Effect of antipeptide antibodies directed against three domains of connexin43 on the gap junctional permeability of cultured heart cells

Journal of Membrane Biology

Volumn 150, Issue 3, 1996, Pages 243-253

  Bastide, B ^a,d   Jarry Guichard, T ^b   Briand, J P ^c   Deleze J ^a   Gros, D ^b  

^a UNIVERSITÉ DE POITIERS   (France)

^b AIX MARSEILLE UNIVERSITÉ   (France)

^c INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^d UNIV LILLE   (France)

Author keywords

Antipeptide antibodies; Cardiac myocytes; Cell to cell dye transfer; Connexin43; Gap junction; Intercellular communication

Indexed keywords

CONNEXIN 43; GAP JUNCTION PROTEIN; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL JUNCTION; CONFORMATIONAL TRANSITION; HEART MUSCLE CELL; IMMUNOCYTOCHEMISTRY; NONHUMAN; PROTEIN CONFORMATION; RAT; STEREOSPECIFICITY;

ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; CELL COMMUNICATION; CELLS, CULTURED; CONNEXIN 43; GAP JUNCTIONS; MICROINJECTIONS; MYOCARDIUM; PEPTIDE FRAGMENTS; PERMEABILITY; RATS;

ANIMALIA;

EID: 0029913029     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002329900048     Document Type: Article

References (45)

1. Barrows, G.H., Sisken, J.E., Allegra, J.C., Grasch, S.D. 1984. Measurement of fluorescence using digital integration of video images. J. Histochem. Cytochem. 32:741-746
2. Bastide, B., Hervé, J.C., Cronier, L., Délèze, J. 1995. Rapid onset and calcium independence of the gap junction uncoupling induced by heptanol in cultured heart cells. Pfluegers Arch. 429:386-393
3. Bastide, B., Jarry-Guichard, T., Gros, D., Délèze, J. 1993. Effect of antibodies directed against three peptide sequences of connexin43 on gap junctional communication of heart cells in primary culture. J. Mol. Cell Cardiol. Suppl. 25 IV:S21
4. Bastide, B., Jarry-Guichard, T., Hervé, J.C., Gros, D., Délèze, J. 1995. Depressing effect of polyclonal antibodies directed against a carboxy-terminus domain of connexin43 on cell-to-cell dye-transfer in cultured heart myocytes. In: Intercellular Communication through Gap Junctions, Progress in Cell Research. Y. Kanno, K. Kataoka, Y. Shiba, Y. Shibata, T. Shimazu, editors. Vol. 4, pp. 159-162. Elsevier Science B.V., Amsterdam
5. Becker, D.L., Evans, W.H., Green, C.R., Warner, A. 1995. Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions. J. Cell Sci. 108:1455-1467
6. Bennett, M.V.L., Barrio, L.C., Bargiello, T.A., Spray, D.C., Hertzberg, K., Sáez, J.C. 1991. Gap junctions - new tools, new answers, new questions. Neuron 6:305-320
7. Beyer, E.C. 1993. Gap junctions. Int. Rev. Cytol. 137C:1-37
8. Beyer, E.C., Kistler, J., Paul, D.L., Goodenough, D.A. 1989. Antisera directed against connexin43 peptides react with a 43-kDa protein localized to gap junctions in myocardium and other tissues. J. Cell Biol. 108:545-605
9. Beyer, E.C., Paul, D.L., Goodenough, D.A. 1987. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol. 105:2621-2629
10. Beyer, E.C., Paul, D.L., Goodenough, D.A. 1990. Connexin family of gap junction proteins. J. Membrane Biol. 116:187-194
11. Briand, J.P., Müller, S., Van Regenmortel, M.H.V. 1986. Synthetic peptides as antigens: pitfalls of conjugation methods. J Immunol. Methods 78:59-69
12. Darrow, B.J., Laing, J.G., Lampe, P.D., Saffitz, J.E., Beyer, E.C. 1995. Expression of multiple connexins in cultured neonatal rat ventricular myocytes. Circ. Res. 76:381-387
13. Dupont, E., El Aoumari, A., Roustiau-Sévère, S., Briand, J.P., Gros, D. 1988. Immunological characterization of rat cardiac gap junctions: presence of common antigenic determinants in heart of other vertebrate species and in various organs. J. Membrane Biol. 104:119-128
14. El Aoumari, A., Fromaget, C., Dupont, E., Reggio, H., Durbec, P., Briand, J.P., Boller, K., Kreitmann, B., Gros, D. 1990. Conservation of a cytoplasmic carboxy-terminal domain of connexin-43, a gap junctional protein, in mammal heart and brain. J. Membrane Biol. 115:229-240
15. Giaume, C., Fromaget, C., El Aoumari, A., Cordier, J., Glowinski, J., Gros, D. 1991. Gap junctions in cultured astrocytes - single-channel currents and characterization of channel-forming protein. Neuron 6:133-143
16. Gros, D., Jarry-Guichard, T., Ten Velde, I., De Mazière, A., Van Kempen, M.J.A., Davoust, J., Briand, J.P., Moorman, A.F.M., Jongsma, H.J. 1994. Restricted distribution of connexin40, a gap junctional protein, in mammalian heart. Circ. Res. 74:839-851
17. Haefliger, J.-A., Bruzzone, R., Jenkins, N.A., Gilbert, D.J., Copeland, N.G., Paul, D.L. 1992. Four novel members of the connexin family of gap junction proteins - Molecular cloning, expression, and chromosome mapping. J. Biol. Chem. 267:2057-2064
18. Hertzberg, E.L., Spray, D.C., Bennett, M.V.L. 1985. Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide. Proc. Natl. Acad. Sci. USA 82:2412-2416
19. Kanter, H.L., Saffitz, J.E., Beyer, E.C. 1992. Cardiac myocytes express multiple gap junction proteins. Circ. Res. 70:438-444
20. Kennelly, P.J., Krebs, E.G. 1991. Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases. J. Biol. Chemistry 266:15555-15558
21. Kumar, N.M., Gilula, N.B. 1986. Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein. J. Cell Biol. 103:767-776
22. Laird, D.W., Puranam, K.L., Revel, J.-P. 1991. Turnover and phosphorylation dynamics of connexin43 gap junction protein in cultured cardiac myocytes. Biochem. J. 273:67-72
23. Laird, D.W., Revel, J.-P. 1990. Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes. J. Cell Sci. 97:109-117
24. Lal, R., Laird, D.W., Revel, J.-P. 1993. Antibody perturbation analysis of gap-junction permeability in rat cardiac myocytes. Pfluegers Arch. 422:449-457
25. Lau, A.F., Hatchpigott, V., Crow, D.S. 1991. Evidence that heart connexin43 is a phosphoprotein. J. Mol. Cell. Cardiol. 23:659-663
26. Lee, S., Gilula, N.B., Warner, A.E. 1987. Gap junctional communication and compaction during preimplantation stages of mouse development. Cell 51:851-860
27. Makowski, L., Caspar, D.L.D., Phillips, W.C., Goodenough, D.L. 1977. Gap junction structures II. Analysis of the X-ray diffraction data. J. Cell Biol. 74:629-645
28. Manjunath, C.K., Goings, G.E., Page, E. 1984. Cytoplasmic surface and intramembrane components of rat heart gap junctional proteins. Am. J. Physiol. 246:H865-H875
29. Merrifield, R. 1963. Solid-phase peptide synthesis. I: the synthesis of a tetrapeptide. J. Am. Chem. Soc. 85:2149-2154
30. Meyer, R.A., Laird, D.W., Revel, J.P., Johnson, R.G. 1992. Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies. J. Cell Biol. 119:179-189
31. Milks, L.C., Kumar, N.M., Houghten, R., Unwin, N., Gilula, N.B. 1988. Topology of the 32-kDa liver gap junction protein determined by site-directed antibody localizations. EMBO J. 7:2967-2975
32. Paul, D.L. 1986. Molecular cloning of cDNA for rat liver gap junction protein. J. Cell Biol. 103:123-134
33. Pluciennik, F., Joffre, M., Délèse, J. 1994. Follicle-stimulating hormone increases gap junction communication in Sertoli cells from immature rat testis in primary culture. J. Membrane Biol. 139:81-96
34. Rotman, B., Papermaster, B.W. 1966. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc. Natl. Acad. Sci. USA 55:134-141
35. Sáez, J.C., Berthoud, V.M., Moreno, A.P., Spray, D.C. 1993. Gap junctions. Multiplicity of controls in differentiated and undifferentiated cells and possible functional implications. In: Advances in Second Messengers and Phosphoprotein Research, S. Shenolikar, A.C. Nairn, editors. Vol. 27: pp. 163-198. Raven Press, New York
36. Sáez, J.S., Nairn, A.C., Czernik, A.J., Spray, D.C., Hertzberg, E.L. 1993. Rat connexin43: regulation by phosphorylation in heart. In: Progress in Cell Research, J.E. Hall, G.A. Zampighi, R.M. Davis, editors. Vol. 3: pp. 275-281. Elsevier Science, B.V. Amsterdam
37. Socolar, S.J., Loewenstein, W.R. 1979. Methods for studying transmission through permeable cell-to-cell junctions. In: Methods in Membrane Biology. D.E. Korn, editor. Vol. 10: pp. 123-179. Plenum, New York
38. Van Kempen, M.J.A., Fromaget, C., Gros, D., Moorman, A.F.M., Lamers, W.H. 1991. Spatial distribution of connexin43. the major cardiac gap junction protein, in the developing and adult rat heart. Circ. Res. 68:1638-1651
39. Van Kempen, M.J.A., Ten Velde, I., Wessels, A., Oosthoek, P.W., Gros, D., Jongsma H.J., Moorman, A.F.M., Lamers, W.H. 1995. Differential connexin distribution accommodates cardiac function in different species. Microsc. Res. Tech. 31:420-436
40. Wade, H.M., Trosko, E.J., Schindler, M. 1986. A fluorescence photo-bleaching assay of gap junction-mediated communication between human cells. Science 232:525-528
41. Warner, A.E., Guthrie, S.C., Gilula, N.B. 1984. Antibodies to gap-junctional protein selectively disrupt junctional communication in the early amphibian embryo. Nature 311:127-131
42. Willecke, K., Hennemann, H., Dahl, E., Jungbluth, S., Heynkes, R. 1991. The diversity of connexin genes encoding gap junctionul proteins - Minireview. Eur. J. Cell Biol. 56:1-7
43. Yancey, S.B., John, S.A., Lal, R., Austin, B.J., Revel, J.-P. 1989. The 43-kD polypeptide of heart gap junctions: immunolocalization (I), topology (II), and functional domains (III). J. Cell Biol 108:2241-2254
44. Yeager, M., Gilula, N.B. 1992. Membrane topology and quaternary structure of cardiac gap junction ion channels. J. Mol. Biol. 223:929-948
45. Zimmer, D.B., Green, C.R., Evans, W.H., Gilula, N.B. 1987. Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures. J. Biol. Chemistry 262:7751-7763