PCR with random primers to obtain sequence from yeast artificial chromosome insert ends or plasmids

BioTechniques

Volumn 20, Issue 3, 1996, Pages 486-491

  Swensen, Jeff ^a,b  

^a UNIVERSITY OF UTAH   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; OLIGONUCLEOTIDE;

ARTICLE; DELETION MUTANT; GENE MAPPING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; YEAST ARTIFICIAL CHROMOSOME;

EID: 0029897668     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003486     Document Type: Article

References (9)

1. Green, E.D. and M.V. Olson. 1990. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping. Science 250:94-98.
2. Henikoff, S. 1984. Unidirectional digestion with exonuclease III creates targeted break-points for DNA sequencing. Gene 28:351-359.
3. Liu, Y.-G. and R.F. Whittier. 1995. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681.
4. Miki, Y., J. Swensen, D. Shattuck-Eidens, P.A. Futreal, K. Harshman, S. Tavtigian, Q. Liu, C. Cochran, et al. 1994. Isolation of a strong candidate for the 17q-linked breast and ovarian cancer susceptibility gene, BRCA1. Science 206:66-71.
5. Nelson, D.L., A. Ballablo, M.F. Victoria, M. Pieretti, R.D. Bies, R.A. Gibbs et al. 1991. Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: Identification of clones associated with a disease locus. Proc. Natl. Acad. Sci. USA 88:6157-6161.
6. Neuhausen, S.L., J. Swensen, Y. Miki, Q. Liu, S. Tavtigian, D. Shattuck-Eidens et al. 1994. A P1-based physical map of the region from D17S776 to D17S78 containing the breast cancer susceptibility gene BRCA1. Hum. Mol. Genet. 3:1919-1926.
7. Olson, M.V., J.E. Dutchik, M.Y. Graham, G.M. Brodeur, C. Helms, M. Frank et al. 1986. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83:7826-7830.
8. Pierce, J.C., B. Sauer and N. Sternberg. 1992. A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: Improved cloning efficacy. Proc. Natl. Acad. Sci. USA 89:2056-2060.
9. Silverman, G.A. 1993. Isolating vector-insert junctions from yeast artificial chromosomes. PCR Methods Appl. 3:141-150.