Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis

Journal of Cell Biology

Volumn 133, Issue 6, 1996, Pages 1347-1353

  Funakoshi, Takeshi ^a   Takeda, Sen ^a   Hirokawa, Nobutaka ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

TUBULIN;

ACTIVE TRANSPORT; ANIMAL CELL; ARTICLE; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; MICROTUBULE; MOUSE; NERVE FIBER GROWTH; NONHUMAN; PHOTOACTIVATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TRANSPORT;

ANIMALS; AXONAL TRANSPORT; AXONS; BIOLOGICAL TRANSPORT, ACTIVE; CELL MEMBRANE PERMEABILITY; FLUORESCEINS; FLUORESCENT DYES; MICE; MICROSCOPY, IMMUNOELECTRON; MICROTUBULES; POLYMERS; PROTEIN CONFORMATION; SWINE; TUBULIN; ULTRAVIOLET RAYS;

ANIMALIA;

EID: 0029898806     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.133.6.1347     Document Type: Article

References (39)

1. Ahmad, F.J., and P.W. Baas. 1995. Microtubules released from the neuronal centrosome are transported into the axon. J. Cell Sci. 108:2761-2769.
2. Ahmad, F.J., H.C. Joshi, V.E. Centonze, and P.W. Baas. 1994. Inhibition of microtubule nucleation at the neuronal centrosome compromises axon growth. Neuron. 12:271-280.
3. Baas, P.W., and M.M. Black. 1990. Individual microtubules in the axon consist of domains that differ in both composition and stability. J. Cell Biol. 111:495-509.
4. Baas, P.W., and F.J. Ahmad. 1993. The transport properties of axonal microtubules establish their polarity orientation. J. Cell Biol. 120:1427-1437.
5. Bamburg, J.R., D. Bray, and K. Chapman. 1986. Assembly of microtubules at the tip of growing axons. Nature (Lond.). 321:788-790.
6. Brady, S.T. 1995. A kinesin medley: biochemical and functional heterogeneity. Trends Cell Biol. 5:159-164.
7. Black, M.M., and R.J. Lasek. 1980. Slow components of axonal transport: two cytoskeletal networks. J. Cell Biol. 86:616-623.
8. Cleveland, D.W., and P.N. Hoffman. 1991. Slow axonal transport models come full circle: evidence that microtubule sliding mediates axon elongation and tubulin transport. Cell. 67:453-456.
9. Glass, J.D., and J.W. Griffin. 1994. Retrograde transport of radiolabeled cyloskeletal proteins in transected nerves. J. Neurosci. 14:3915-3921.
10. Grafstein, B., and D.S. Forman. 1980. Intracellular transport in neurons. Phys-iol. Rev. 60:1167-1283.
11. Hayat, MA. 1989. Principles and techniques of electron microscopy. CRC Press, Boca Raton, FL. 240-242.
12. Hirokawa, N. 1993. Axonal transport and the cyloskeleton. Curr. Opin. Neurobiol. 3:724-731.
13. Hirokawa, N. 1996. Organelle transport along microtubules - the role of KIFs. Trends Cell Biol. 6:135-141.
14. Hirokawa, N., and S. Okabe. 1992. Microtubules on the Move? Curr. Biol. 2:193-195.
15. Hoffman, P.N., M.A. Lopata, D.F. Watson, and R.F. Luduena. 1992. Axonal transport of class II and III β-tubulin: evidence that the slow component wave represents the movement of only a small fraction of the tubulin in mature motor axons. J. Cell Biol. 119:595-604.
16. Hollenbeck, P.J. 1989. The transport and assembly of the axonal cytoskeleton. J. Cell Biol. 108:223-227.
17. Hollenbeck, P.J. 1990. Cytoskeleton on the move. Nature (Lond.). 343:408-409.
18. Joshi, H.C., and P.W. Baas. 1993. A new perspective on microtubules and axon growth. J. Cell Biol. 121:1191-1196.
19. Lasek, R.J. 1986. Polymer sliding in axons. J. Cell Sci. Suppl. 5:161-179.
20. Lasek, R.J., J.A. Garner, and S.T. Brady. 1984. Axonal transport of the cytoplasmic matrix. J. Cell Biol. 99:212s-221s.
21. Lim, S.S., K.J. Edson, P.C. Letourneau, and G.G. Borisy. 1990. A test of microtubule translocation during neurite elongation. J. Cell Biol. 111:123-130.
22. Mitchison, T.J. 1989. Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 109:637-652.
23. Nixon, R.A. 1991. Axonal Transport of Cytoskeletal Proteins. In The Neuronal Cytoskeleton. R.D. Burgoyne, editor. Wiley-Liss, Inc. pp. 283-303.
24. Nixon, R.A. 1992. Slow axonal transport. Curr. Opin. Cell Biol. 4:8-14.
25. Nixon, R.A., and K.B. Logvinenko. 1986. Multiple fates of newly synthesized neurofilament proteins: evidence for a stationary neurofilament network distributed nonuniformly along axons of retinal ganglion cell neurons. J. Cell Biol. 102:647-659.
26. Okabe, S., and N. Hirokawa. 1988. Microtubule dynamics in nerve cells: analysis using microinjection of biotinylated tuhulin into PC12 cells. J. Cell Biol. 107:651-664.
27. Okabe, S., and N. Hirokawa. 1990. Turnover of fluorescently labeled tubulin and actin in the axon. Nature (Lond.). 343:479-482.
28. Okabe, S., and N. Hirokawa. 1991. Actin dynamics in growth cones. J. Neurosci. 11:1918-1929.
29. Okabe, S., and N. Hirokawa. 1992. Differential behavior of photoactivated microtubules in growing axons of mouse and frog neurons. J. Cell Biol. 117:105-120.
30. Okabe, S., and N. Hirokawa. 1993. Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon. J. Cell Biol. 120:1177-1186.
31. Popov, S., and M. Poo. 1992. Differential transport of macromolecules in developing nerve processes. J. Neurosci. 12:77-85.
32. Reinsch, S.S., T.J. Mitchison, and M. Kirschner. 1991. Microtubule polymer assembly and transport during axonal elongation. J. Cell Biol. 115:365-379.
33. Salmon, E.D., W.M. Saxton, R.J. Leslie, M.L. Karow, and J.R. McIntosh. 1984. Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching. J. Cell Biol. 99:2157-2164.
34. Sabry, J., T.P. O'Connor, and M.W. Kirschner. 1995. Axonal transport of tubulin in Ti1 pioneer neurons in situ. Neuron. 14:1247-1256.
35. Stromka, D.P., and S. Ochs. 1981. Patterns of slow transport in sensory nerves. J. Neurobiol. 12:441-453.
36. Takeda, S., T. Funakoshi, and N. Hirokawa. 1995. Tubulin dynamics in neuronal axons of living zebrafish embryos. Neuron. 14:1257-1264.
37. Tashiro, T., and Y. Komiya. 1989. Stable and dynamic forms of cytoskelctal proteins in slow axonal transport. J. Neurosci. 9:760-768.
38. Umeyama, T., S. Okabe, Y. Kanai, and N. Hirokawa. 1993. Dynamics of microtubules bundled by microtubule associated protein 2C (MAP2C). J. Cell Biol. 120:451-465.
39. Yu, W., V.E. Centonze, F.J. Ahmad, and P.W. Baas. 1993. Microtubule nucleation and release from the neuronal centrosome. J. Cell Biol. 122:349-359.