Separation of flavones by capillary electrophoresis: The influence of pKa on electrophoretic mobility

Phytochemical Analysis

Volumn 7, Issue 6, 1996, Pages 300-304

  Markham, Kenneth R ^a   McGhie, Tony K ^b  

^a CALLAGHAN INNOVATION   (New Zealand)

^b BSES LIMITED   (Australia)

Author keywords

Acidity; Borate complexation; Capillary electrophoresis; Electrophoretic mobility; Hydroxyflavonoids; pKa

Indexed keywords

ELECTROPHORETIC MOBILITY; FLAVONOIDS;

ACIDIC HYDROXYL GROUPS; BORATE COMPLEXATION; DIHYDROXYFLAVONE; FLAVONOID; HYDROXYFLAVONOID; LINEAR RELATIONSHIPS; LOWER AVERAGE; MOLECULAR SIZE; NOVEL SERIES; PKA;

CAPILLARY ELECTROPHORESIS;

BORIC ACID; FLAVONE DERIVATIVE; HYDROXYL GROUP;

ARTICLE; CAPILLARY ELECTROPHORESIS; COMPLEX FORMATION; ELECTROPHORETIC MOBILITY;

EID: 0029909929     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1099-1565(199611)7:6<300::aid-pca319>3.0.co;2-%23     Document Type: Article

References (18)

1. Jorgenson, J. W. and Lukacs, K. D. (1983). Capillary zone electrophoresis. Science 222, 266-272.
2. Lide, D. R. (ed.) (1994). Handbook of Chemistry and Physics, 74th Edn, Vol. 8, p. 46. CRC Press, Florida.
3. Mabry, T. J., Markham, K. R. and Thomas, M. B. (1970). The Systematic Identification of Flavonoids. pp. 50-51, Springer, New York.
4. Markham, K. R. (1982). Techniques of Flavonoid Identification. Academic Press, London.
5. McGhie, T. K. (1993). Analysis of sugarcane flavonoids by capillary zone electrophoresis (CZE). J. Chromatogr. 634, 707-712.
6. McGhie, T. K. and Markham, K. R. (1994). Separation of flavonols by capillary electrophoresis: The effect of structure on electrophoretic mobility. Phytochem. Anal. 5, 121-126.
7. Morin, P., Villard, F. and Dreux, M. (1993a). Borate complexation of flavonoid-O-glycosides in capillary electrophoresis. I. Separation of flavonoid-7-O-glycosides differing in their flavonoid aglycone. J. Chromatogr. 628, 153-160.
8. Morin, P., Villard, F., Dreux, M. and Andre, P. (1993b). Borate complexation of flavonoid-O-glycosides in capillary electrophoresis. II. Separation of flavonoid-3-O-glycosides differing in their sugar moiety. J. Chromatogr. 628, 161-169.
9. Pietta, P. G., Mauri, P. L., Rava, A. and Sabbatini, G. (1991). Application of micellar electrokinetic capillary chromatography to the determination of flavonoid drugs. J. Chromatogr. 549, 367-373.
10. Pietta, P. G., Mauri, P. L., Bruno, A. and Gardana, C. (1994). Influence of structure on the behaviour of flavonoids in capillary electrophoresis. Electrophoresis 15, 1326-1331.
11. Porter, L. J. and Markham, K. R. (1970). The aluminium(III) complexes of hydroxyflavones in absolute methanol. Part I. Ligands containing only one chelating site. J. Chem. Soc., C 344-349.
12. Seitz, U., Bonn, G., Oefner, P. and Popp, M. (1991). Isotachophoretic analysis of flavonoids and phenolcarboxylic acids of relevance to phytopharmaceutical industry. J. Chromatogr. 559, 499-504.
13. Seitz, U., Oefner, P. G., Nathakarnkitool, S., Popp, M. and Bonn, G. K. (1992). Capillary electrophoretic analysis of flavonoids. Electrophoresis 13, 35-38.
14. Tomas-Barberan, F. A. (1995). Capillary electrophoresis: a new technique in the analysis of plant secondary metabolites. Phytochem. Anal. 6, 177-192.
15. Tyukavkina, N. A. and Pogodaeva, N. N. (1975). Ultraviolet absorption of flavonoids VIII. Ionization constants of kaempferol and quercetin. Khim. Prir. Soedin. (English Edn.) 6, 741-743.
16. Vandee Casteele, K., Geiger, H. and Van Sumere, C. F. (1982). Separation of flavonoids by reverse-phase high performance liquid chromatography. J. Chromatogr. 240, 81-94.
17. Wolfbeis, O. S., Leiner, M., Hochmuth, P. and Geiger, H. (1984). Absorption and fluorescence spectra, pKa values, and fluorescence lifetimes of monohydroxyflavones and monomethoxyflavones. Ber. Bunsenges. Phys. Chem. 88, 759-767.
18. Wolfbeis, O. S., Begum, M. and Geiger, H. (1987). The fluorescence properties of luteolins. Monatshefte Chemie 118, 1403-411.