Saporin, a ribosome-inactivating protein used to prepare immunotoxins, induces cell death via apoptosis

British Journal of Haematology

Volumn 93, Issue 4, 1996, Pages 789-794

  Bergamaschi, Gaetano ^a   Perfetti, Vittorio ^b   Tonon, Laura ^a   Novella, Annunziata ^a   Lucotti, Claudia ^a   Danova, Marco ^a   Glennie, Martin J ^c   Merlini, Giampaolo ^b   Cazzola, Mario ^a,b  

^a UNIVERSITY OF PAVIA   (Italy)

^b FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

^c SOUTHAMPTON GENERAL HOSPITAL   (United Kingdom)

Author keywords

apoptosis; immunotoxin; lymphocyte; monoclonal antibodies; saporin

Indexed keywords

BISPECIFIC ANTIBODY; CD22 ANTIGEN; IMMUNOTOXIN; SAPORIN;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; LEUKEMIA CELL; LYMPHOCYTE; NEUTROPHIL; PRIORITY JOURNAL; RIBOSOME;

EID: 0029903272     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1996.d01-1730.x     Document Type: Article

References (27)

1. Barbieri, L. & Stirpe, F. (1982) Ribosome-inactivating proteins from plants: properties and possible causes. Cancer Surveys, 1, 489-520.
2. Benedetti, G., Bondesan, P., Caracciolo, D., Cherasco, C., Ruggieri, D., Gastaldi, M.E., Pileri, A., Gianni, A.M. & Tarella, C. (1994) Selection and characterisation of early hematopoietic progenitors using an anti-CD71/SO6 immunotoxin. Experimental Hematology, 22, 166-173.
3. Bergamaschi, G., Cazzola, M., Dezza, L. Savino, E., Consonni, L. & Lappi, D. (1988) Killing of K562 cells with conjugates between human transferrin and a ribosome-inactivating protein (SO-6). British Journal of Haematology, 68, 379-384.
4. Bergamaschi, G., Danova, M., Ponchio, L., Rosti, V. & Cazzola, M. (1993) Inhibitors of tyrosine phosphorylation induce apoptosis in human leukemic cell lines. Leukemia, 7, 2012-2018.
5. Bergamaschi, G., Rosti, V., Danova, M., Lucotti, C. & Cazzola, M. (1994) Apoptosis: biological and clinical aspects. Haematologica, 79, 86-93.
6. Bonardi, M.A., Bell, A., French, R.R., Gromo, G., Hamblin, T., Modena, D., Tutt, A.L. & Glennie, M.J. (1992) Initial experience in treating human lymphoma with a combination of bispecific antibody and saporin. International Journal of Cancer, 7, 73-77.
7. Cazzola, M., Bergamaschi, G., Dezza, L., D'Uva, R., Ponchio, L. & Rosti, V. (1991) Cytotoxic activity of an anti-transferrin receptor immunotoxin on normal and leukemic human hematopoietic progenitors. Cancer Research, 51, 536-541.
8. Collins, R.J., Harmon, B.V., Souvlis, T., Pope, J.H. & Kerr, J.F.R. (1991) Effects of cycloheximide on B-chronic lymphocytic leukaemic and normal lymphocytes in vitro: induction of apoptosis. British Journal of Cancer, 64, 518-522.
9. Flavell, D.J., Boehm, D.A., Okayama, K., Kohler, J.A. & Flavell, S.U. (1994) Therapy of human T-cell acute lymphoblastic leukemia in severe combined immunodeficient mice with two different anti-CD7-saporin immunotoxins containing hindered or non-hindered disulphide cross-linkers. International Journal of Cancer, 58, 407-414.
10. French, R.R., Courtenay, A.E., Ingamells, S., Stevenson, G.T. & Glennie, M.J. (1991) Cooperative mixtures of bispecific F(ab′)2 antibodies for delivering saporin to lymphoma in vitro and in vivo, Cancer Research, 51, 2353-2361.
11. French, R.R., Hamblin, T.J., Bell, A.J., Tutt, A.L. & Glennie, M.J. (1995) Treatment of B-cell lymphomas with combination of bispecific antibodies and saporin. Lancet, 346, 223-224.
12. Gasperi-Campani, A., Zauli, G., Roncuzzi, L., Valvassori, L., Vitale, L., Gaggioli, L. & Bagnara, G.P. (1989) Differential activity of saporin 6 on normal and leukemic hemopoietic cells. Experimental Hematology, 17, 755-759.
13. 2 antibody for the delivery of saporin in the treatment of lymphoma. Journal of Immunology, 141, 3662-3670.
14. 2 antibody containing thioeter-linked Fab′γ fragments. Journal of Immunology, 139, 2367-2375.
15. Haslett, C., Guthrie, L.A., Kopaniak, M.M., Johnston, R.B. & Henson, P.M. (1985) Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide. American Journal of Pathology. 119, 101-110.
16. Hertler, A.A. & Frankel, A.E. (1989) Immunotoxins: a clinical review of their use in the treatment of malignancies. Journal of Clinical Oncology, 7, 1932-1942.
17. Lappi, D.A., Esch, F.S., Barbieri, L., Stirpe, F. & Soria, M. (1985) Characterization of a Saponaria officinalis seed ribosome-inactivating protein: immunoreactivity and sequence homologies. Biochemical and Biophysical Research Communications, 129, 934-942.
18. Mason, D.Y., Stein, H., Gerdes, J., Pulford, K.A.F., Ralfkiaer, E., Falini, B., Erber, W.N., Micklem, K. & Gatter, K.C. (1987) Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells. Blood, 69, 836-840.
19. Morland, B.J., Barley, J., Boehm, D., Flavell, S.U., Ghaler, N., Kohler, J.A., Okayama, K., Wilkins, B. & Flavell, D.J. (1994) Effectiveness of HB2(anti-CD-7)-saporin immunotoxin in an in vivo model of human T-cell leukaemia developed in SCID mice. British Journal of Cancer, 69, 279-285.
20. Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, F. & Riccardi, C. (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Journal of Immunological Methods, 139, 271-279.
21. Preijers, F.W.M.B. (1993) Rationale for the clinical use of immunotoxins: monoclonal antibodies conjugated to ribosome-inactivating proteins. Leukemia and Lymphoma, 9, 293-304.
22. Savill, J.S., Wyllie, A.H., Henson, I.E., Walport, M.J., Henson, P.M. & Haslett, C. (1989) Macrophage phagocytosis of aging neutrophils in inflammation. Journal of Clinical Investigation, 83, 865-875.
23. Stirpe, F., Gasperi-Campani, A., Barbieri, L., Falasca, A., Abbondanza, A. & Stevens, W.A. (1983) Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort). of Agrostemma githago L. (corn cockle) and of Asparagus officinalis (asparagus), and from the latex of Hura crepitans L. (sandbox tree). Biochemical Journal, 216, 617-625.
24. Tazzari, P.L., Bolognesi, A., de Totero, D., Lemoli, R.M., Fortuna, A., Conte, R., Crumpton, M.J. & Stirpe, F. (1994) Immunotoxins containing saporin linked to different CD2 monoclonal antibodies: in vitro evaluation. British Journal of Haematology, 86, 97-105.
25. Thiele, D.L., Kurosaka, M. & Lipsky, P.E. (1983) Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotropic agent, L-leucine methyl ester. Journal of Immunology, 131, 2282-2290.
26. Vitetta, E.S., Fulton, R.J., May, R.D., Till, M. & Uhr, J.W. (1987) Redesigning nature's poisons to create anti-tumor reagents. Science, 238, 1098-1104.
27. Williams, G.T., Smith, C.A., Spooncer, E., Dexter, T.M. & Taylor, D.R. (1990) Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis. Nature, 343, 76-79.