Epidermal growth factor behaves as a partial agonist in hepatocytes: Effects on DNA synthesis in primary culture and competition with transforming growth factor α

Growth Factors

Volumn 13, Issue 3-4, 1996, Pages 171-179

  Guren, Tormod K ^a   Thoresen, G Hege ^a   Dajani, Olav F ^a   Taraldsrud, Eli ^a   Moberg, Enrica R ^a   Christoffersen, Thoralf ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

DNA synthesis; EGF; Hepatocytes; TFG

Indexed keywords

EPIDERMAL GROWTH FACTOR; TRANSFORMING GROWTH FACTOR ALPHA;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL CYCLE S PHASE; CONTROLLED STUDY; DNA SYNTHESIS; DOSE TIME EFFECT RELATION; DRUG POTENCY; LIVER CELL; LIVER CELL CULTURE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0029914718     PISSN: 08977194     EISSN: None     Source Type: Journal    
DOI: 10.3109/08977199609003219     Document Type: Article

References (49)

1. Ariëns, E. J. (1954) Affinity and intrinsic activity in the theory of competitive inhibition. I. Problems and theory. Arch. Int. Pharmacodyn. 99, 32-49.
2. Berry, N. M. and Friend, D. S. (1969) High-yield preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43, 506-520.
3. Brenner, D. A., Koch, K. S. and Leffert, H. L. (1989) Transforming growth factor-α stimulates proto-oncogene c-jun expression and a mitogenic program in primary cultures of adult rat hepatocytes. DNA 8, 279-285.
4. Brønstad, G. O., Sand, T. -E. and Christoffersen, T. (1983) Bidirectional concentration-dependent effects of glucagon and dibutyryl cyclic AMP on DNA synthesis in cultured adult rat hepatocytes. Biochim. Biophys. Acta 763, 58-63.
5. Bucher, N. L. R. and Strain, A. J. (1992) Regulatory mechanisms in hepatic regeneration. In Wright's Liver and Biliary Disease, G. H. Millward-Sadler, R. Wright, and M. J. P. Arthur, eds, W. B. Saunders Company LTD, London, pp. 258-274.
6. Carpenter, G. and Cohen, S. (1990) Epidermal growth factor. J. Biol. Chem. 265, 7709-7712.
7. Carpenter, G. and Wahl, M. I. (1991) The epidermal growth factor family. In Handbook of Exp. Pharmacol. Vol 95/I: Peptide Growth Factors and Their Receptors I, M. B. Sporn and A. B. Roberts, eds, Springer-Verlag, Berlin, pp. 69-171.
8. Christoffersen, T., Refsnes, M., Brønstad, G. O., Østby, E., Huse, J., Haffner, F., Sand, T. -E., Hunt, N. H. and Sonne, O. (1984) Changes in hormone responsiveness and cyclic AMP metabolism in rat hepatocytes during primary culture and effects of supplementing the medium with insulin and dexamethasone. Eur. J. Biochem. 138, 217-226.
9. Ciardiello, F., Kim, N., Saeki, T., Dono, R., Persico, M. G., Plowman, G. D., Garrigues, J., Radke, S., Todaro, G. J. and Salomon, D. S. (1991) Differential expression of epidermal growth factor related proteins in human colorectal tumors. Proc. Natl Acad. Sci. USA. 88, 7792-6.
10. Decker, S. J. (1990) Epidermal growth factor and transforming growth factor-alpha induce differential processing of the epidermal growth factor receptor. Biochem. Biophys. Res. Commun. 166, 615-621.
11. Derynck, R. (1988) Transforming growth factor α. Cell 54, 593-595.
12. Ebner, R. and Derynck, R. (1991) Epidermal growth factor and transforming growth factor-α: differential intracellular routing and processing of ligand-receptor complexes. Cell. Regul. 2, 599-612.
13. Earp, H. S., Dawson, T. L., Li, X. and Yu, H. (1995) Heterodimerization and functional interaction between EGF receptor family members: A new signaling paradigm with implications for breast cancer research. Breast Cancer Research and Treatment 35, 115-132.
14. Fausto, N., Laird, A. D. and Webber, E. M. (1995) Role of growth factors and cytokines in hepatic regeneration. FASEB J. 9, 1527-1536.
15. French, A. R., Tadaki, D. K., Niyogi, S. K. and Lauffenburger, D. A. (1995) Intracellular trafficking of epidermal growth factor family ligands is directly influenced by the pH sensitivity of the receptor/ligand interaction. J. Biol. Chem. 270, 4334-4340.
16. Gladhaug, I. P. and Christoffersen, T. (1987) Kinetics of epidermal growth factor binding and processing in isolated intact rat hepatocytes. Dynamic externalization of receptors during ligand internalization. Eur. J. Biochem. 164, 267-275.
17. Gladhaug, I. P. and Christoffersen, T. (1988) Rapid constitutive internalization and externalization of epidermal growth factor receptors in isolated rat hepatocytes. J. Biol. Chem. 263, 12199-12203.
18. Gladhaug, I. P. and Christoffersen, T. (1989) n-Butyrate and dexamethasone synergistically modulate the surface expression of epidermal growth factor receptors in cultured rat hepatocytes. FEBS lett. 243, 21-24.
19. Gladhaug, I. P., Refsnes, M. and Christoffersen, T. (1992) Regulation of surface expression of high-affinity receptors for epidermal growth factor (EGF) in hepatocytes by hormones, differentiating agents, and phorbol ester. Dig. Dis. Sci. 37, 233-239.
20. Gladhaug, I. P., Refsnes, M., Sand, T. -E. and Christoffersen, T. (1988) Effects of butyrate on epidermal growth factor receptor binding, morphology, and DNA synthesis in cultured rat hepatocytes. Cancer Res. 48, 6560-6564.
21. Gohda, E., Yamasaki, T., Tsubouchi, H., Kurobe, M., Sakiyama, O., Aoki, H., Niidani, N., Shin, S., Hayashi, K., Hashimoto, S., Daikuhara, Y. and Yamamoto, I. (1990) Biological and immunological properties of human hepatocyte growth factor from plasma of patients with fulminant hepatic failure. Biochim. Biophys. Acta 1053, 21-26.
22. Groenen, L. C., Nice, E. C. and Burgess, A. W. (1994) Structure-function relationships for the EGF/TGFα family of mitogens. Growth Factors 11, 235-257.
23. Gruppuso, P. A., Mead, J. E. and Fausto, N. (1990) Transforming growth factor receptors in liver regeneration following partial hepatectomy in the rat. Cancer Res. 50, 1464-1469.
24. Hasegawa, K., Miyata, Y. and Carr, B. I. (1994) Glutamic acid potentiates hepatocyte response to mitogens in primary culture. J. Cell. Physiol. 158, 365-373.
25. Lemmon, M. A. and Schlessinger, J. (1994) Regulation of signal transduction diversity by receptor oligomerization Trends Biochem. Sci. 19, 459-463.
26. Lee, D. C., Fenton, S. E., Berkowitz, E. A. and Hissong, A. (1995) Transforming growth factor α: Expression, regulation, and biological activities. Pharmacol. Rev. 47, 51-85.
27. Lin, Q., Blaisdell, J., O'Keefe, E. and Earp. H. S. (1984) Insulin inhibits the glucocorticoid-mediated increase in hepatocyte EGF binding. J. Cell. Physiol 119, 267-272.
28. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
29. McGowan, J. A. and Bucher, N. L. R. (1983) Pyruvate promotion of DNA synthesis in serum-free primary cultures of adult rat hepatocytes. In Vitro 19, 159-166.
30. Mead, J. E., Braun, L., Martin, D. A. and Fausto, N. (1990) Induction of replicative competence ("priming") in normal liver. Cancer Res. 50, 7023-7030.
31. Mead, J. E. and Fausto, N. (1989) Transforming growth factor α may be a physiological regulator of liver regeneration by means of an autocrine mechanism. Proc. Natl Acad. Sci. USA 86,1558-1562.
32. Moriai, T., Kobrin, M. S., Hope, C., Speck, L. and Korc, M. (1994) A variant epidermal growth factor receptor exhibits altered type α transforming growth factor binding and transmembrane signaling. Proc. Natl Acad. Sci. USA 91, 10217-10221.
33. Ni, N. and Yager, J. D. (1994) Comitogenic effects of estrogens on DNA synthesis induced by various growth factors in cultured female rate hepatocytes. Hepatology 19, 183-192.
34. 2α: Additivity with the effect of norepinephrine, and synergism with epidermal growth factor. J. Cell. Physiol. 159, 35-40.
35. Richman, R. A., Claus, T. H., Pilkis, S. J. and Friedman, D. L. (1976) Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc. Natl Acad. Sci. USA 73, 3589-3593.
36. Ross, E. M. (1996) Mechanisms of drug action and the relationship between drug connection and effect. In: Goodman & Gilman's The Parmacological Basis of Therapeutics. 9th Ed., Hardman, J. G., Limbird, L. E., Molinoff, P. B., Ruddon, R. W., Gilman, A. G., eds., McGraw Hill, New York, pp. 29-41.
37. Sand, T.-E., Brønstad, G., Digernes, V., Killi, A., Amara, W., Refsnes, M. and Christoffersen, T. (1985) Quantitative aspects of the effects of insulin, epidermal growth factor and dexamethasone on DNA synthesis in cultured adult rat hepatocytes. Acta Endocrinol. 109, 369-377.
38. Sand, T.-E. and Christoffersen, T. (1987) Temporal requirement for epidermal growth factor and insulin in the stimulation of hepatocyte DNA synthesis. J. Cell. Physiol 131, 141-148.
39. Sand, T.-E. and Christoffersen, T. (1988) A simple medium for the study of hepatocyte growth in culture under defined conditions. In Vitro Cell. Dev. Biol. 24, 981-984.
40. Simpson, R. J., Smith, J. A., Moritz, R. L., O'Hare, M. J., Rudland, P. S., Morrison, J. R., Lloyd, C. J., Grego, B., Burgess, A. W. and Nice E. C. (1985) Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity. Eur. J. Biochem. 153, 629-637.
41. Smith, J. J., Derynck, R. and Korc, M. (1987) Production of transforming growth factor α in human pancreatic cancer cells: Evidence for a superagonist autocrine cycle. Proc. Natl Acad. Scui. USA 84, 7567-7570.
42. Stephenson, R. P. (1956) A modification of receptor theory. Br. J. Pharmacol. 11, 379-393.
43. Strömblad, S. and Andersson, G. (1993) The coupling between transforming growth factor-α and the epidermal growth factor receptor during rat liver regeneration. Exp. Cell Res. 204, 321-328.
44. Strömblad, S., Eriksson, L. C. and Andersson, G. (1994) Increased expression of and sensitivity of transforming growth factor-α: A promotive role during rat liver carcinogenesis. Mol. Carcinog. 10, 97-104.
45. Theocharis, S. E., Margeli, A. P. and Tsokos, M. G. (1995) Alpha-2b-interferon inhibits rat liver regeneration after partial hepatectomy without affecting thymidine kinase Activity. J. Lab. Clin. Med. 125, 588-596.
46. Ullrich, A. and Schlessinger, J. (1990) Signal transduction by receptors with tyrosine kinase activity. Cell 61, 203-212.
47. van der Geer, P., Hunter, T. and Lindberg, R. A. (1994) Receptor protein-tyrosine kinases and their signal transduction pathways. Annu. Rev. Cell Biol. 10, 251-337.
48. Winkler, M. E., O'Connor, L., Winget, M. and Fendly, B. (1989) Epidermal growth factor and transforming growth factor α bind differently to the epidermal growth factor receptor. Biochemistry 28, 6373-6378.
49. Wollenberg, G. K., Harris, L., Farber, E. and Hayes, M. A. (1989) Inverse relationship between epidermal growth factor induced proliferation and expression of high affinity surface epidermal growth factor receptors in rat hepatocytes. Lab. Invest. 60, 254-259.