Molecular analysis of a β-lactam resistance gene encoded within the cephamycin gene cluster of Streptomyces clavuligerus

Journal of Bacteriology

Volumn 178, Issue 21, 1996, Pages 6266-6274

  Paradkar, Ashish S ^a   Aidoo, Kwamena A ^a,b   Wong, Annie ^a   Jensen, Susan E ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BETA LACTAM ANTIBIOTIC; CEPHALOSPORIN; CEPHAMYCIN; ISOPENICILLIN N SYNTHETASE; PENICILLIN BINDING PROTEIN; PENICILLIN G;

ANTIBIOTIC RESISTANCE; ANTIBODY SPECIFICITY; ARTICLE; ESCHERICHIA COLI; GENE CLUSTER; GENE LOCATION; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STREPTOMYCES CLAVULIGERUS;

ACTINOBACTERIA (CLASS); ESCHERICHIA COLI; STREPTOMYCES CLAVULIGERUS;

EID: 0029910623     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.21.6266-6274.1996     Document Type: Article

References (49)

1. Aharonowitz, Y., G. Cohen, and J. F. Martin. 1992. Penicillin and cephalosporin biosynthetic genes: structure, organization, regulation and evolution. Annu. Rev. Microbiol. 46:461-496.
2. Aharonowitz, Y., and A. L. Demain. 1978. Carbon catabolite regulation of cephamycin production in Streptomyces clavuligerus. Antimicrob. Agents Chemother. 14:159-164.
3. Aidoo, K. A., and S. E. Jensen. Unpublished data.
4. Aidoo, K. A., A. S. Paradkar, D. C. Alexander, and S. E. Jensen. 1993. Use of recombinant DNA techniques to study the production of β-lactam compounds in Streptomyces clavuligerus. p. 219-236. In V. P. Gullo, J. C. Hunter-Cevera, R. Cooper, and R. K. Johnson (ed.), Developments in industrial microbiology, vol. 33. Society for Industrial Microbiology. Fredericksburg, Va.
5. Aidoo, K. A., A. Wong, D. C. Alexander, R. A. Rittamer, and S. E. Jensen. 1994. Cloning, sequencing and disruption of a gene involved in clavulanic acid biosynthesis. Gene 147:41-46.
6. Altschul, S. F., G. Warren, W. Miller, E. W. Myers, and D. J. Lipmann. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
7. Asoh, S., H. Matsuzawa, F. Ishino, J. L. Strominger, M. Matsuhashi, and T. Ohta. 1986. Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12. Eur. J. Biochem. 160:231-238.
8. Bailey, C. R., and D. J. Winstanley. 1985. Inhibition of restriction in Streptomyces clavuligerus by heat treatment. J. Gen. Microbiol. 132:2945-2947.
9. Bibb, M. J., P. R. Findlay, and M. W. Johnson. 1984. The relationship between base composition and codon usage in bacterial genes and its use in the simple and reliable identification of protein-coding sequences. Gene 30:157-166.
10. Coque, J. J. R., P. Liras, and J. F. Martin. 1993. Genes for a β-lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans. EMBO J. 12:631-639.
11. Daniel, R. A., S. Drake, C. E. Buchanan, R. Scholle, and J. Errington. 1995. The Bacillus subtilis spoVD gene encodes a mother cell-specific penicillin-binding protein required for spore morphogenesis. J. Mol. Biol. 235:209-220.
12. Davies, J. 1994. Inactivation of antibiotics and dissemination of resistance genes. Science 264:375-382.
13. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
14. Doran, J. L., B. L. Leskiw, S. Aippersbach, and S. E. Jensen. 1990. Isolation and characterization of a β-lactamase inhibitory protein from Streptomyces clavuligerus and cloning and analysis of the corresponding gene. J. Bacteriol. 172:4909-4918.
15. El Kharroubi, A., P. Jacques, G. Piras, J. Van Beeumen, J. Coyette, and J. M. Ghuysen. 1991. The Enterococcus hirae R40 penicillin-binding protein 5 and the methicillin-resistant Staphylococcus aureus penicillin-binding protein 2′ are similar. Biochem. J. 280:463-469.
16. El Kharroubi, A., P. Jacques, G. Piras, J. Van Beeumen, J. Coyette, and J. M. Ghuysen. 1993. Modular design of the bi(multi?) functional penicillin-binding proteins, p. 319-333. In M. A. de Pedro, J. V. Holtje, and W. Loffelhardt (ed.), Bacterial growth and lysis: metabolism and structure of the bacterial sacculus. Plenum Publishing Corp., New York.
17. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Suton, W. FitzHugh, C. A. Fields, J. D. Gocayne, J. D. Scott, R. Shirley, L. I. Liu, A. Glodek, J. M. Kelly, J. F. Weidman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrman, N. S. M. Geoghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenza? Rd. Science 269:496-512.
18. Fontana, R., M. Aldegheri, M. Ligozzi, H. Lopez, A. Sucari, and G. Satta. 1994. Overproduction of a low-affinity penicillin-binding protein and high-level ampicillin resistance in Enterococcus faecium. Antimicrob. Agents Chemother. 38:1980-1983.
19. Fontana, R., P. Cerini, P. Longoni, A. Grossato, and P. Canepari. 1983. Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin. J. Bacteriol. 155:1343-1350.
20. Georgopapadakou, N. H. 1993. Penicillin-binding proteins and bacterial resistance to beta-lactams. Antimicrob. Agents Chemother. 37:2045-2053.
21. Ghuysen, J. M. 1991. Serine β-lactamases and penicillin-binding proteins. Annu. Rev. Microbiol. 45:37-67.
22. Gramajo, H. C., J. White, C. R. Hutchinson, and M. J. Bibb. 1991. Overproduction and localization of components of the polyketide synthase of Streptomyces glaucescens involved in the production of the antibiotic tetracenomycin C. J. Bacteriol. 173:6475-6483.
23. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Hartman, B. J., and A. Tomasz. 1984. Low-affinity penicillin-binding protein associated with β-lactam resistance in Staphylococcus aureus. J. Bacteriol. 158:513-516.
25. Hopwood, D. A., M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward, and H. Schrempf. 1985. Genetic manipulation of Streptomyces: a laboratory manual. The John Innes Foundation, Norwich, United Kingdom.
26. Horikawa, S., H. Nakazawa, and H. Ogawara. 1980. Penicillin-binding proteins in Streptomyces cacaoi and Streptomyces clavuligerus. J. Antibiot. 33: 1362-1368.
27. Jensen, S. E., D. C. Alexander, A. S. Paradkar, and K. A. Aidoo. 1993. Extending the β-lactam biosynthetic gene cluster in Streptomyces clavuligerus, p. 169-176. In R. H. Baltz, G. D. Hegeman, and P. L. Skatrud (ed.), Industrial microorganisms: basic and applied molecular genetics. American Society for Microbiology, Washington, D.C.
28. Jensen, S. E., and A. L. Demain. 1995. β-Lactams. p. 239-268. In L. C. Vining and C. Stuttard (ed.), Biochemistry and genetics of antibiotic biosynthesis. Butterworth Heinemann, Stoncham, Mass.
29. Kyte, J., and R. F. Dooliltle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
30. Leskiw, B. K., Y. Aharonowitz, M. Mevarech, S. Wolfe, L. C. Vining, D. W. S. Westlake, and S. E. Jensen. 1988. Cloning and nucleotide sequence determination of the isopenicillin N synthase gene from Streptomyces clavuligerus. Gene 62:187-196.
31. Martin, J. F., and P. Liras. 1989. Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites. Annu. Rev. Microbiol. 43:173-206.
32. Nakazawa, H., S. Horikawa, and H. Ogawara. 1981. Penicillin-binding proteins in Streptomyces strains. J. Antibiot. 34:1070-1072.
33. Ogawara, H. 1981. Antibiotic resistance in pathogenic and producing bacteria with special reference to β-lactam antibiotics. Microbiol. Rev. 45:591-619.
34. Ogawara, H., and S. Horikawa. 1980. Penicillin-binding proteins of Streptomyces cacaoi, Streptomyces olivaceus, and Streptomyces clavuligerus. Antimicrob. Agents Chemother. 17:1-7.
35. Paradkar, A. S., and S. E. Jensen. 1995. Functional analysis of the gene encoding the clavaminate synthase 2 isoenzyme involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. J. Bacteriol. 177:1307-1314.
36. Paradkar, A. S., R. H. Mosher, and S. E. Jensen. Comparative molecular genetics of β-lactam production. In W. R. Strohl (ed.), Biotechnology of industrial antibiotics. 2nd ed., in press. Marcel Dekker, Inc. New York.
37. Paradkar, A. S., A. K. Petrich, K. A. Aidoo, B. K. Leskiw, and S. E. Jensen. 1994. Transcriptional analysis and heterologous expression of a gene encoding the β-lactamase inhibitor protein from Streptomyces clavuligerus. Gene 144:31-36.
38. Paradkar, A. S., A. K. Petrich, and S. E. Jensen. Unpublished data.
39. Paradkar, A. S., A. Wong, and S. E. Jensen. Unpublished data.
40. Piras, G., A. El Kharroubi, J. Van Beeumen, E. Coeme, J. Coyette, and J. Ghuysen. 1990. Characterization of an Enterococcus hirae penicillin-binding protein 3 with low penicillin affinity. J. Bacteriol. 172:6856-6862.
41. Piras, G., D. Raze, A. E. Kharroubi, D. Hastir, S. Englobert, J. Coyette, and J. M. Ghuysen. 1993. Cloning and sequencing of the low-affinity penicillinbinding protein 3r-encoding gene of Enterococcus hirae S185: modular design and structural organization of the protein. J. Bacteriol. 175:2844-2852.
42. Reading, C., and M. Cole. 1977. Clavulanic acid: a beta-lactamase-inhibiting beta-lactam from Streptomyces clavuligerus. Antimicrob. Agents Chemother. 11:852-857.
43. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
44. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
45. Shiffman, D., M. Mevarech, S. E. Jensen, G. Cohen, and Y. Aharonowitz, 1988. Cloning and comparative sequence analysis of the gene coding for isopenicillin N synthase in Streptomyces. Mol. Gen. Genet. 214:562-569.
46. Song, M. D., M. Wachi, M. Doi, F. Ishino, and M. Matsuhashi. 1987. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett. 221:167-171.
47. Spratt, B. G. 1977. Properties of the penicillin-binding proteins of Escherichia coli K12. Eur. J. Biochem. 72:341-352.
48. Stuttard, C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. J. Gen. Microbiol. 128:115-121.
49. Ward, J. M., and J. E. Hodgson. 1993. The biosynthetic genes for clavulanic acid and cephamycin production occur as a 'super-cluster' in three Streptomyces. FEMS Microbiol. Lett. 110:239-242.