINTOSH, T.K. (1993). Novel pharmacological therapies in the treatment of experimental traumatic brain injury: A review. J. Neurotrauma 10, 215-261.
35. McINTOSH, T.K., VINK, R., NOBLE, L., YAMAKAMI, S., SOARES, H., and FADEN, A. (1989). Traumatic brain injury in the cat: Characterization of a lateral fluid-percussion model. Neuroscience 28, 233-244.
36. MELLOR, G.W., SREEDHARAN, S.K., KOWLESSUR, D., THOMAS, E.W., and BROCKLEHURST. K. (1989). Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: Evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group. Biochem. J. 290, 75-83.
37. MILLER, L.P., LYETH, B.G., JENKINS, L.W., et al. (1990). Excitatory amino acid receptor subtype binding following traumatic brain injury. Brain Res. 526, 103-107.
38. MIYAZAKI, S., KATAYAMA, Y., LYETH, B.G., et al. (1992). Enduring suppression of hippocampal long-term potentiation following traumatic brain injury in rat. Brain Res. 585, 335-339.
39. MULTANI, P., MYERS, R.H., BLUME, H.W., SCHOMER, D.L., and SOTREL, A. (1994). Neocortical dendritic pathology in human partial epilepsy: A quantitative Golgi study. Epilepsia 35, 728-736.
40. NORTH, M.J. (1989). Prevention of unwanted proteolysis, Brenynon RJ and Bond in: Proteolytic Enzymes: A Practical Approach, R.J. Brenyon and J.S. Bond (eds), Oxford University Press: New York, 105-124.
41. POSMANTUR, R.M., HAYES, R.L., DIXON, C.E., and TAFT, W.C. (1994). Neurofilament 68 and neurofilament 200 protein levels decrease after traumatic brain injury. J. Neurotrauma 11, 533-545.
42. POSMANTUR, R.M., KAMPFL, A., HECK, K., et al. (1995a). Cytoskeletal derangements of cortical neuronal processes following traumatic brain injury in rats: An immunofluorescence study. J. Neuropathol. Exp. Neurol. 55, 68-80.
43. POSMANTUR, R.M., KAMPFL, A., SIMAN, R., LIU, S.J., CLIFTON, G.L., and HAYES, R.L. (1995b). A calpain inhibitor attenuates cortical cytoskeletal protein loss after experimental traumatic brain injury in the rat. J. Neurosci. (submitted).
44. POVLISHOCK, J.T. (1993). Pathobiology of traumatically induced axonal injury in animals and man. Ann. Emerg. Med. 22, 980-986.
45. REGEHR, W.G., and TANK, D.W. (1994). Dendritic calcium dynamics. Curr. Opin. Neurobiol. 4, 373-382.
46. ROBERTS-LEWIS, J.M., SAVAGE, M.J., MARCY, V.R., PINSKER, L., and SIMAN, R. (1994). Immunolocalization of Calpain 1-mediated spectrin degradation to vulnerable neurons in the ischemic gerbil brain. J. Neurosci. 14, 3934-3944.
47. SAATMAN, K.E., BOZYCZKO-COYNE, D., SIMAN, R., MARCY, V., GENNARELLI, T.A., and McINTOSH, T.K. (1994). Calpain activation following experimental brain injury. J. Neurotrauma Abstract (in press).
48. SCHORMAIR, C., BINGMANN, D., WITTKOWSKI, W., and SPECKMANN, E.J. (1993). Morphology of CA3 neurons in hippocampal slices with nonepileptic and epileptic activity: A light and electron microscopic study. Brain Res. Bull. 32, 329-338.
49. SIMAN, R., and NOSZEK, J.C. (1988). Excitatory amino acids activate calpain 1 and induce structural protein breakdown in vivo. Neuron 1, 279-287.
50. SIMAN, R., BAUDRY, M., and LYNCH, G. (1984). Brain fodrin: Substrate for calpain 1. an endogenous calcium-activated protease. Proc. Natl. Acad. Sci. U.S.A. 81, 3572-3576.
51. SIMAN, R., NOSZEK, J.C., and KEGERISE, C. (1989). Calpain 1 activation is specifically related to excitatory amino acid induction of hippocampal damage. J. Neurosci. 9, 1579-1590.
52. SUTTON, R.L., LESCAUDRON, L., and STEIN, D.G. (1993). Unilateral cortical contusion injury in the rat: Vascular disruption and temporal development of cortical necrosis. J. Neurotrauma 10, 135-149.
53. TAFT, W.C., YANG, K., DIXON, C.E., and HAYES, R.L. (1992). Microtubule-associated protein 2 levels decrease in hippocampus following traumatic brain injury. J. Neurotrauma 9, 281-290.
54. TAFT, W.C., YANG, K., DIXON, C.E., CLIFTON. G.L., and HAYES, R.L. (1993). Hypothermia attentuates the loss of hippocampal microtubule-associated protein 2 (MAP2) following traumatic brain injury. J. Cereb. Blood Flow Metab. 13, 796-802.
55. TOWBIN, H., STAEHELIN, T., and GORDON, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354.
56. VALDIVIA, M.M., AVILA, J., COLL, J., COLACO, C., and SANDOVAL, I.V. (1982). Quantitation and characterization of the microtubule-associated MAP2 in porcine tissues and its isolation from porcine (PK15) and human (HeLa) cell lines. Biochem. Biophys. Res. Commun. 105, 1241-1249.
57. WHITSON, J.S., KAMPFL, A.W., ZHAO, X., DIXON, C.E., and HAYES, R. (1995a). Brief potassium depolarization decreases neurofilament proteins in CNS culture. Brain Res. 948, 213-222.
58. WHITSON, J.S., KAMPFL, A., ZHAO, X., and HAYES, R.L. (1995b). Time course of neurofilament protein loss following depolarization-induced injury in CNS culture. Neurosci. Lett. 197, 159-163.
59. YAGHMAI, A., and POVLISHOCK, J.T. (1992). Traumatically induced reactive change as visualized through the use of monoclonal antibodies targeted to neurofilament subunits. J. Neuropathol. Exp. Neurol. 51, 158-176.
60. YUSTE, R., GUTNICK, M.J., SAAR, D., DELANEY, K.R., and TANK, D.W. (1994). Calcium accumulations in dendrites of neocortical pyramidal neurons: an apical band and evidence for two functional compartments. Neuron 13, 23-43.