The polymerase-like core of brome mosaic virus 2a protein, lacking a region interacting with viral 1a protein in vitro, maintains activity and 1a selectivity in RNA replication

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4729-4736

  Smirnyagina, Ekaterina ^a   Lin, N A Sheng ^a,c   Ahlquist, Paul ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Bock Laboratories   (United States)

^c INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; VIRUS RNA;

ARTICLE; GENE MAPPING; MOSAIC VIRUS; PLANT VIRUS; PRIORITY JOURNAL; PROTEIN BINDING; RNA SYNTHESIS; VIRUS MORPHOLOGY; VIRUS REPLICATION;

EID: 0029903012     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4729-4736.1996     Document Type: Article

References (50)

1. Ahlquist, P. 1992. Bromovirus RNA replication and transcription. Curr. Opin. Genet. Dev. 2:71-76.
2. Ahlquist, P., V. Luckow, and P. Kaesberg. 1981. Complete nucleotide sequence of brome mosaic virus RNA3. J. Mol. Biol. 153:23-38.
3. Ahlquist, P., E. G. Strauss, C. M. Rice, J. H. Strauss, J. Haseloff, and D. Zimmern. 1985. Sindbis virus proteins nsP1 and nsP2 contain homology to nonstructural proteins from several RNA plant viruses. J. Virol. 53:536-542.
4. Allison, R. F., M. Janda, and P. Ahlquist. 1988. Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus. J. Virol. 62:3581-3588.
5. Allison, R. F., M. Janda, and P. Ahlquist. 1989. Sequence of cowpea chlorotic mottle virus RNA2 and 3 and evidence of a recombination event during bromovirus evolution. Virology 172:321-330.
6. Allison, R. F., C. Thompson, and P. Ahlquist. 1990. Regeneration of a functional RNA virus genome by recombination between deletion mutants and requirements for cowpea chlorotic mottle virus 3a and coat genes for systemic infection. Proc. Natl. Acad. Sci. USA 87:1820-1824.
7. Cornelissen, B. J. C., and J. F. Bol. 1984. Homology between the proteins encoded by tobacco mosaic virus and two tricornaviruses. Plant Mol. Biol. 3:379-384.
8. Dawson, W. O., and K. M. Lento. 1990. Regulation of tobamovirus gene expression. Adv. Virus Res. 38:307-342.
9. De Jong, W., and P. Ahlquist. 1992. A hybrid plant RNA virus made by transferring the noncapsid movement protein from a rod-shaped to an icosahedral virus is competent for systemic infection. Proc. Natl. Acad. Sci. USA 89:6808-6812.
10. De Jong, W., and P. Ahlquist. 1995. Host-specific alterations in viral RNA accumulation and infection spread in a brome mosaic virus isolate with an expanded host range. J. Virol. 69:1485-1492.
11. De Jong, W., K. Mise, and P. Ahlquist. 1994. The multigenic nature of RNA virus adaptation to plants. Trends Microbiol. 2:29-31.
12. Dinant, S., M. Janda, P. A. Kroner, and P. Ahlquist. 1993. Bromovirus RNA replication and transcription require compatibility between the polymerase-and helicase-like viral RNA synthesis proteins. J. Virol. 67:7181-7189.
13. French, R., M. Janda, and P. Ahlquist. 1986. Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyledonous plant cells. Science 231:1294-1297.
14. Goelet, P., G. P. Lomonosoff, P. J. G. Butler, M. E. Akam, M. J. Gait, and J. Karn. 1982. Nucleotide sequence of tobacco mosaic virus RNA. Proc. Natl. Acad. Sci. USA 79:5818-5822.
15. Goldbach, R., O. Le Gall, and J. Wellink. 1991. Alpha-like viruses in plants. Semin. Virol. 2:19-25.
16. Gorbalenya, A. E., and E. V. Koonin. 1989. Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acids Res. 17:8413-8440.
17. Haseloff, J., P. Goelet, D. Zimmern, P. Ahlquist, R. Dasgupta, and P. Kaesberg. 1984. Striking similarities in amino acid sequence among nonstructural proteins encoded by RNA viruses that have dissimilar genomic organization. Proc. Natl. Acad. Sci. USA 81:4358-4362.
18. Huisman, M. J., H. J. M. Linthorst, J. F. Bol, and B. J. C. Cornelissen. 1988. The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. J. Gen. Virol. 69:1789-1798.
19. Ishikawa, M., T. Meshi, F. Motoyoshi, N. Takamatsu, and Y. Okada. 1986. In vitro mutagenesis of the putative replicase genes of tobacco mosaic virus. Nucleic Acids Res. 14:8291-8305.
20. Ishikawa, M., T. Meshi, T. Ohno, and Y. Okada. 1991. Specific cessation of minus-strand RNA accumulation at an early stage of tobacco mosaic virus infection. J. Virol. 65:861-868.
21. Janda, M., and P. Ahlquist. 1993. RNA-dependent replication, transcription, and persistence of hrome mosaic virus RNA replicons in S. cerevisiae. Cell 72:961-970.
22. Janda, M., R. French, and P. Ahlquist. 1987. High efficiency T7 polymerase synthesis of infectious RNA from cloned brome mosaic virus cDNA and effects of 5′ extensions on transcript infectivity. Virology 158:259-262.
23. Kamer, G., and P. Argos. 1984. Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Res. 12:7269-7282.
24. Kao, C. C., and P. Ahlquist. 1992. Identification of the domains required for the direct interaction of the helicase-like and polymerase-like RNA replication proteins of brome mosaic virus. J. Virol. 66:7293-7302.
25. Kao, C. C., R. Quadt, R. P. Hershberger, and P. Ahlquist. 1992. Brome mosaic virus RNA replication proteins 1a and 2a form a complex in vitro. J. Virol. 66:6322-6329.
26. Kiberstis, P. A., L. S. Loesch-Fries, and T. C. Hall. 1981. Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804-808.
27. Koonin, E. V., and V. V. Dolja. 1993. Evolution and taxonomy of positive-strand RNA viruses: implications and comparative analysis of amino acid sequences. Crit. Rev. Biochem. Mol. Biol. 28:375-430.
28. Kroner, P., and P. Ahlquist. 1992. RNA-based viruses, p. 23-34. In S. J. Gurr, M. J. McPherson, and D. J. Bewies (ed.), Molecular plant pathology: a practical approach, vol. 1. IRL Press at Oxford University Press, Oxford.
29. Kroner, P. A., B. M. Young, and P. Ahlquist. 1990. Analysis of the role of brome mosaic virus 1a protein domains in RNA replication, using linker insertion mutagenesis. J. Virol. 64:6110-6120.
30. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:367-382.
31. Lenim, J. A., T. Rümenapf, E. G. Strauss, J. H. Strauss, and C. M. Rice. 1994. Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis. EMBO J. 13:2925-2934.
32. Miller, W. A., T. W. Dreher, and T. C. Hall. 1985. Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (-)-sense genomic RNA. Nature (London) 313:68-70.
33. Mise, K., and P. Ahlquist. 1995. Host-specificity restriction by tiromovirus cell-to-cell movement protein occurs after initial cell-to-cell spread of infection in non-host plants. Virology 206:276-286.
34. O'Reilly, E. K., N. Tang, P. Ahlquist, and C. C. Kao. 1995. Biochemical and genetic analyses of the interaction between the helicase-like and polymerase-like proteins of the brome mosaic virus. Virology 214:59-71.
35. Pacha, R. F., and P. Ahlquist. 1992. Substantial portions of the 5′ and intercistronic regions of cowpea chlorotic mottle virus RNA3 arc dispensable for systemic infection but influence viral competitiveness and infection pathology. Virology 187:298-307.
36. Quadt, R., and E. M. J. Jaspars. 1990. Purification and characterization of brome mosaic virus RNA-dependent RNA polymerase. Virology 178:189-194.
37. Quadt, R., H. J. M. Verbeek, and E. M. J. Jaspars. 1988. Involvement of a nonstructural protein in the RNA synthesis of brome mosaic virus. Virology 165:256-261.
38. Rao, A. L. N., and T. C. Hall. 1990. Requirement for a viral trans-acting factor encoded by brome mosaic virus RNA-2 provides strong selection in vivo for functional recombinants. J. Virol. 64:2437-2441.
39. Romero, J., A. M. Dzianott, and J. J. Bujarski. 1992. The nucleotide sequence and genome organization of the RNA2 and RNA3 segments in broad bean mottle virus. Virology 187:671-681.
40. Rozanov, M. N., E. V. Koonin, and A. E. Gorbalenya. 1992. Conservation of the putative methyltransferase domain: a hallmark of the 'Sindbis-like' supergroup of positive-strand RNA viruses. J. Gen. Virol. 73:2129-2134.
41. Scharf, S. J., G. T. Horn, and H. A. Erlich. 1986. Direct cloning and sequence-analysis of enzymatically amplified genomic sequence. Science 233:1076-1078.
42. Shirako, Y., and J. H. Strauss. 1994. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plusstrand synthesis. J. Virol. 68:1874-1885.
43. Skrjabin, K. G., S. Y. Morozov, A. S. Kraev, M. N. Rozanov, B. K. Chernov, L. I. Lukasheva, and J. G. Atabekov. 1988. Conserved and variable elements in RNA genomes of potexviruses. FEBS Lett. 240:33-40.
44. Skuzeski, J. M., L. M. Nichols, and R. F. Gesteland. 1990. Analysis of leaky viral translation termination codons in vivo by transient expression of improved β-glucuronidase vectors. Plant Mol. Biol. 15:65-79.
45. Skuzeski, J. M., L. M. Nichols, R. F. Gesteland, and J. F. Atkins. 1991. The signal for a leaky UAG stop codon in several plant viruses includes the two downstream codons. J. Mol. Biol. 218:365-373.
46. Strauss, J. H., and E. G. Strauss. 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58:491-562.
47. Töpfer, R., C. Maas, C. Höricke-Grandpierre, J. Shell, and H. H. Steinbiss. 1993. Expression vectors for high-level gene expression in dicotyledonous and monocotyledonous plants. Methods Enzymol. 217:66-78.
48. Töpfer, R., V. Matzeit, B. Gronenbourn, J. Shell, and H. H. Steinbiss. 1987 A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Res. 15:5890.
49. Traynor, P., and P. Ahlquist. 1990. Use of bromovirus RNA2 hybrids to map cis- and trans-acting functions in a conserved RNA replication gene. J. Virol. 64:69-77.
50. Traynor, P., B. M. Young, and P. Ahlquist. 1991. Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread. J. Virol. 65:2807-2815.