Double fluorescent flow cytometric assessment of bacterial internalization and binding by epithelial cells

Cytometry

Volumn 25, Issue 4, 1996, Pages 381-387

  De Boer, Elizabeth C ^a   Bevers, Rob F M ^a   Kurth, Karl Heinz ^a   Schamhart, Denis H J ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Epithelial cells; Escherichia coli; Flow cytometry; Immuofluorescence; Methodology; Mycobacterium bovis BCG; Phagocytosis

Indexed keywords

BCG VACCINE;

ARTICLE; BLADDER CARCINOMA; BLADDER EPITHELIUM; CANCER IMMUNOTHERAPY; CELL LINE; CONTROLLED STUDY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; MYCOBACTERIUM BOVIS; PHAGOCYTOSIS; PRIORITY JOURNAL; TECHNIQUE;

COLORING AGENTS; EPITHELIAL CELLS; EPITHELIUM; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; MYCOBACTERIUM BOVIS; PHAGOCYTOSIS; PHYCOERYTHRIN; TUMOR CELLS, CULTURED; URINARY BLADDER;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM BOVIS BCG;

EID: 0029907710     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19961201)25:4<381::AID-CYTO10>3.3.CO;2-M     Document Type: Article

References (15)

1. Becich MJ, Carroll S, Ratliff TL: Internalization of bacillus Calmette-Guerin by bladder tumor cells. J Urol 145:1316-1324, 1991.
2. Catanzaro A, Wright SD: Binding of Mycobacterium avium-Mycobacterium intracellulare to human leukocytes. Infect Immun 58: 2951-2956, 1990.
3. Drevets DA, Campbell PA: Macrophage phagocytosis: Use of fluorescence microscopy to distinguish between extracellular and intracellular bacteria. J Immunol Methods 142:31-38, 1991.
4. Dunn PA, Eaton WR, Lopatin ED, McEntire JE, Papermaster BW: Lymphokine stimulated macrophage phagocytosis of fluorescent microspheres: A rapid new assay. J Immunol Methods 64:71-83, 1983.
5. Fattorossi A, Nisini R, Pizzolo JG, D'Amelio R: New, simple flow cytometry technique to discriminate between internalized and membrane-bound particles in phagocytosis. Cytometry 10:320-325, 1989.
6. Giaimis J, Lombard Y, Poindron P, Muller CD: Flow cytometry distinction between adherent and phagocytized yeast particles. Cytometry 17:173-178, 1994.
7. Hed J: The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Microbiol Lett 1:357-361, 1977.
8. Hed J, Hallden G, Johansson SGO, Larsson P: The use of fluorescence quenching in flow cytometry to measure the attachment and ingestion phases in phagocytosis in peripheral blood without prior cell separation. J Immunol Methods 101:119-125, 1987.
9. Ichinose M, Hara N, Sawada M, Maeno T: A flow cytometric assay reveals an enhancement of phagocytosis by platelet activating factor in murine peritoneal macrophages. Cell Immunol 156:508-518, 1994.
10. Isberg RR: Discrimination between intracellular uptake and surface adhesion of bacterial pathogens. Science 252:934-938, 1991.
11. Kuroda K, Brown EJ, Telle WB, Russell DG, Ratliff TL: Characterization of the internalization of bacillus Calmette Gucrin by human bladder tumor cells. J Clin Invest 91:69-76, 1993.
12. Lamm DL, Crawford ED, Blumenstein B, Crissman J, De Vere White R, Wolf M, Lowe B, Sarosdy M, Schellhammer P, Sagalowsky A, Smith J, Grossman HB, Flanigan R, Moon T, Brendler C, Coltman C: SWOG 8795: A randomized comparison of bacillus Calmette-Guérin and mitomycin C prophylaxis in stage Ta and T1 transitional cell carcinoma of the bladder. J Urol 149:282A, 1993.
13. Schneider B, Thanhauser A, Jocham D, Loppnow H, Vollmer E, Galle J, Had HD, Ulmer AJ, Böhle A: Specific binding of bacillus Calmette-Guerin to urothelial tumor cells in vitro. World J Urol 12:337-344, 1994.
14. Schöning R, Verstijnen CPHJ, Kuijper S, Kolk AHJ: Enzyme immunoassay for identification of heat killed mycobacteria belonging to the Mycobacterium tuberculosis and Mycobacterium avium complexes and derived from early cultures. J Clin Microbiol 28:708-713, 1990.
15. Teppema JS, De Boer EC, Steerenberg PA, Van Der Meijden APM: Morphological aspects of the interaction of bacillus Calmette-Guerin (BCG) with urothelial cells in vivo and in vitro: Relevance for antitumor activity? Urol Res 20:219-228, 1992.