Suppression of temperature-sensitive defects of polypeptide release factors RF-1 and RF-2 by mutations or by an excess of RF-3 in Escherichia coll

Journal of Molecular Biology

Volumn 258, Issue 4, 1996, Pages 588-599

  Matsumura, Kiyoyuki ^a   Ito, Koichi ^a   Kawazu, Yoichi ^a   Mikuni, Osamu ^a,b   Nakamura, Yoshikazu ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b DNAVEC Research Inc   (Japan)

Author keywords

Release factor 1; Release factor 2; Release factor 3; Stop codon recognition; Suppression

Indexed keywords

BACTERIAL PROTEIN; MUTANT PROTEIN; TRANSLATION TERMINATION FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENE ISOLATION; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS REGULATION; STRUCTURE ACTIVITY RELATION; TEMPERATURE SENSITIVE MUTANT; TRANSLATION REGULATION;

EID: 0029912348     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0271     Document Type: Article

References (40)

1. Ævarsson, A., Brazhnikov, E., Garber, M., Zheltonosova, J., Chirgadze, Yu., Al-Karadaghi, S., Svensson, L. A. & Liljas, A. (1994). Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus. EMBO J. 13, 3669-3677.
2. Aiba, H. Adhya, S. & de Crombrugghe, B. (1981). Evidence for two functional gal promoters in intact Escherichia coli cells. J. Biol. Chem. 256, 11905-11910.
3. Berman, M. L. & Landy, A. (1979). Promoter mutations in the transfer RNA gene tyrT of Escherichia coli. Proc. Natl Acad. Sci. USA, 76, 4303-4307.
4. Bourne, H. R., Sanders, D. A. & McCormick, F. (1991). The GTPase superfamily: conserved structure and molecular mechanism. Nature, 349, 117-127.
5. Capecchi, M. R. & Klein, H. A. (1969). Characterization of three proteins involved in polypeptide chain termination. Cold Spring Harbor Symp. Quant. Biol. 34, 469-477.
6. Casadaban, M. J. & Cohen, S. N. (1980). Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138, 179-207.
7. Casadaban, M. J., Chou, J. & Cohen, S. N. (1980). In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J. Bacteriol. 143, 971-980.
8. Caskey T., Scolnick, E., Tompkins, R., Goldstein, J. & Milman, G. (1969). Peptide chain termination, codon, protein factor, and ribosomal requirements. Cold Spring Harbor Symp. Quant. Biol. 34, 479-488.
9. Caskey, C. T., Forrester, W. C., Tate, W. & Ward, C. D. (1984). Cloning of the Escherichia coli release factor 2 gene. J. Bacteriol. 158, 365-368.
10. Craigen, W. J., Lee, C. C. & Caskey, C. T. (1990). Recent advances in peptide chain termination. Mol. Microbiol. 4, 861-865.
11. Elliott, T. & Wang, X. (1991). Salmonella typhimurium prfA mutants defective in release factor 1. J. Bacteriol. 173, 4144-4154.
12. Goldstein, J. L. & Caskey, C. T. (1970). Peptide chain termination: effect of protein S on ribosomal binding of release factors. Proc. Natl Acad. Sci. USA, 67, 537-543.
13. Grentzmann, G., Brechemier-Baey D., Heurgue, V., Mora, L. & Buckingham, R. H. (1994). Localization and characterization of the gene encoding release factor RF3 in Escherichia coli. Proc. Natl Acad. Sci. USA, 91, 5848-5852.
14. Grentzmann, G., Brechemier-Baey D., Heurgué-Hamard, V. & Buckingham, R. H. (1995). Function of polypeptide chain release factor RF-3 in Escherichia coli. RF-3 action in termination is predominantly at UGA-containing stop signals. J. Biol. Chem. 270, 10595-10600.
15. Groisman, E. A. & Casadaban, M. J. (1986). Mini-Mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J. Bacteriol. 168, 357-364.
16. Ito, K., Kawakami, K. & Nakamura, Y. (1993). Multiple control of Escherichia coli lysyl-tRNA synthetase expression involves a transcriptional repressor and a translational enhancer element. Proc. Natl Acad. Sci. USA, 90, 302-306.
17. Kawakami, K. & Nakamura, Y. (1990). Autogenous suppression of an opal mutation in the gene encoding peptide chain release factor 2. Proc. Natl Acad. Sci. USA, 87, 8432-8436.
18. Kawakami, K., Inada, T. & Nakamura, Y (1988a). Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli. J. bacteriol. 170, 5378-5381.
19. Kawakami, K., Jönsson, Y. H., Björk, G. R., Ikeda, H. & Nakamura, Y. (1988b). Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli. Proc. Natl Acad. Sci. USA, 85, 5620-5624.
20. Kawazu, Y, Ito, K., Matsumura, K. & Nakamura, Y. (1995). Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium and Dichelobacter nodosus. J. Bacteriol. 177, 5547-5553.
21. α reporter gene of pUC8/9 and pUC18/19 plasmids. Gene, 68, 159-162.
22. Mead, D. A., Szczesna-Skorupa, E. & Kemper, B. (1986). Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1, 67-74.
23. Mikuni, O., Kawakami, K. & Nakamura, Y. (1991). Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli. Biochimie, 73, 1509-1516.
24. Mikuni, O., Ito, K., Moffat, J., Matsumura, K., McCaughan, K., Nobukuni, T., Tate, W. & Nakamura, Y. (1994). Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli. Proc. Natl Acad. Sci. USA, 91, 5798-5802.
25. Miller, J. (1972). Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
26. Nakamura, Y., Mizusawa, S., Court, D. L. & Tsugawa, A. (1986). Regulatory defects of a conditionally lethal nusAts mutant of Escherichia coli; positive and negative modulator roles of NusA protein in vivo. J. Mol. Biol. 189, 103-111.
27. Roesser, J. R., Nakamura, Y. & Yanofsky, C. (1989). Regulation of basal level expression of the tryptophane operon of Escherichia coli. J. Biol. Chem. 264, 12284-12288.
28. Rydén, S. M. & Isaksson, L. A. (1984). A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors. Mol. Gen. Genet. 193, 38-45.
29. Rydén, M., Murphy, J., Martin, R., Isaksson, L. & Gallant, J. (1986). Mapping and complementation studies of the gene for release factor 1. J. Bacteriol. 168, 1066-1069.
30. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-491.
31. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edit., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
33. Scolnick, E., Tompkins, R., Caskey T. & Nirenberg, M. (1968). Release factors differing in specificity for terminator codons. Proc. Natl Acad. Sci. USA, 61, 768-774.
34. Siebenlist, U., Simpson, R. B. & Gilbert, W. (1980). E. coli RNA polymerase interacts homologously with two different promoters. Cell, 20, 269-281.
35. Singer, M., Baker, T. A., Schnitzler, G., Deischel, S. M., Goel, M., Dove, W., Jaacks, K. J., Grossman, A. D., Erickson, J. W. & Gross, C. A. (1989). A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53, 1-24.
36. Tate, W. P. & Brown, C. M. (1992). Translational termination: "stop" for protein synthesis or "pause" for regulation of gene expression. Biochemistry, 31, 2443-2450.
37. Vieira, J. & Messing, J. (1987). Production of single-stranded plasmid DNA. Methods Enzymol 153, 3-11.
38. Way, J. C., Davis, M. A., Morisato, D., Roberts, D. E. & Kleckner, N. (1984). New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene, 32, 369-379.
39. Weiss, R. B., Murphy, J. P. & Gallant, J. A. (1984). Genetic screen for cloned release factor genes. J. Bacteriol. 158, 362-364.
40. Zhang, S., Rydén-Aulin, M., Kirsebom, L. A. & Isaksson, L. A. (1994). Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo. J. Mol. Biol. 242, 614-618.