In vivo chromatin structure of the murine interleukin-5 gene region: A new intact cell system

BioTechniques

Volumn 20, Issue 5, 1996, Pages 834-840

  Ymer, Sanie ^a,b   Jans, David A ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 5; LYSOPHOSPHATIDYLCHOLINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHROMATIN STRUCTURE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; MOUSE; NONHUMAN; T LYMPHOCYTE;

ANIMALIA; MURINAE;

EID: 0029897511     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205st02     Document Type: Article

References (20)

1. Bourke, P.F., B.H. van Leeuwen, H.D. Campbell and I.G. Young. 1995. Localisation of the inducible enhancer in the mouse interleukin-5 gene which is responsive to T cell receptor stimulation. Blood 85:2069-2077.
2. Campbell, H.D., S. Ymer, M. Fung and I.G. Young. 1985. Cloning and nucleotide sequence of the murine interleukin-3 gene. Eur. J. Biochem. 150:297-304.
3. Contreras, R. and W. Fiers. 1981. Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CV-1 cells: evidence for multiple promoters of SV40 late transcription. Nucleic Acids Res. 2:215-236.
4. Elgin, S.C.R. 1988. The formation and function of DNase I hypersensitive sites in the process of gene activation. J. Biol. Chem 263:19259-19262.
5. Greenberg, M.E. and T.P. Bender. 1993. Identification of newly transcribed RNA, Section 4.10.1-4.10.11, Volume 1. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seideman, J.A. Smith and K.Struhl (Eds.), Current Protocols in Molecular Biology. Wiley Interscience, New York.
6. Jans, D.A. The regulation of protein transport to the nucleus by phosphorylation. Biochem. J. (In press).
7. 124 inhibits nuclear import of SV40 T-antigen proteins. J. Cell. Biol. 115:1203-1212.
8. 112 of the casein kinase II site flanking the nuclear localization signal of the SV40 large T-antigen is mechanistically important in enhancing nuclear import. Oncogene 9:2961-2968.
9. Kaye, J., S. Porcelli, J. Tite, B. Jones and C.A. Janeway. 1983. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158:836-856.
10. Leno, G.H., C.S. Downes and R.A. Laskey. 1992. The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract. Cell 69:151-158.
11. Miller, M.R., J.J. Castellot, Jr. and A.B Pardee. 1978. A permeable animal cell preparation for studying macromolecular synthesis. DNA synthesis and the role of deoxyribonucleotides in S phase initiation. Biochemistry 17:1073-1080.
12. Mueller, P.R. and B. Wold. 1989. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science 246:780-786.
13. Naora, H. and I.G. Young. 1994. Mechanisms regulating the mRNA levels of interleukin-5 and two other coordinately expressed lymphokines in the murine T lymphoma EL4.23. Blood 83:3620-3628.
14. Naora, H. and I.G. Young. 1995. Comparison of the mechanisms regulating IL-5, IL-4 and three other lymphokine genes in the Th2 clone D10.G4.1. Exp. Hematol. 23:597-602.
15. Pfeifer, G.P. and A.D. Riggs. 1991. Chromatin differences between active and inactive X chromosomes revealed by genomic foot-printing of permeabilized cells using DNase I and ligation-mediated PCR. Genes Dev. 5:1102-1113.
16. Rihs, H-P., D.A. Jans, H. Fan and R. Peters. 1991. The rate of nuclear cytoplasmic protein transport is determined by a casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen. EMBO J. 10:633-639.
17. Sekhar-Reddy, P.M. and C.J. Shen. 1991. Protein-DNA interactions in vivo of an erythroid-specific, human β-globin locus enhancer. Proc. Natl. Acad. Sci. USA 88:8676-8680.
18. Stewart, A.F., A. Reik and G. Schutz. 1991. A simpler and better method to cleave chromatin with DNase1 for hypersensitive site analyses. Nucleic Acid Res. 19:3157.
19. Wu, C. 1980. Analysis of hypersensitive sites in chromatin. Methods Enzymol. 170:269-289.
20. Zhang, L. and J.D. Gralla. 1989. In situ nucleoprotein structure at the SV40 major late promoter: melted and wrapped DNA flank the start site. Genes Dev. 3:1814-1822.