GGAAAT motifs play a major role in transcriptional activity of the human insulin gene in a pancreatic islet beta-cell line MIN6

Diabetologia

Volumn 39, Issue 12, 1996, Pages 1462-1468

  Tomonari, A ^a   Yoshimoto, K ^a   Tanaka, M ^a   Iwahana, H ^a   Miyazaki, J ^b   Itakura, M ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

GG motif; insulin gene; MIN6; pancreatic islet; transcription

Indexed keywords

INSULIN; OLIGONUCLEOTIDE;

ANIMAL CELL; ARTICLE; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL;

3T3 CELLS; ANIMALS; BASE SEQUENCE; CELL LINE; DNA PROBES; GENE DELETION; HUMAN GROWTH HORMONE; HUMANS; INSULIN; ISLETS OF LANGERHANS; MICE; MUTATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); TRANS-ACTIVATION (GENETICS); TRANSFECTION;

EID: 0029909871     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001250050599     Document Type: Article

References (22)

1. Boam DSW, Clark AR, Docherty K (1990) Positive and negative regulation of the insulin gene by multiple transacting factors. J Biol Chem 265: 8285-8296
2. Whelan J, Poon D, Weil PA, Stein R (1989) Pancreatic β-cell-type-specific expression of the rat insulin II gene is controlled by positive and negative cellular transcriptional elements. Mol Cell Biol 9: 3253-3259
3. Karlsson O, Edlund T, Moss JB, Rutter WJ, Walker MD (1987) A mutational analysis of the insulin gene transcription control region: Expression in beta cells is dependent on two related sequences within the enhancer. Proc Natl Acad Sci USA 84: 8819-8823
4. German MS, Moss LG, Wang J, Rutter WJ (1992) The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical β-cell nuclear complexes. Mol Cell Biol 12: 1777-1788
5. German M, Ashcroft S, Docherty K et al. (1995) The insulin gene promoter. A simplified nomenclature. Diabetes 44: 1002-1004
6. Ohlsson H, Karlsson K, Edlund T (1993) IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J 12: 4251-4259
7. MacFarlane WM, Read ML, Gilligan M, Bujalska I, Docherty K (1994) Glucose modulates the binding activity of the β-cell transcription factor IUF1 in a phosphorylation-dependent manner. Biochem J 303: 625-631
8. Petersen HV, Serup P, Leonard J, Michelsen BK, Madsen OD (1994) Transcriptional regulation of the human insulin gene is dependent on the homeodomain protein STF1/ IPF1 acting through the CT boxes. Proc Natl Acad Sci USA 91: 10465-10469
9. Nelson C, Shen L, Meister A, Fodor E, Rutter WJ (1990) Pan: a transcriptional regulator that binds chymotrypsin, insulin, and AP-4 enhancer motifs. Gene Dev 4: 1035-1043
10. Melloul D, Ben-Neriah Y, Cerasi E (1993) Glucose modulates the binding of an islet-specific factor to a conserved sequence within the rat I and the human insulin promoters. Proc Natl Acad Sci USA 90: 3865-3869
11. German MS, Wang J (1994) The insulin gene contains multiple transcriptional elements that respond to glucose. Mol Cell Biol 14: 4067-4075
12. Sharma A, Stein R (1994) Glucose-induced transcription of the insulin gene is mediated by factors required for β-cell-type-specific expression. Mol Cell Biol 14: 871-879
13. Miyazaki J-I, Araki K, Yamato E et al. (1990) Establishment of a pancreatic β cell line that retains glucose-inducible insulin secretion: Special reference to expression of glucose transporter isoforms. Endocrinology 127: 126-132
14. Ishihara H, Asano T, Tsukuda K et al. (1993) Pancreatic beta-cell line MIN6 exhibits characteristics of glucose metabolism and glucose-stimulated insulin secretion similar to those of normal islets. Diabetologia 36: 1139-1149
15. Bell GI, Pictet R, Rutter WJ, Cordell B, Tischer E, Goodman HM (1980) Sequence of the human insulin gene. Nature 284: 26-32
16. Seiden RF, Howie KB, Rowe ME, Goodman HM, Moore DD (1986) Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Mol Cell Biol 6: 3173-3179
17. Luckow B, Schütz G (1987) CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res 15: 5490
18. Berger J, Hauber J, Hauber R, Geiger R, Cullen BR (1988) Secreted placental alkaline phosphatase: A powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66: 1-10
19. Bronstein I, Fortin JJ, Voyta JC et al. (1994) Chemiluminescent reporter gene assays: sensitive detection of the GUS and SEAP gene products. BioTechniques 17: 172-177
20. Andrew NC, Faller DV (1991) A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res 19: 2499
21. Clark AR, Wilson ME, Leibiger I, Scott V, Docherty K (1995) A silencer and an adjacent positive element interact to modulate the activity of the human insulin promoter. Eur J Biochem 232: 627-632
22. Shieh S-Y, Tsai M-J (1991) Cell-specific and ubiquitous factors are responsible for the enhancer activity of the rat insulin II gene. J Biol Chem 266: 16708-16714