Mapping of the entomocidal fragment of Spodoptera-specific Bacillus thuringiensis toxin CryIC

Molecular and General Genetics

Volumn 253, Issue 1-2, 1996, Pages 11-19

  Strizhov, N ^a   Keller, M ^b   Konez Kalman Z ^a   Regev, A ^b   Sneh, B ^b   Schell, J ^a   Koncz, C ^a   Zilberstein, A ^b  

^a MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

^b TEL AVIV UNIVERSITY   (Israel)

Author keywords

endotoxin; CryIC; Proteolytic processing; Site specific mutagenesis; Spodoptera littoralis

Indexed keywords

BACILLUS THURINGIENSIS TOXIN; CORE PROTEIN; INSECTICIDE; MUTANT PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; TRYPSIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS THURINGIENSIS; CARBOXY TERMINAL SEQUENCE; CONSENSUS SEQUENCE; ESCHERICHIA COLI; GENE MAPPING; LEPIDOPTERA; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; STRUCTURE ACTIVITY RELATION; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLONING, MOLECULAR; CONSENSUS SEQUENCE; ENDOPEPTIDASES; ENDOTOXINS; GENES, BACTERIAL; HEMOLYSIN PROTEINS; INSECTICIDES; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SPODOPTERA; TRYPSIN;

BACILLUS THURINGIENSIS; ESCHERICHIA COLI; HEXAPODA; INSECTA; LEPIDOPTERA; SPODOPTERA; SPODOPTERA LITTORALIS;

EID: 0029908130     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050290     Document Type: Article

References (46)

1. Adang M, Staver M, Rocheleau T, Leighton J, Barker R, Thompson D (1985) Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis suhsp. kurstaki HD-73 and their toxicity to Manduca sexta. Gene 36:289-300
2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
3. Aronson AI (1993) The two faces of Bacillus thuringiensis: insecticidal proteins and post-exponential survival. Mol Microbiol 7:489-496
4. Aronson AI, Wu D, Zhang C (1995) Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene. J Bacteriol 177:4059-4065
5. Bietlot H, Carey PR, Kaplan H, Lessard T, Pozsgay M (1989) Facile preparation and characterization of the toxin from Bacillus thuringiensis var. kurstaki. Biochem J 260:87-91
6. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
7. Chen XJ, Lee MK, Dean DH (1993) Site-directed mutations in a highly conserved region of Bacillus thuringisis δ-endotoxin affect inhibition of short circuit current across Bombyx mori midguts. Proc Natl Acad Sci USA 90:9041-9045
8. Choma C, Surewicz W, Carey P, Pozgay M, Raynor T, Kaplan H (1990) Unusual proteolysis of the protoxin and toxin from Bacillus thuringiensis. Structural implications. Eur J Biochem 189:523-527
9. Convents D, Cherlet M, Van Damme J, Lasters I, Lauwereys M (1991) Two structural domains as a general fold of the toxic fragment of the Bacillus thuringiensis δ-endotoxins. Fur J Biochem 195:631-635
10. Deveraux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387-395
11. Dhaese P, De Greve H, Decramer H, Schell J, Van Montagu M (1979) Rapid mapping of transposon insertion and deletion mutations in the large Ti plasmids of Agrobacterium tumefaciens. Nucleic Acids Res 7: 1837-1849
12. Gazit E, Bach D, Kerr ID, Sansom MSP, Chejanovsky N, Shai Y (1994) The α-5 segment of Bacillus thuringiensis δ-endotoxin: in vitro activity, ion channel formation and molecular modelling. Biochem J. 304:895-902.
13. Grochulski P, Masson L, Borisova S, Pusztai-Carey M, Schwartz J-L, Brousseau R, Cygler M (1995) Bacillus thuringiensis CryA(a) insecticidal toxin: crystal structure and channel formation. J Mol Biol 254:447-464
14. Haider MZ, Ellar DJ (1989) Functional mapping of an entomocidal δ-endotoxin. J Mol Biol 208: 183-194
15. Hamal M, Brownbridge M, Broza M, Sneh B (1991) Screening for highly effective isolates of Bacillus thuringiensis against Spodoptera exempta and Spodoptera littoralis . Phytoparasitica 19:9-21
16. Höfte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53:242-255
17. Höfte H. Greve H de, Seurinck J, Jansens S, Mahillon J, Ampe C, Vandekerckhove J, Vanderbruggen H, Van Montagu M, Zabeau M, Vaeck M (1986) Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. Eur J Biochem 161:273-280
18. Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11 : 291-298
19. Honée G, Salm T van der, Visser B (1988) Nucleotide sequence of crystal protein gene isolated from B. thuringiensis subspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species. Nucleic Acids Res 16:6240
20. Knight PJK, Knowles BH, Ellar DJ (1995) Molecular cloning of an insect aminopeptidase N that serves as a receptor for Bacillus thuringiensis CryIA(C) toxin. J Biol Chem 270: 17765-17770
21. Knowles BH (1994) Mechanism of action of Bacillus thuringiensis insecticidal δ-endotoxins. Adv Insect Physiol 24:275-308
22. Kronstad JW, Whiteley HR (1986) Three classes of homologous Bacillus thuringensis crystal-protein genes. Gene 43:29-40
23. Laenimli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
24. Li J, Carrol J, Ellar DJ (1991) Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5Å resolution. Nature 353:815-821
25. Lu H, Rajamohan F, Dean DH (1994) Identification of amino acid residues of Bacillus thuringiensis δ-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. J Bacteriol 176:5554-5559
26. Martens JWM, Visser B, Vlak JM, Bosch D (1995) Mapping and characterization of the entomocidal domain of the Bacillus thuringiensis CryIA(b) protoxin. Mol Gen Genet 247:482-487
27. Masson L, Lu Y, Mazza A, Brousseau R, Adang MJ (1995) The CryIA(C) receptor purified from Manduca sexta displays multiple specificities. J Biol Chem 270:20309-20315
28. Nakamura K, Murai-Nishioka R, Shimizu M, Oshie K, Mikitani K, Oeda K, Ohkawa H (1992) Insecticidal activity and processing in larval gut juices of genetically engineered 130-kDa proteins of Bacillus thuringiensis subsp. aizawai. Biosci Biotech Biochem 56:1-7
29. Nakamura K, Oshie K, Shimizu M, Takada Y.,Oeda K, Ohkawa H (1990) Construction of chimeric insecticidal proteins between the 130-kDa and 135-kDa proteins of Bacillus thuringiensis subsp. aizawai for analysis of structure-function relationship. Agric Biol Chem 54:715-724
30. Nishimoto T, Yoshisue H, Ihara K, Sakai H, Komano T (1994) Functional analysis of block 5, one of the highly conserved amino acid sequences in the 130-kDa CryIVA protein produced by Bacillus thuringiensis subsp. israeliensis. FEBS Lett 348: 249-254
31. Perlak FJ, Deaton RW, Armstrong TA, Fuchs RL, Sims SR, Green-plate JT, Fischhoff DA (1990) Insect resistant cotton plants. Biotechnology 8:939-943
32. Rajamohan F, Alcantara E, Lee MK, Chen XJ, Curtiss A, Dean DH (1995) Single amino acid changes in domain II of Bacillus thuringiensis CryIAb δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles. J Bacteriol 177: 2276-2282
33. Salm T van der, Bosch D, Honée G, Feng L, Musterman E, Bakker P, Stiekema WJ. Visser B (1994) Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy. Plant Mol Biol 26:51-59
34. Sambrook J, Fritsch EF, Maniatis T (1991) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
35. Sanchis V, Lereclus D, Menou G, Chaufaux J, Lecadet MM (1988) Multiplicity of δ-endotoxin genes with different specificities in Bacillus thuringiensis aizawai 7.29. Mol Microbiol 2:393-404
36. Sanchis V, Lereclus D, Menou G, Chaufaux J, Guo S, Lecadet MM (1989) Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active δ-endotoxin of Bacillus thuringiensis aizawai 7.29. Mol Microbiol 3:229-238
37. Schmidt J, John M, Wieneke U, Krüssmann, Schell J (1986) Expression of the modulation gene nodA in Rhizobium meliloti and localization of the gene product in the cytosol. Proc Natl Acad Sci USA 83:9581-9585
38. Schnepf EH (1995) Bacillus thuringiensis toxins: regulation, activities and structural diversity. Curr Opin Biotechnol 6:305-312
39. Smith GP, Ellar DJ (1994) Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC δ-endotoxin affects insecticidal specificity. Biochem J 302:611-616
40. Smith GP, Ellar DJ, Keeler SJ, Scip CE (1994) Nucleotide sequence and analysis of an insertion sequence from Bacillus thuringiensis related to IS150. Plasmid 32:10-18
41. Studier W, Rosenberg A, Dunn J, Dubendorff J (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185:60-89
42. Vadlamudi RK, Weber E, Ji I, Bulla LA (1995) Cloning and expression of a receptor for an insecticidal toxin of Bacillus thurigiensis. J Biol Chem 270:5490-5494
43. Visser B, Munsterman E, Stoker A, Dirkse WG (1990) A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific crystal protein. J Bacteriol 172:6783-6788
44. Wabiko H, Yasuda E (1995) Bacillus thuringiensis protoxin: location of toxic border and requirement of non-toxic domain for high-level in vivo production of active toxin. Microbiology 141: 629-639
45. Wabiko H, Raymond KC, Bulla LA (1986) Bacillus thuringiensis entomocidal protoxin gene sequence and gene product analysis. DNA 5:305-314
46. Walters FS, Slatin SL, Kuleszka CA, English LH (1993) Ion channel activity of N-terminal fragments from CryIA(c) delta-endotoxin. Biochem Biophys Res Commun 196:921-926