Phosphoregulatory tyrosine of Xenopus mitogen-activated protein kinase is out of the reach of the enzyme catalytic center after autophosphorylation. Biochemical evidence for conformational changes upon phosphorylation

European Journal of Biochemistry

Volumn 241, Issue 2, 1996, Pages 322-329

  Tokmakov, Alexander A ^a   Sahara, Setsuko ^a   Sato, Ken Ichi ^a   Nishida, Eisuke ^b   Fukami, Yasuo ^a  

^a KOBE UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Anti peptide antibody; Autophosphorylation; Mitogen activated protein kinase; Reverse phosphate transfer; Tyrosine phosphorylation

Indexed keywords

ADENOSINE DIPHOSPHATE; MITOGEN ACTIVATED PROTEIN KINASE; MYELIN BASIC PROTEIN;

ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; IMMUNOPRECIPITATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; XENOPUS;

ANIMALS; ANTIBODIES; BINDING SITES; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; ENZYME ACTIVATION; KINETICS; MUTATION; PHOSPHORYLATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; TYROSINE; XENOPUS LAEVIS;

ANIMALIA; XENOPUS LAEVIS;

EID: 0029910659     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00322.x     Document Type: Article

References (35)

1. Andersen, N. G., Maller, J. L., Tonks, N. K. & Sturgill, T. W. (1990) Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase, Nature 343, 651-653.
2. Blenis, J. (1993) Signal transduction via the MAP kinases: proceed at your own RSK, Proc. Natl Acad. Sci. USA 90, 5889-5892.
3. Blumer, K. J. & Johnson, G. L. (1994) Diversity in function and regulation of MAP kinase pathways, Trends Biochem. Sci. 19, 236-240.
4. Cobb, M. H., Boulton, T. G. & Robbins, D. J. (1991) Extracellular signal-regulated kinases: ERKs in progress, Cell Regul. 2, 965-978.
5. Cobb, M. H. & Goldsmith, E. J. (1995) How MAPK Kinases are regulated, J. Biol. Chem. 270, 14843-14846.
6. Crews, C. M., Alessandrini, A. & Erikson, R. L. (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product, Science 258, 478-480.
7. Davis, R. J. (1993) The mitogen-activated protein kinase signal transduction pathway, J. Biol. Chem. 268, 14553-14556.
8. Foulkes, J. G., Chow, M., Gorka, C., Frackelton, A. R. Jr & Baltimore, D. (1985) Purification and characterization of a protein-tyrosine kinase encoded by the Abelson murine leukemia virus, J. Biol. Chem. 260, 8070-8077.
9. Fukami, Y. & Lipmann, F. (1983) Reversal of Rous sarcoma-specific immunoglobulin phosphorylation on tyrosine (ADP as phosphate acceptor) catalyzed by the sre gene kinase, Proc. Natl Acad. Sci. USA 80, 1872-1876.
10. Fukami, Y., Sato, K.-I., Ikeda, K., Kamisango, K., Koizumi, K. & Matsuno, T. (1993) Evidence for autoinhibitory regulation of the c-src gene product. A possible interaction between the src homology 2 domain and autophosphorylation site, J. Biol. Chem. 268, 1132-1140.
11. Gotoh, Y., Nishida, E., Matsuda, S., Shiina. N., Kosako, H., Shiokawa, K., Akiyama, T., Ohta, K. & Sakai, H. (1991a) In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase, Nature 349, 251-254.
12. Gotoh, Y., Moriyama, K., Matsuda, S., Okumura, E., Kishimoto, T., Kawasaki, H., Suzuki, K., Yahara, I., Sakai, H. & Nishida, E. (1991b) Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF, EMRO J. 10, 2661-2668.
13. Hubler, L., Gill, G. N. & Berties, P. J. (1989) Reversibility of the epidermal growth factor receptor self-phosphorylation reaction. Evidence for formation of a high energy phosphotyrosine bond, J. Biol. Chem. 264, 1558-1564.
14. Ikebe, M. & Hartshorne, D. J. (1986) Reverse reaction of smooth muscle myosin light chain kinase. Formation of ATP from phosphorylated light chain plus ADP, J. Biol. Chem. 261, 8249-8253.
15. Kong, S.-K., Chohan, I. S. & Wang, J. H. (1988) Autophosphorylation and autoactivation of spleen protein tyrosine kinase, J. Biol. Chem. 263, 14523-14530.
16. Kosako, H., Gotoh, Y., Matsuda, S., Ishikawa, M. & Nishida, E. (1992) Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation, EMBO J. 11, 2903-2908.
17. Kosako, H., Nishida, E. & Gotoh, Y. (1993) cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates, EMBO J. 12, 787-794.
18. Lacmmli, U. K. (1970) Cleavage of structural proreins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
19. Marshall, C. J. (1995) Specificity of receptor tyrosine kinase signalling: transient versus sustained extracellular signal-regulated kinase activation, Cell 80, 179-185.
20. Matsuda, S., Kosako, H., Takenaka, K., Moriyama, K., Sakai, H., Akiyama, T., Gotoh, Y. & Nishida, E. (1992) Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade, EMBO J. 11, 973-982.
21. Nishida, E. & Gotoh, Y. (1993) The MAP kinase cascade is essential for diverse signal transduction pathways, Trends Biochem. Sci. 18, 128-131.
22. Payne, D. M., Rossomando, A. J., Martino. P., Erickson, A. K., Her, J-H., Shabanowitz, J., Hunt. D. F., Weber, M. J. & Sturgill, T. W. (1991) Identification of the regulatory phosphorylation sites in pp42/ mitogen-activated protein kinase (MAP kinase), EMBO J. 10, 885-892.
23. Posada, J. & Cooper, J. A. (1992) Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes, Science 255, 212-215.
24. Robbins, D. J., Zhen, E., Owaki, H., Vanderbilt, C. A., Ebert, D., Geppert, T. D. & Cobb. M. H. (1993) Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro, J. Biol. Chem. 268, 5097-5106.
25. Rosen, O. M. & Erlichman, J. (1975) Reversible autophosphorylation of a cyclic 3′:5′-AMP-dependent protein kinase from bovine cardiac muscle, J. Biol. Chem. 250, 7788-7794.
26. mapk, Proc. Natl Acad. Sci. USA 89, 5779-5783.
27. Seger, R., Ahn, N. G., Boulton, T. G., Yancopoulos, G. D., Panayotatos, N., Radziejewska, E., Ericsson, L., Bratilen, R. L., Cobb, M. H. & Krebs, E. G. (1991) Microtubule-associated protein 2 kinases. ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation, Proc. Natl Acad Sci. USA 88, 6142-6146.
28. Seger, R., Ahn, N. G., Posada, J., Munar, E. S., Jensen, A. M., Cooper, J. A., Cobb, M. H. & Krebs, E. G. (1992) Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells, J. Biol. Chem. 267, 14373-14381.
29. Shizuta, Y., Beavo, J. A., Bechtel, P. J., Hofmann, F. & Krebs, E. G. (1975) Reversibility of adenosine 3′:5′-monophosphate-dependent protein kinase reactions, J. Biol. Chem. 250, 6891-6896.
30. Shizuta, Y, Khandelwal, R. L., Mailer, J. L., Vandenheede, J. R. & Krebs, E. G. (1977) Reversibility of phosphorylase kinase reaction, J. Biol. Chem. 252, 3408-3413.
31. Taylor, S. S. & Radzio-Andzelm, E. (1994) Three protein kinase structures define a common motif, Structure 2, 345-355.
32. Weber, W., Bertics, P. J. & Gill, G. N. (1984) Immunoaffinity purification of the epidermal growth factor receptor. Stoichiometry of binding and kinetics of self-phosphorylation, J. Biol. Chem. 259, 14631-14636.
33. Wu, J., Rossomando, A. J., Her, J.-H., Del Vecchio, R., Weber, M. J. & Sturgill, T. W. (1991) Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine, Proc. Natl Acad. Sci. USA 88, 9508-9512.
34. Zhang, E, Strand, A., Robbins, D., Cobb, M. H. & Goldsmith, E. J. (1994) Atomic structure of the MAP kinase ERK2 at 2.3 Å resolution. Nature 367, 704-711.
35. Zheng, C.-F. & Guan, K.-L. (1993) Dephosphorylation and inactivation of the mitogen-activated protein kinase by a mitogen-induced Thr/ Tyr protein phosphatase, J. Biol. Chem. 268, 16116-16119.