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Studies of the topoisomerase II-mediated cleavage and religation reactions by use of a suicidal double-stranded DNA substrate
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Roca J, Wang JC: DNA transport by a type II DNA topoisomerase: evidence in favor of a two-gate mechanism. Cell 1994, 77:609-616. This paper details a set of experiments which use the AMPPNP-driven decatenation of a pair of singly interlocked duplex DNA rings to dissect the strand-passage reaction of a type II topoisomerase. The results provide strong evidence that the passed DNA duplex enters and exits the enzyme at different sites.
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