Diagnosis of Mediterranean spotted fever by cultivation of Rickettsia conorii from blood and skin samples using the centrifugation-shell vial technique and by detection of R. conorii in circulating endothelial cells: A 6-year follow-up

Journal of Clinical Microbiology

Volumn 34, Issue 11, 1996, Pages 2722-2727

  La Scola, Bernard ^a   Raoult, Didier ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD CULTURE; BOUTONNEUSE FEVER; CENTRIFUGATION; DIAGNOSTIC ACCURACY; ENDOTHELIUM CELL; GOOD LABORATORY PRACTICE; HUMAN; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; RICKETTSIA CONORII; SKIN CULTURE;

BACTERIA (MICROORGANISMS); RICKETTSIA; RICKETTSIA CONORII;

EID: 0029908711     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.11.2722-2727.1996     Document Type: Article

References (28)

1. Dierksheide, W. C. 1987. Medical decisions: interpreting clinical tests. ASM News 53:677-680.
2. Donowitz, G., and G. Mandell. 1995. Acute pneumonia, p. 619-637. In G. Mandell, J. Bennett, and R. Dolin, (ed.), Principles and practice of infectious diseases. Churchill Livingstone, Ltd., Edinburgh.
3. Drancourt, M., F. Georges, P. Brouqui, J. Sampol, and D. Raoult. 1992. Diagnosis of Mediterranean spotted fever by indirect immunofluorescence of Rickettsia conorii in circulating endothelial cells isolated with monoclonal antibody-coated immmunomagnetic beads. J. Infect. Dis. 166:660-663.
4. Eremeeva, M., X. Yu, and D. Raoult. 1994. Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA. J. Clin. Microbiol. 32:803-810.
5. Espejo-Arenas, E., and D. Raoult. 1989. First isolates of Rickettsia conorii in Spain using a centrifugation-shell vial assay. J. Infect. Dis. 159:1158-1159. (Letter.)
6. Furuya, Y., Y. Yoshida, T. Katayama, F. Kawamori, S. Yamamoto, N. Ohashi, A. Tamura, and A. Kawamura. 1991. Specific amplification of Rickettsia tsutsugamushi DNA from clinical specimens by polymerase chain reaction. J. Clin. Microbiol. 29:2628-2630.
7. Greene, W., D. Walker, and B. Cain. 1978. Fatal viscerotropic Rocky Mountain spotted fever. A report of case diagnosed by immunofluorescence. Am. J. Med. 64:523-528.
8. Kelly, P. J., L. Beati, P. Mason, R. Makombe, L. Matthewman, D. Raoult, and M. Dreary. 1992. African tick-bite fever - a new spotted fever group rickettsiosis under an old name. Lancet 340:982-983.
9. Kelly, P. J., L. Beati, L. A. Matthewman, P. R. Mason, G. A. Dasch, and D. Raoult. 1994. A new pathogenic spotted fever group rickettsia from Africa. Am. J. Trop. Med. Hyg. 97:129-137.
10. Lambert, M., T. Dugernier, G. Bigaignon, J. Rahier, and P. Piot. 1984. Mediterranean spotted fever in Belgium. Lancet ii:1038. (Letter.)
11. Marrero, M., and D. Raoult. 1989. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am. J. Trop. Med. Hyg. 40:197-199.
12. Musso, D., and D. Raoult. 1995. Coxiella burnetii blood cultures from acute and chronic Q-fever patients. J. Clin. Microbiol. 33:3129-3132.
13. Raoult, D., C. De Micco, H. Gallais, and M. Toga. 1984. Laboratory diagnosis of Mediterranean spotted fever by immunofluorescent demonstration of Rickettsia conorii in cutaneous lesions. J. Infect. Dis. 150:145-148.
14. Raoult, D., and M. Drancourt. 1991. Antimicrobial therapy of rickettsial diseases. Antimicrob. Agents Chemother. 35:2457-2462.
15. Raoult, D., P. Roussellier, G. Vestris, and J. Tamalet. 1987. In vitro antibiotic susceptibility of Rickettsia rickettsii and Rickettsia conorii: plaque assay and microplaque colorimetric assay. J. Infect. Dis. 155:1059-1062.
16. Raoult, D., H. Tissot-Dupont, P. Caraco, P. Brouqui, M. Drancourt, and C. Charrel. 1992. Mediterranean spotted fever in Marseille: descriptive epidemiology and the influence of climatic factors. Eur. J. Epidemiol. 8:192-197.
17. Raoult, D., P. Zuchelli, P. J. Weiller, C. Charrel, J. L. San Marco, H. Gallais, and P. Casanova. 1986. Incidence, clinical observations and risk factors in the severe form of Mediterranean spotted fever among patients admitted to hospital in Marseilles 1983-1984. J. Infect. 12:111-116.
18. Regnery, R. L., C. L. Spruill, and B. D. Plikaytis. 1991. Genomic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J. Bacteriol. 173:1576-1589.
19. Silverman, D. J. 1984. Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro. Infect. Immun. 44:545-553.
20. Silverman, D. J., and S. B. Bond. 1984. Infection of human vascular endothelial cells by Rickettsia rickettsii. J. Infect. Dis. 149:201-206.
21. Teysseire, N., and D. Raoult. 1992. Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever. J. Clin. Microbiol. 30:455-460.
22. Tringali, G., C. Occhino, S. Mansueto, D. H. Walker, and D. Raoult. 1986. Fièvre boutonneuse méditerranéenne diagnostiquée pendant la saison froide: quatre cas. Med. Mal. Infect. 3:183-184.
23. Tzianabos, T., B. E. Anderson, and J. E. McDade. 1989. Detection of Rickettsia rickettsii DNA in clinical specimens by using polymerase chain reaction technology. J. Clin. Microbiol. 27:2866-2868.
24. Walker, D. H. 1990. The role of host factors in the severity of spotted fever and typhus rickettsioses. Ann. N. Y. Acad. Sci. 590:10-19.
25. Walker, D. H., B. G. Cain, and P. M. Olmstead. 1978. Laboratory diagnosis of Rocky Mountain spotted fever by immunofluoresccnt demonstration of rickettsia in cutaneous lesions. Am. J. Clin. Pathol. 69:619-623.
26. Walker, D. H., B. G. Cain, and P. M. Olmstead. 1978. Specific diagnosis of Rocky Mountain spotted fever by immunofluoresccnce demonstration of Ricketlsia rickettsii in biopsy of skin. Public Health Lab. 36:96-100.
27. Werner, A., C. Cobbs, and D. Kaye. 1967. Studies of the bacteremia of bacterial endocarditis. JAMA 202:199.
28. Woodward, T. E., C. E. Pedersen, Jr., C. N. Oster, L. R. Bagley, J. Romberger, and M. J. Snyder. 1976. Prompt confirmation of Rocky Mountain spotted fever: identification of rickettsiae in skin tissues. J. Infect. Dis. 134: 297-301.