PCR phenotyping of cytokines, growth factors and their receptors and bone matrix proteins in human osteoblast-like cell lines

Bone

Volumn 19, Issue 5 SUPPL., 1996, Pages 437-445

  Bilbe, G ^a   Roberts, E ^b   Birch, M ^b   Evans, D B ^a  

^a CIBA GEIGY LTD   (Switzerland)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Bone matrix; Cytokine; Growth factor; Osteoblast; PCR; Receptors

Indexed keywords

ALPHA INTERFERON; CYTOKINE; CYTOKINE RECEPTOR; ENDOTHELIAL CELL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; FIBROBLAST GROWTH FACTOR; GROWTH FACTOR; GROWTH FACTOR RECEPTOR; INTERLEUKIN 4 RECEPTOR; INTERLEUKIN 7; LYMPHOTOXIN; MATRIX PROTEIN; MESSENGER RNA; ONCOSTATIN M;

ARTICLE; BONE MATRIX; CELL LINE; CELL LINEAGE; CELL MATURATION; CONTROLLED STUDY; EXPLANT; GENE TARGETING; HUMAN; HUMAN CELL; OSTEOBLAST; OSTEOSARCOMA; PHENOTYPE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRABECULAR BONE;

BASE SEQUENCE; BONE MORPHOGENETIC PROTEINS; BONE NEOPLASMS; CYTOKINES; ELECTROPHORESIS, AGAR GEL; GENE EXPRESSION REGULATION; GROWTH SUBSTANCES; HUMANS; MOLECULAR SEQUENCE DATA; OSTEOBLASTS; OSTEOSARCOMA; PHENOTYPE; POLYMERASE CHAIN REACTION; RECEPTORS, GROWTH FACTOR; TUMOR CELLS, CULTURED;

EID: 0029907737     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(96)00254-2     Document Type: Article

References (90)

1. Allan, E. H., Hilton, D. J., Brown, M. A., Evely, R. S., Yumita, S., Metcalf, D., Gough, N. M., Ng, K. W., Nicola, N. A., and Martin, T. J. Osteoblasts display receptors for and responses to leukemia inhibitory factor. J Cell Physiol 145:110-119; 1990.
2. 3, PTH and glucocorticoids. Metab Bone Dis Rel Res 5:229-234; 1984.
3. Beresford, J. N., Taylor, G. T., and Triffitt, J. T. Interferons and bone. A comparison of the effects of interferon-alpha and interferon-gamma in cultures of human bone-derived cells and an osteosarcoma cell line. Eur J Biochem 193:589-597; 1990.
4. Birch, M., Ginty, A., Walsh, C., Fraser, W., Gallagher, J., and Bilbe, G. PCR detection of cytokines in normal human and pagetic osteoblast-like cells. J Bone Miner Res 8:1155-1162; 1993.
5. 2+ in murine osteoclasts. J Biol Chem 269:13817-13824; 1994.
6. 5a. Clover, J. and Gowen, M. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblast phenotype? Bone 15:585-591; 1994.
7. Chomczynski, P. and Sacchi, N. Single step method of RNA isolation by acid guanidinium thiocyanate phenol-chloroform extraction. Anal Biochem 162:156-159; 1987.
8. Chaudhary, L. R., Spelsberg, T. C., and Riggs, B. L. Production of various cytokines by normal human osteoblast-like cells in response to interleukin-1 beta and tumour necrosis factor-alpha: Lack of regulation by 17 beta-estradiol. Endocrinology 130:2528-2534; 1992.
9. Chaudhary, L. R. and Avioli, L. V. Dexamethasone regulates IL-1 beta and TNF-alpha-induced interleukin 8 production in human bone marrow stromal and osteoblast-like cells. Calcif Tissue Int 55:16-20; 1994.
10. Chenu, C., Valentin-Opran, A., Chavassieux, P., Saez, S., Menuier, P. J., and Delmas, P. D. Insulin-like growth factor-I hormonal regulation by growth hormone and by 1,25(OH)2D3 and activity on human osteoblast-like cells in short-term cultures. Bone 11:81-86; 1990.
11. Claesson-Welsh, L. Platelet-derived growth factor receptor signals. J Biol Chem 269:32023-32026; 1994.
12. Cornish, J., Calon, K., King, A., and Reid, I. R. The effect of leukemia inhibitory factor on bone in vivo. Endocrinology 132:1359-1366; 1993.
13. Demulder, A., Suggs, S. V., Zsebo, K. M, Scarcez, T., and Roodman, G. D. Effects of stem cell factor on osteoclast-like cell formation in long-term human marrow cultures. J Bone Miner Res 7:1337-1344; 1992.
14. Elias, J. A., Tang, W., and Horowitz, M. C. Cytokine and hormonal stimulation of human osteosarcoma interleukin-11 production. Endocrinology 136:489-498; 1995.
15. Evans, D. B., Bunning, R. A., and Russell, R. G. G. The effects of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on human osteoblast-like cells. Biochem Biophys Res Commun 160:588-595; 1989.
16. Evans, D. B., Smith, A. G., Rathjen, P. D., Williams, M. M., Heath, J. K., and Gowen, M. Regulation of leukemia inhibitory factor (LIF) production in human bone cells in vitro. In: Christiansen, C. and Overgaard, K., Eds, Osteoporosis. Denmark: Osteopress ApS; 1990, pp. 324-326.
17. 2 in the inhibitory regulation of osteocalcin production by human osteoblast-like cells in response to cytokines and systemic hormones. Biochem Biophys Res Commun 167:194-202; 1990.
18. 2, plasminogen activator, osteocalcin and alkaline phosphatase by osteoblast-like cells derived from human bone. Biochem Biophys Res Commun 166:208-216; 1990.
19. 2 microglobulin on human bone-derived cells in vitro. Biochem Biophys Res Commun 175:795-803; 1991.
20. Evans, D. B., Hipskind, R. A., Baccarini, M., and Bilbe, G. Multiple bone active agents induce c-fos via pathways targetting MAP kinase in human osteosarcoma cells. J Bone Miner Res 8:1030A; 1993.
21. Evans, D. B., Gerber, B., and Feyen, J. H. M. Recombinant human leukemia inhibitory factor is mitogenic for human bone-derived osteoblast-like cells. Biochem Biophys Res Commun 199:220-226; 1994.
22. Felix, R., Fleisch, H., and Elford, P. R. Bone resorbing cytokines enhance release of macrophage colony-stimulating activity by the osteoblastic cell MC3T3-E1. Calcif Tissue Int 44:356-360; 1989.
23. Finkelman, R. D., Mohan, S., Linkhart, T. A., Abraham, S. M., Boussy, J. P., and Baylink, D. J. PTH stimulates the proliferation of TE-85 human osteosarcoma cells by a mechanism not involving either increased cAMP or increased secretion of IGF-1, IGF-II, or TGF beta. Bone Miner 16:89-100; 1992.
24. Fitzsimmons, R. J., Ryaby, J. T., Magee, F. P., and Baylink, D. J. IGF-II receptor number is increased in TE-85 osteosarcoma cell by combined magnetic fields. J Bone Miner Res 10:812-819; 1995.
25. 3 stimulates the synthesis of matrix γ-carboxyglutamic acid protein by osteosarcoma cells. J Biol Chem 263:911-916; 1988.
26. Fuller, K., Owens, J. M., and Chambers, T. J. Macrophage inflammatory protein-1 alpha and IL-8 stimulate the motility but suppress the resorption of isolated rat osteoclasts. J Immunol 154:6065-6072; 1995.
27. Gimble, J. M., Wanker, F., Wang, C. S., Bass, H., Wu, X., Kelly, K., Yancopoulos, G. D., and Hill, M. R. Regulation of bone marrow stromal cell differentiation by cytokines whose receptors share the gp130 protein. J Cell Biochem 54:122-133; 1994.
28. Girasole, G., Passen, G., Jilka, R. L., and Manolagalas, S. C. Interleukin 11: A new cytokine critical for osteoclast development. J Clin Invest 93:1516-1524; 1994.
29. Globus, R. K., Plouet, J., and Gospodarowicz, D. Cultured bovine bone cells synthesize basic fibroblast growth factor and store it in their extracellular matrix. Endocrinology 124:1539-1547; 1989.
30. Goad, D. L. and Tashjian, A. H., Jr. Insulin-like growth factor-I inhibits parathyroid hormone-stimulated and enhances prostaglandin E2-stimulated adenosine3′, 5′-monophosphate production by human osteoblast-like SaOS-2 cells. Endocrinology 133:1585-1592; 1993.
31. Gowen, M., Chapman, K., Littlewood, A., Hughes, D., Evans, D., and Russell, G. Production of tumour necrosis factor by human osteoblasts is modulated by other cytokines but not by osteotropic hormones. Endocrinology 126:1250-1255; 1990.
32. Gowen, M. and Russell, R. G. G. Cytokines and Bone Metabolism. Boca Raton CRC; 1991.
33. Greenfield, E. M., Gornik, S. A., Horowitz, M. C., Donahue, H. J., and Shaw, S. M. Regulation of cytokine expression in osteoblasts by parathyroid hormone: rapid stimulation of interleukin-6 and leukemia inhibitory factor. J Bone Miner Res 8:1163-1171; 1993.
34. Hamblin, A. S. Cytokines and cytokine receptors. Oxford: Oxford University Press; 1993.
35. Heath, J. K. Growth Factors. Oxford: Oxford University Press; 1993.
36. Hill, P. A., Reynolds, J. J., and Meikle, M. C. Osteoblasts mediate insulin-like growth factor-I and -II stimulation of osteoclast formation and function. Endocrinology 136:124-131; 1995.
37. Hughes, F. J. and Howells, G. L. Interleukin 11 inhibits bone formation. Calc Tissue Int 53:362-364; 1993.
38. Hughes, F. J. and Howells, G. L. Interleukin 6 inhibits bone formation in vitro. Bone Miner 21:21-28; 1993.
39. Horowitz, M. C., Coleman, D. L., Ryaby, J. T., and Einhorn, T. A. Osteotropic agents induce the differential secretion of granulocyte-macrophage colony-stimulating factor by the osteoblast cell line MC3T3-E1. J Bone Miner Res 4:911-921; 1989.
40. Horowitz, M. C., Coleman, D. L., Flood, P. M., Kupper, T. S., and Jilka, R. L. Parathyroid hormone and lipopolysaccharide induce murine osteoblast-like cells to secrete a cytokine indistinguishable from granulocyte-macrophage colony-stimulating factor. J Clin Invest 83:149-157; 1989.
41. Ishibashi, H., Karube, S., Yamakawa, A., and Koshihara, Y. Interleukin-4 stimulates pro-alpha 1 (VI) collagen gene expression in cultured human osteoblast-like cells. Biochem Biophys Res Commun 211:727-734; 1995.
42. Jay, P., Lorenzo, J., Centrella, M., Bruce, A., and Horowitz, M. Oncostatin M: A new cytokine with osteoblast activating activity. J Bone Miner Res 8:S302; 1993.
43. Kasono, K., Sato, K., Stao, Y., Tsushima, T., Shizume, K., and Demura, H. Inhibitory effect of interleukin-4 on osteoclast-like cell formation in mouse bone marrow culture. Bone Miner 21:179-188; 1993.
44. Kassem, M., Blum, W., Ristelli, J., Mosekilde, L., and Eriksen, E. F. Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro. Calc Tissue Int 52:222-226; 1993.
45. Lacey, D. L., Erdmann, J. M., Tan, H. L., and Ohara, J. Murine osteoblast interleukin 4 receptor expression: upregulation by 1,25 dihydroxyvitamin D3. J Cell Biochem 53:122-134; 1993.
46. Lacey, D. L., Erdmann, J. M., Shima, M., Kling, S., Matayoshi, A., Ohara, J., and Perkins, S. L. Interleukin-4 enhances osteoblast macrophage colony stimulating factor, but not interleukin 6 production. Calcif Tissue Int 55:21-28; 1994.
47. Lacey, D. L., Erdmann, J. M., Teitelbaum, S. L., Tan, H. L., Ohara, J., and Shioi, A. Interleukin-4, interferon gamma, and prostaglandin E impact the osteoclastic cell forming potential of murine bone marrow macrophages. Endocrinology 136:2367-2376; 1995.
48. Lee, M. Y., Lottsfeldt, J. L., and Fevold, K. L. Identification and characterization of osteoclast progenitors by clonal analysis of hematopoietic cells. Blood 80:1710-1716; 1992.
49. Lewis, D., Liggitt, H., Effmann, E., Motley, S., Teitelbaum, S., Jepsen, K., Goldstein, S., Bonadio, J., Carpenter, J., and Perlmutter, R. Osteoporosis induced in mice by over production of IL-4. Proc Natl Acad Sci USA 90:11618-11622; 1992.
50. Li, Y. P. and Stashenko, P. Proinflammatory cytokines tumour necrosis factor alpha and IL-6, but not IL-1, down-regulate the osteocalcin gene promoter. J Immunol 148:788-794; 1992.
51. Lind, M., Deleuran, B., Yssel, H., Fink-Eriksen, E., and Thestrup-Pedersen, K. IL-4 and IL-13, but not IL-10, are chemotactic factors for human osteoblasts. Cytokine 7:78-82; 1995.
52. Littlewood, A. J., Aarden, L. A., Evans, D. B., Russell, R. G. G., and Gowen, M. Human osteoblast-like cells do not respond lo interleukin-6. J Bone Miner Res 6:141-148; 1991.
53. Littlewood, A. J., Russell, J., Harvey, G. R., Hughes, D. E., Russell, R. G., and Gowen, M. The modulation of the expression of IL-6 and its receptor in human osteoblasts in vitro. Endocrinology 129:1513-1520; 1991.
54. Malaval, L., Gupta, A. K., and Aubin, J. E. Leukemia inhibitory factor inhibits osteogenic differentiation in rat calvarial cell cultures. Endocrinology 136:1411-1419; 1995.
55. Merry, K. and Gowen, M. The transcriptional control of TGF-β in human osteoblast-like cells is distinct from that of IL-1β. Cytokine 4:171-179; 1992.
56. Middleton, J., Arnott, N., Walsh, S., and Beresford, J. Osteoblasts and osteoclasts in adult human osteophyte tissue express the mRNAs for insulin-like growth factors I and II and the type I IGF receptor. Bone 16:287-293; 1995.
57. Mohan, S., Bautista, C. M., Herring, S. J., Linkhart, T. A., and Baylink, D. J. Development of valid methods to measure insulin-like growth factor-I and -II in bone cell conditioned medium. Endocrinology 126:2534-2542; 1990.
58. Mohan, S., Strong, D. D., Hilliker, S., Malpe, R., Lee, K., Farley, J., and Baylink, D. J. Dibutyryl cyclic adenosine monophosphate differentially regulates cell proliferation in low and high alkaline phosphatase SaOS-2 human osteosarcoma cells: evidence for mediation by the insulin-like growth factor-II system. J Cell Physiol 156:462-468; 1993.
59. Mundy, G. R. Ciba Foundation Symposium 157: Clinical Applications of TGF-βp 1991, New York: Wiley; 1991.
60. Mundy, G. R. Cytokines and growth factors in the regulation of bone remodelling. J Bone Miner Res 8(Suppl. 2): S505-S510; 1993.
61. Neben, S. and Turner, K. The biology of interleukin 11. Stem Cells 11:156-162; 1993.
62. Nakano, Y., Watanabe, K., Morimoto, I., Okada, Y., Ura, K., Sato, K., Kasono, K., Nakamura, T., and Eto, S. Interleukin-4 inhibits spontaneous and parathyroid hormone-related protein-stimulated osteoclast formation in mice. J Bone Miner Res 10:1533-1539; 1994.
63. Nohtomi, K., Sato, K., Shizume, K., Yamazaki, K., Demura, H., Hosoda, K., Murata, Y., and Seo, H. Stimulation of interleukin-4 of cell proliferation and mRNA expression alkaline phosphatase and collagen type I in human osteoblast-like cells of trabecular bone. Bone Miner 27:69-79; 1994.
64. Panagakos, F. S. and Kumar, S. Modulation of proteases and their inhibitors in immortal human osteoblast-like cells by tumor necrosis factor alpha in vitro. Inflammation 18:243-265; 1994.
65. Ralston, S. H., Hoey, S. A., Gallacher, S. J., Adamson, B. B., and Boyle, I. T. Cytokine and growth factor expression in Paget's disease: Analysis by reversetranscription/polymerase chain reaction. Br J Rheumatol 33:620-625; 1994.
66. Reid, L. R., Lowe, C., Cornish, J., Skinner, S. J., Hilton, D. J., Willson, T. A., Gearing, D. P., and Martin, T. J. Leukemia inhibitory factor: A novel bone-active cytokine. Endocrinology 126:1416-1420; 1990.
67. Riancho, J. A., Zarrabeitia, M. T., Mundy, G. R., Yoneda, T., and Gonzalez-Macias, J. Effects of interleukin-4 on the formation of macrophages and osteoclast-like cells. J Bone Miner Res 11:1337-1344; 1993.
68. Riancho, J. A., Zarrabeitia, M. T., Olmos, J. M., Amado, J. A., and Gonzalez-Macias, J. Effects of interleukin-4 on human osteoblast-like cells. Bone Miner 21:53-61; 1993.
69. Riancho, J. A., Gonzalez-Marcias, J., Amado, J. A., Olmas, J. M., and Fernandez-Luna, J. L. Interleukin-4 as a bone regulatory factor: effects on murine osteoblast-like cells. J Endocrinol Invest 18:174-179; 1995.
70. Riancho, J. A., Zarrabeitia, M. T., Fernandez-Luna, J. L., and Gonzalez-Maricas, J. Mechanisms controlling nitric oxide synthesis in osteoblasts. Mol Cell Endocrinol 107:87-92; 1995.
71. Rifas, L., Kenney, J. S., Marcelli, M., Pacifici, R., Cheng, S-L., Dawson, L. L., and Aviloi, L. V. Production of interleukin-6 in human osteoblasts and human bone marrow stromal cells: Evidence that induction by interleukin-1 and tumor necrosis factor-α is not regulated by ovarian steroids. Endocrinology 136:4056-4067; 1995.
72. Rodan, G. A. and Noda, M. Gene expression in osteoblastic cells. Crit Rev Gene Exp 1:85-98; 1991.
73. Roodman, G. D. Role of cytokines in the regulation of bone resorption. Calc Tissue Int 53:894-98; 1993.
74. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Press; 1989.
75. Sato, N. and Mirayama, A. Multimeric cytokine receptors: common versus specific functions. Curr Opin Cell Biol 6:174-179; 1994.
76. Shioi, A., Teitelbaum, S. L., Ross, F. P., Welgus, H. G., Suzuki, H., Ohara, J., and Lacey, D. L. Interleukin-4 inhibits murine osteoclast formation in vitro. J Cell Biochem 47:272-277; 1991.
77. Strom, P., Ljunghall, S., and Melhus, H. Adult human osteoblast-like cells do not express insulin-like growth factor-I. Biochem Biophys Res Commun 199:78-82; 1994.
78. Taichman, R. S. and Emerson, S. G. Human osteoblasts support hematopoiesis through the production of granulocyte colony-stimulating factor. J Exp Med 179:1677-1682; 1994.
79. Takahashi, S., Reddy, S. V., Dallas, M., Devlin, R., Chou, J. Y., and Roodman, G. D. Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation. Endocrinology 136:1441-1449; 1995.
80. Takaishi, T., Matsui, T., Tsukamoto, T., Ito, M., Taniguchi, T., Fukase, M., and Chihara, K. TGF-beta-induced macrophage colony-stimulating factor gene expression in various mesenchymal cell lines. Am J Physiol 267:C25-31; 1994.
81. Tamura, T., Udagawa, N., Takahashi, N., Miyaura, C, Tanaka, S., Yamada, Y., Koishihara, Y., Ohsugi, Y., Kumaki, K., Taga, T., Kishimoto, T., and Suda, T. Soluble interleukin-6 receptor triggers osteoclast formation by interleukin 6. Proc Natl Acad Sci USA 90:11924-11928; 1993.
82. Ueno, K., Katayama, T., Miyamoto, T., and Koshihara, Y. Interleukin-4 enhances in vitro mineralization in human osteoblast-like cells. Biochem Biophys Res Commun 189:1521-1526; 1992.
83. Van Beek, E., van der Wee-Pals, L., van de Ruit, M., Nijweide, P., Papapoulos, S., and Lowik, C. Leukemia inhibitory factor inhibits osteoclastic resorption, growth, mineralization and alkaline phosphatase activity in fetal mouse metacarpal bones in culture. J Bone Miner Res 8:191-198; 1993.
84. Vandenabeele, P., Declercq, W., Beyaert, R., and Fiers, W. Two tumour necrosis factor receptors: Structure and function. Trends Cell Biol 5:392-399; 1995.
85. van Hille, B., Richener, H., Evans, D. B., Green, J. R., and Bilbe, G. Identification of two subunit A isoforms of the vacuolar H(+)-ATPase in human osteoclastoma. J Biol Chem 268:7075-7080; 1993.
86. Van Vlasselaer, P. Leukemia inhibitory factor (LIF): a growth factor with pleiotropic effects on bone biology. Prog Growth Factor Res 4:337-353; 1992.
87. Walsh, C, Birch, M. A., Fraser, W., Lawton, R., Dorgan, J., Walsh, S., Sansom, D., Beresford, J. N., and Gallagher, J. A. Expression and secretion of parathyroid hormone-related protein by human bone-derived cells in vitro: Effects of glucocorticoids. J Bone Miner Res 10:17-25; 1995.
88. Yang, Y. C. Interleukin 11: An overview. Stem Cells Dayt 11:474-486; 1993.
89. Yajima, M. The effects of various cell growth factors and cyclosporin A, an immunosuppressive agent, on cloned osteoblastic cell line, MC3T3-E1 cells. Nippon Naibunpi Gakkai Zasshi 64:1187-1198; 1988.
90. Zwickl, M., Zaninetta, D., McMaster, G. K., and Hardman, N. Selective cloning of B cell hybridoma-specific rearranged immunoglobulin gene loci using the polymerase chain reaction. J Immunol Methods 130:49-55; 1990.