Comparative study of classical, colorimetric and immunologic staining methods for the assessment of tumor cell viability

Tumor Biology

Volumn 17, Issue 6, 1996, Pages 354-361

  Basha, G ^a   Yap, P ^a   Penninckx, F ^a  

^a UNIVERSITY HOSPITALS LEUVEN   (Belgium)

Author keywords

Apoptosis; Cell viability; Cytotoxicity; Dye exclusion assays; Tetrazolium salts; Trypan blue; Tumoricidal activity

Indexed keywords

CHLORHEXIDINE CETRIMIDE; POVIDONE IODINE; TETRAZOLIUM; TOSYLCHLORAMIDE SODIUM; TRYPAN BLUE; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CELL VIABILITY; COLORIMETRY; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; IMMUNOASSAY; PRIORITY JOURNAL; TUMOR CELL;

EID: 0029907186     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1159/000218000     Document Type: Article

References (26)

1. Alpini G, Phillips JO, Vroman B, La Russo NF: Recent advances in the isolation of liver cells, Flepatology 1994;20:494-514.
2. Niderau C, Fronhoffs K, Klonowski H, Schulz HU: Active pancreatic digestive enzymes show striking differences in their potential to damage isolated rat pancreatic acinar cells, J Lab Clin Med 1995;125:265-275.
3. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing, Cancer Res 1987;47:936-942.
4. Denizot F, Lang R: Rapid colorimetric assay for cell growth and survival, Modifications to the tetrazol-ium dye procedure giving improved sensitivity and reliability, J Immunol Methods 1986;89:271-277.
5. Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, J Immunol Methods 1983;65:55-63.
6. Sladowski D, Steer SJ, Clothier RH, Balls M: An improved MTT assay, J Immunol Methods 1993; 157:203-207.
7. Wilson JK, Sargent JM, Elgie AW, Hill JG, Taylor CG: A feasibility study of the MTT assay for chemosensitivity testing in ovarian malignancy, Br J Cancer 1990;62:189-194.
8. Gavrieli Y, Sherman Y, Ben-Sason SHA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation, J Cell Biol 1992;119:493-501.
9. Lennon SV, Martin SJ, Cotter TG: Dose-dependent induction of apop-tosis in human tumour cell lines by widely diverging stimuli, Cell 1991;24:203-214.
10. Fogh J, Fogh JM, Orfeo TH: One hundred and twenty-seven cultured human tumour cell lines producing tumours in nude mice, J Natl Cancer Inst 1977;59:221-226.
11. Heerd BG, Houston MA, Augen-licht LH: Potentiation by specific short-chain fatty acids of differentiation and apoptosis in human colonic carcinoma cell line, Cancer Res 1994;54:3288-3294.
12. Scheppach W, Bartam HP, Richter F: Role of short chain fatty acids in the prevention of colorectal cancer, Eur J Cancer 1995;31:1077-1080.
13. Hague A, Elder DJE, Hicks DJ, Pa-raskeva C: Apoptosis in colorectal tumour cells: Induction by the short-chain fatty acids, butyrate, pro-prionate and acetate and by the bile salt deoxycholate, Int J Cancer 1995;60:400-406.
14. Umpleby HC, Williamson RCN: The efficacy of agents employed to prevent anastomotic recurrence in colorectal carcinoma, Ann R Coll Surg Engl 1984;66:192-194.
15. Arends MJ, Morris RG, Wyllie AH: Apoptosis, The role of endonuclease, Am J Pathol 1990; 136:593-608.
16. Li Y, Chopp M, Jiang N, Yao F, Zaloga C: Temporal profile of in situ DNA fragmentation after transient middle cerebral artery occlusion in rat, J Cereb Blood Flow Metab 1995;15:389-397.
17. Freshney RI: Measurement of Cytotoxicity and Viability, Culture of Animal Cells: A Manual of Basic Technique, New York, Wiley-Liss Inc., 1994. chapt 19. pp 287-307.
18. Gaffney DK, Schober SL, Sieber F: Merocyanine 540-sensitized photoinactivation of leukemia cells: Role of oxygen and effects on plasma membrane integrity and mitochondrial respiration, Exp Hematol 1990;18:23-26.
19. Me Ewen BF, Telford JN, Handel-man CT, Arion WJ: A critical evaluation of the use of filipin-permeabil-ized rat hepatocytes to study functions of the endoplasmic reticulum in situ, Cell Biochem Funct 1987;5: 263-272.
20. Marks DC, Belov L, Davey MV, Davey RA, Kidman AD: The MTT cell viability assay for cytotoxicity testing in multidrug-resistant human leukemic cells, Leuk Res 1992; 16:1165-1173.
21. Kerr JFR, Wyllie AH, Currie AR: Apoptosis: A basic biological phenomenon with wide ranging implications in tissue kinetics, Br J Cancer 1972;26:239-257.
22. Wyllie AH: Apoptosis (the 1992 Frank Rose Memorial Lecture), Cancer 1993;67:205-208.
23. Williams GT: Programmed cell death: Apoptosis and oncogenesis, Cell 1991;65:1097-1098.
24. Sen S, D'lncalci M: Apoptosis, biochemical events and relevance to cancer chemotherapy, FEBS Lett 1992;307:122-127.
25. Gerschenson LE, Rotello RJ: Apoptosis: A different type of cell death, FASEBJ 1992;6:2450-2455.
26. Van Der Loosdrecht AA, Ossenko-pele GJ, Beelen RJH, Broekhoven MG, Drager AM, Langenhuijsen MMAC: Apoptosis in tumor necrosis factor-a-dependent, monocyte-mediated leukemic cell death: A functional, morphologic and flow-cytometric anaylsis, Exp Hematol 1993;21:1628-1639.