Structure prediction of the EcoRV DNA methyltransferase based on mutant profiling, secondary structure analysis, comparison with known structures of methyltransferases and isolation of catalytically inactive single mutants

Protein Engineering

Volumn 9, Issue 5, 1996, Pages 413-423

  Jeltsch, Albert ^a   Sobotta, Tatjana ^a   Pingoud, Alfred ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

In vitro evolution; Neutral mutations; Protein structure prediction; Random mutagenesis; Restriction modification system

Indexed keywords

DNA METHYLTRANSFERASE; MUTANT PROTEIN; RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN SECONDARY STRUCTURE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN STRUCTURE, SECONDARY; RESTRICTION MAPPING; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC);

EID: 0029904146     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.5.413     Document Type: Review

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