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HMBC shows also a strong correlation between C-26 (Hiv 1) and the multiplet at δ = 5.08-5.09 ppm. This multiplet is produced by the overlapping of the H-21 (Hmp 1) and H-27 (Hiv 1) resonances, and so, the connection between Hmp 1 and Hiv 1 could only be unambiguously established after MS and selective hydrolysis.
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10544256475
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note
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According to the literature, NOEs between H-16 and H-21 should be expected if the linkage Pro 1-Hmp 1 were cis and between H-19 and H-21 if trans. Models indicate that in 4 both NOEs are possible depending on the flexibility of the molecule and, in fact, we have observed them experimentally.
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25
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10544227618
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