Pyruvate reduces anoxic injury and free radical formation in perfused rat hepatocytes

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 270, Issue 3 33-3, 1996, Pages

  Borle, André B ^a   Stanko, Ronald T ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

anoxia; antimycin A; hydrogen peroxide; lactate dehydrogenase; reoxygenation; superoxides

Indexed keywords

ANTIMYCIN A1; FREE RADICAL; HYDROGEN PEROXIDE; LACTATE DEHYDROGENASE; PYRUVIC ACID; SUPEROXIDE;

ANIMAL CELL; ANOXIA; ANTIOXIDANT ACTIVITY; ARTICLE; CHEMOLUMINESCENCE; CONTROLLED STUDY; ENZYME RELEASE; LIVER CELL; LIVER MITOCHONDRION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; REOXYGENATION; REPERFUSION INJURY;

EID: 0029913422     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1996.270.3.g535     Document Type: Article

References (29)

1. Andrae, U., J. Singh, and K. Ziegler-Skylakakis. Pyruvate and related α-ketoacids protect mammalian cells in culture against hydrogen peroxide-induced cytotoxicity. Toxicol. Lett. 28: 93-98, 1985.
2. Borle, A. B., and K. W. Snowdowne. Measurement of intracellular ionized calcium with aequorin. Methods Enzymol. 124: 90-116, 1986.
3. Bunger, R., R. T. Mallet, and D. A. Hartman. Pyruvate-enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart. Near-complete prevention of reperfusion contractile failure. Eur. J. Biochem. 180: 221-233, 1989.
4. Caraceni, P., A. Gasbarrini, D. H. Van Thiel, and A. B. Borle. Oxygen free radical formation by isolated hepatocytes during postischemic reoxygenation: scavenging effect of albumin. Am. J. Physiol. 266 (Gastrointest. Liver Physiol. 29): G451-G458, 1994.
5. Caraceni, P., E. R. Rosenblum, D. H. Van Thiel, and A. B. Borle. Reoxygenation injury in isolated rat hepatocytes. Relation to oxygen free radicals and lipid peroxidation. Am. J. Physiol. 266 (Gastrointest. Liver Physiol. 29): G799-G806, 1994.
6. Caraceni, P., H. S. Ryu, D. H. Van Thiel, and A. B. Borle. Source of oxygen free radicals produced by rat hepatocytes during postanoxic reoxygenation. Biochim. Biophys. Acta 1268: 249-254, 1995.
7. Caraceni, P., D. H. Van Thiel, and A. B. Borle. Dual effect of deferoxamine on free radical formation and reoxygenation injury in isolated hepatocytes. Am J. Physiol. 269 (Gastrointest. Liver Physiol. 32) G132-G137, 1995.
8. Caraceni, P., T. Yao, A. Gasbarrini, M. Zern, A. B. Borle, S. Degli Esposi, and D. H. Van Thiel. Effect of vitamin E on reoxygenation injury experienced by isolated rat hepatocytes. Life Sci. 55: 1427-1432, 1994.
9. Cavallini, L., M. Valente, and M. P. Rigobello. The protective action of pyruvate on recovery of ischemic heart: comparison with other oxidizable substrates. J. Mol. Cell Cardiol. 22: 143-154, 1990.
10. Deboer, L. W. V., P. A. Bekx, L. Han, and L. Steinke. Pyruvate enhances recovery of hearts after ischemia and reperfusion by preventing free radical generation. Am. J. Physiol. 265 (Heart Circ. Physiol. 34): H1571-H1576, 1993.
11. - Free Rad. Biol. Med. 15: 447-451, 1993.
12. Göldlin, C. R., and U. A. Boelsterli. Reactive oxygen species and non-peroxidative mechanisms of cocain-induced cytotoxicity in rat hepatocyte cultures. Toxicology 69: 79-91, 1991.
13. Gutteridge, M. C., R. Richmond, and B. Halliwell. Inhibition of the iron-catalyzed formation of hydroxyl radicals from superoxide and of lipid peroxidation by desferrioxamine. Biochem. J. 184: 469-472, 1979.
14. Halestrap, A. P., R. D. Scott, and A. P. Thomas. Mitochondrial pyruvate transport and its hormonal regulation. Int. J. Biochem. 11: 97-105, 1980.
15. Halliwell, B. Protection against tissue damage in vivo by desferrioxamine: What is its mechanism of action? Free Rad. Biol. Med. 7: 645-651, 1989.
16. Hems, D. A., and J. T. Brosnan. Effect of ischaemia on content of metabolites in rat liver and kidney in vivo. Biochem. J. 120: 105-111, 1970.
17. 2-activated metmyoglobine and other peroxidizing systems. Free Rad. Biol. Med. 3: 309-317, 1987.
18. Klebanoff, S. J., and A. M. Waltersdorph. Inhibition of peroxidase-catalyzed reactions by deferoxamine. Arch. Biochem. Biophys. 264: 600-606, 1988.
19. Kowalski, D. P., T. Y. Aw, Y. Park, and D. P. Jones. Postanoxic oxidative injury in rat hepatocytes: lactate-dependent protection against tert-butylhydroperoxide. Free Rad. Biol. Med. 12: 205-212, 1992.
20. 2 in vitro and in vivo. Free Rad. Biol. Med. 16: 29-33, 1994.
21. Littauer, A., and H. de Groot. Release of reactive oxygen by hepatocytes on reoxygenation: three phases and role of mitochondria. Am. J. Physiol. 262 (Gastrointest. Liver Physiol. 25): G1015-G1020, 1992.
22. Murer, H., and G. Burckhardt. Membrane transport of anions across epithelia of mammalian small intestine and kidney proximal tubules. Rev. Physiol. Biochem. Pharmacol. 96: 1-51, 1983.
23. O'Donnell-Tormey, J., C. F. Nathan, K. Lanks, C. J. DeBoer, and J. de la Harpe. Secretion of pyruvate. An antioxidant defense of mammalian cells. J. Exp. Med. 165: 500-514, 1987.
24. 2+ depletion prevents anoxic death of hepatocytes by inhibiting mitochondrial permeability transition. Am. J. Physiol. 268 (Cell Physiol. 37): C676-C685, 1995.
25. Rembish, S. J., and M. A. Trush. Further evidence that lucigenin-derived chemiluminescence monitors mitochondrial superoxide generation in rat alveolar macrophages. Free Rad. Biol. Med. 17: 117-126, 1994.
26. Rigobello, M. P., and A. Bindoli. Effect of pyruvate on heart thiol status during ischemia and hypoxia followed by reperfusion. Mol. Cell. Biochem. 122: 93-100, 1993.
27. Salahudeen, A. K., E. C. Clark, and K. A. Nath. Hydrogen peroxide-induced renal injury. A protective role for pyruvate in vitro and in vivo. J. Clin. Invest 88: 1886-1893, 1991.
28. Van Bilsen, M., G. J. van der Vusse, L. H. E. H. Snoeckx, T. Arts, W. A. Coumans, P. H. M. Willemsen, and R. S. Reneman. Effect of pyruvate on post-ischemic myocardial recovery at various workloads. Pfluegers Arch. 413: 167-173, 1988.
29. Van Bilsen, M., G. J. van der Vusse, P. H. M. Willemsen, W. A. Coumans, and R. S. Reneman. Fatty acid accumulation during ischemia and reperfusion: effects of pyruvate and POCA, a carnitine palmitoyltransferase I inhibitor. J. Mol. Cell Cardiol. 23: 1437-1447, 1991.