The design of an alternative, covalently flavinylated 6-hydroxy-D-nicotine oxidase by replacing the FAD-binding histidine by cysteine and reconstitution of the holoenzyme with 8-(methylsulfonyl)FAD

FEBS Letters

Volumn 386, Issue 2-3, 1996, Pages 194-196

  Stoltz, Michaela ^a   Henninger, Hans Peter ^a   Brandsch, Roderich ^a  

^a Inst fur Biochem/Molekularbiologie   (Germany)

Author keywords

Covalent flavinylation; FAD; Flavoenzyme; Site directed mutagenesis

Indexed keywords

6 HYDROXYNICOTINE OXIDASE; FLAVINE ADENINE NUCLEOTIDE; FLAVOPROTEIN; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME BINDING; ESCHERICHIA COLI; GENE EXPRESSION; MUTANT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SPECTROPHOTOMETRY;

ESCHERICHIA COLI;

EID: 0029900462     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(96)00438-3     Document Type: Article

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