Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes

European Journal of Biochemistry

Volumn 239, Issue 3, 1996, Pages 726-731

  Meyer, Christiane ^a   Schmid, Roland ^b   Scriba, Peter C ^a   Wehling, Martin ^a,c  

^a UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Amino acid sequence; Liver; Membrane binding site; Progesterone

Indexed keywords

BINDING PROTEIN; PROGESTERONE; STEROID RECEPTOR;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BINDING SITE; CONTROLLED STUDY; LIVER MEMBRANE; LIVER MICROSOME; MEMBRANE BINDING; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SOLUBILIZATION; SWINE;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 0029900104     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0726u.x     Document Type: Article

References (39)

1. Nemere, I., Zhou, L.-X. & Norman, A. W. ( 1993) Nontranscriptional effects of steroid hormones. Receptor 3, 277-291.
2. Wehling, M. (1995) Looking beyond the dogma of genomic steroid action: insights and facts of the 1990s, J. Mol. Med. 73, 439 447.
3. 2+]III of Xenopus laevis oocytes, Proc. Natl Acad. Sci. USA 77, 1534-1536.
4. Blackmore, P. F., Beebe, S. J., Danforth, R. D. & Alexander, N. (1990) Novel stimulators of calcium influx in human sperm, J. Biol Chem. 265, 1376-1380.
5. 2+ and the acrosome reaction in human sperm. Molecular Signalling 5, 531-538.
6. Turner, K. O. & Meizel, S. (1995) Progesterone-mediated efflux of cytosolic chloride during the human sperm acrosome reaction. Biochem. Biophys. Res. Commun. 213, 774-780.
7. Waldegger, S., Beisse, F., Apfel, H., Breit, S., Kolb, H.-A., Häussinger, D. & Lang, F. (1995) Electrophysiological effects of progesterone on hepatocytes, Biochim. Biophys. Acta 1266, 186-190.
8. Wehling, M., Käsmayr, J. & Theisen, K. (1991) Rapid effects of mineralocorticoids on sodium-proton exchanger: genomic or nongenomic pathway? Am. J. Physiol. 261, E719-E726.
9. Christ, M., Douwes, K., Eisen, C., Bechtner, G., Theisen, K. & Wehling, M. (1995) Rapid effects of aldosterone on sodium transport in vascular smooth muscle cells, Hypertension 25, 117-123.
10. Christ, M., Eisen, C., Aktas, J., Theisen, K. & Wehling, M. (1993) The inositol-1,4,5-trisphosphate system is involved in rapid effects of aldosterone in human mononuclear leukocytes, J. Clin. Endocrinol. Metab. 77, 1452-1457.
11. Christ, M., Meyer, C., Sippel, K. & Wehling, M. (1995) Rapid aldosterone signaling in vascular smooth muscle cells: involvement of the phospholipase C, diacylglycerol and protein kinase C112. Biochem. Biophys. Res. Commun, 213, 123-129.
12. Wehling, M., Ulsenheimer, A., Schneider, M., Neylon, C. & Christ, M. (1994) Rapid effects of aldosterone on free intracellular calcium in vascular smooth muscles and endothelial cells: subcellular localization of calcium elevation by single cell imaging. Biochem. Biophys. Res. Commun. 204, 475-481.
13. Wehling, M. (1994) Specificity-conferring mechanism at the level of the cell membrane, Steroids 59, 160-163.
14. Suyemitsu, T. & Terayama, H. (1975) Specific binding sites for natural glucocorticoids in plasma membranes of rat liver, Endocrinology 96, 1499-1508.
15. Ibarolla, I., Alejandro, A., Marino, A., Sancho, M. J., Macarulla, J. M. & Trueba, M. (1992) Characterization by photoaffinity labeling of a steroid binding protein in rat liver plasma membrane, J. Membrane biol. 125, 185-191.
16. Yamada, M. & Miyaji, H. (1982)Binding of sex hormones by male rat liver microsomes, J. Steroid Biochem. 16, 437-446.
17. Trueba. M., Rodriguez, P., Vallejo, A. I., Marino, A., Sancho, M. J. & Macarulla, J. M. (1990) Binding of progesterone to specific sites in isolated hepatic cells and purified plasma membrane fraction, Exp. Clin. Endocrinol. 95, 169-180.
18. Pietras, R. J. & Szego, C. M. (1980) Partial purification and characterization of oestrogen receptors in subtractions of hepatocyte plasma membranes, Biochem. J. 191, 743-760.
19. 3H]Dexamethasone binding to plasma membrane-enriched fractions from liver of nonadrenalectomized rats. Endocrinology 123, 1642-1651.
20. Wright, M. C. & Paine, A. J. (1995) Characterization of a membrane-associated steroid binding site in rat liver, J. Recept. Signal Transduct. Res. 15, 543-556.
21. Trueba, M., Ibarolla, I., Vallejo, A. I., Sancho, M. J., Marino, A. & Macarulla, J. M. (1989) Characterization of specific binding sites for corticosterone in mouse liver plasma membrane, Membr. Biochem. 8, 229-239.
22. Trueba, M., Ibarolla, I., Ogiza, K., Marino, A. & Macarulla, J. M. (1991) Specific binding sites for corticosterone in isolated cells and plasma membrane from rat liver. J. Membrane Biol. 120, 115-124.
23. Meyer, C., Christ, M. & WehlinG, M. (1995) Characterization and solubilization of novel aldosterone-binding-proteins in procine liver microsomes, Eur. J. Biochem. 229, 736-740.
24. Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
25. Bruns, R. F., Lawson-Wendling, K. & Pugsley, T. A. (1983) A rapid filtration assay for soluble receptors using polyethyleniminetreated filters, Anal. Biochem. 132, 74-81.
26. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
27. Blum, H., Beier, H. & Groß, H. J. (1987) Improved silver stained of plant proteins, RNA and DNA in polyacrylamide gels, Electrophoresis 8, 93-99.
28. Leatherbarrow, R. J. (1987) Enzfitter program, Elsevier Biosoft, Cambridge UK.
29. Revelli, A., Modotti, M., Piffaretti-Yanez, A., Massobrio, M. & Balerna, M. (1994) Steroid receptors in human spermatozoa, Hum. Reprod. (Oxf.) 9, 760-766.
30. Wehling, M. (1994) Nongenomic actions of steroid hormones, Trends Endocrinol. Metab. 5, 347-353.
31. Liu, Z. & Patiño, R. (1993) High-affinity binding of progesterone to the plasma membrane of Xenopus oocytes: Characteristics of binding and hormonal and developmental control, Biol. Reprod. 49, 980-988.
32. Sadler, S. E. & Maller, J. L. (1982) Identification of a steroid receptor on the surface of Xenopus oocytes by photoaffinity labeling, J. Biol. Chem. 257, 355-361.
33. Haukkamaa, M. (1976)Progesterone-binding properties of microsomes from rat liver, J. Steroid Biochem. 7, 345-348.
34. 125Ibovine serum albumin as a ligand, J. Neurochem. 54, 467-472.
35. Tischkau, S. A. & Ramirez, V. D. (1993) A specific membrane binding protein for progesterone in rat brain: Sex differences and induction by estrogen, Proc. Natl Acad. Sci. USA 90, 1285-1289.
36. Humle, E. C. (1990) Receptor-effector coupling, a practical approach, IRL Press, Oxford.
37. Riad-Fahmy, D., Read, G. F., Walker, R. F. & Griffiths, K. (1982) Steroids in saliva for assessing endocrine functions, Endocrine Rev. 3, 367-395.
38. 125I]Iodohistamine) identifies four protein bands in mouse brain membranes, J. Neurochem. 63, 1434-1438.
39. Blondeau, J.-P. & Baulieu, E.-E. (1984) Progesterone receptors characterized by photoaffinity labelling in the plasma membrane of Xenopus oocytes, Biochem. J. 219, 785-792.