Detection of K-ras point mutations at codon 12 in pancreatic juice for the diagnosis of pancreatic cancer by hybridization protection assay: A simple method for the determination of the types of point mutations

Japanese Journal of Cancer Research

Volumn 87, Issue 5, 1996, Pages 466-474

  Watanabe, Hiroyuki ^a   Miyagi, Chieko ^b   Yamaguchi, Yasushi ^a   Satomura, Yoshitake ^a   Ohta, Hideki ^a   Motoo, Yoshiharu ^a   Okai, Takashi ^a   Yoshimura, Tadashi ^b   Tsuji, Yasuhiro ^b   Sawabu, Norio ^a  

^a KANAZAWA UNIVERSITY   (Japan)

^b CHUGAI PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Acridinium ester; Genetic diagnosis; Hybridization protection assay; K ras mutation; Pancreatic cancer

Indexed keywords

ASPARTIC ACID; CYSTEINE;

ARTICLE; CLINICAL ARTICLE; CODON; DNA HYBRIDIZATION; FIBEROSCOPE; HUMAN; HUMAN CELL; ONCOGENE K RAS; PANCREAS CANCER; PANCREAS JUICE; POINT MUTATION; PRIORITY JOURNAL;

BASE SEQUENCE; CODON; DNA PROBES; GENES, RAS; HUMANS; MOLECULAR SEQUENCE DATA; PANCREATIC JUICE; PANCREATIC NEOPLASMS; POINT MUTATION; POLYMERASE CHAIN REACTION;

EID: 0029896557     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.1996.tb00247.x     Document Type: Article

References (34)

1. Nakaizumi, A., Tatsuta, M., Uehara, H., Yamamoto, R., Takenaka, A., Kishigami, Y., Takemura, K., Kitamura, T. and Okuda, S. Cytologic examination of pure pancreatic juice in the diagnosis of pancreatic carcinoma. Cancer, 70, 2610-2614 (1992).
2. Kameya, S., Kuno, N. and Kasugai, T. The diagnosis of pancreatic cancer by pancreatic juice cytology. Acta Cytol., 25, 354-360 (1981).
3. Almoguera, C., Shibata, D., Forrester, K., Martin, J., Arnheim, N. and Percho, M. Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes. Cell, 53, 549-554 (1988).
4. Smit, V. T. H. B. M., Boot, A. J. M., Fleuren, G. J., Cornelisse, C. J. and Bos, J. L. K-ras codon 12 mutations occur very frequently in pancreatic adenocarcinomas. Nucleic Acids Res., 16, 7773-7782 (1988).
5. Grünewald, K., Lyons, J., Fröhlich, A., Feichtinger, H., Weger, R. A., Schwab, G., Jassen, J. W. G. and Bartram, C. R. High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas. Int. J. Cancer, 43, 1037-1041 (1989).
6. Mariyama, M., Kishi, K., Nakamura, K., Obata, H. and Nishimura, S. Frequency and types of point mutation at the 12th codon of the c-Ki-ras gene found in pancreatic cancers from Japanese patients. Jpn. J. Cancer Res., 80, 622-626 (1989).
7. Nagata, Y., Abe, M., Motoshima, K., Nakayama, E. and Shiku, H. Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japanese. Jpn. J. Cancer Res., 81, 135-140 (1990).
8. Tada, M., Omata, M. and Ohto, M. Clinical application of ras gene mutation for diagnosis of pancreatic adenocarcinoma. Gastroenterology, 100, 233-238 (1991).
9. Watanabe, H., Sawabu, N., Ohta, H., Satomura, Y., Yamakawa, O., Motoo, Y., Okai, T., Takahashi, H. and Wakabayashi, T. Identification of K-ras oncogene mutations in the pure pancreatic juice of patients with ductal pancreatic cancers. Jpn. J. Cancer Res., 84, 961-965 (1993).
10. Watanabe, H., Sawabu, N., Songür, Y., Yamaguchi, Y., Yamakawa, O., Satomura, Y., Ohta, H., Motoo, Y., Okai, T. and Wakabayashi, T. Detection of K-ras point mutations at codon 12 in pure pancreatic juice for the diagnosis of pancreatic cancer by PCR-RFLP analysis. Pancreas, 12, 18-24 (1996).
11. Arnold, L. J., Hammond, P. W., Wiese, W. A. and Nelson, N. C. Assay formats involving acridinium-ester-labeled DNA probes. Clin. Chem., 35, 1588-1594 (1989).
12. Japan Pancreas Society ed. "General Rules for Cancer of the Pancreas" (1993). Kanehara Co., Tokyo (in Japanese).
13. Japanese Society of Gastroenterology ed. "Clinical Criteria of Chronic Pancreatitis" (1984). Igaku Tosho Co., Tokyo (in Japanese).
14. Takemori, Y., Sawabu, N., Ohta, H., Satomura, Y., Watanabe, H., Yamakawa, O., Kidani, H., Takahashi, H. and Wakabayashi, T. Evaluation of cancer-associated carbohydrate antigen (NCC-ST-439) measurement in pure pancreatic juice collected by endoscopic aspiration. Dig. Endosc., 2, 141-147 (1990).
15. Wakabayashi, T., Sawabu, N., Takemori, Y., Satomura, Y., Kidani, H., Ohta, H., Watanabe, H., Yamakawa, O., Takahashi, H., Watanabe, K. and Konishi, F. Diagnostic significance of cancer-associated carbohydrate antigen (CA19-9) concentrations in pancreatic juice collected by endoscopic aspiration and immunohistochemical study in chronic pancreatitis. Pancreas, 8, 151-158 (1993).
16. Ohta, H., Sawabu, N., Takemori, Y., Kidani, H., Wakabayashi, T., Satomura, Y., Watanabe, H., Motoo, Y., Okai, T. and Takahashi, H. Measurement of sialylated stage-specific embryonic antigen-1 in pure pancreatic juice for the diagnosis of pancreatic cancer. Int. J. Pancreatol., 15, 35-41 (1994).
17. Bowtell, D. D. L. Rapid isolation of eukaryotic DNA. Anal. Biochem., 162, 463-465 (1987).
18. Jeanpiérrè, M. A rapid method for the purification of DNA from blood. Nucleic Acids Res., 15, 9611 (1987).
19. Saiki, R. K., Scharf, S., Fallona, F., Mullis, K. B., Horn, G. T., Erlich, H. A. and Arnheim, N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350-1354 (1985).
20. Deng, W. P. and Nickoloff, J. A. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem., 200, 81-88 (1992).
21. Haliassos, A., Chomel, J. C., Grandjouan, S., Kruh, J., Kaplan, J. C. and Kitzis, A. Detection of minority point mutations by modified PCR technique: a new approach for a sensitive diagnosis of tumor-progression markers. Nucleic Acids Res., 17, 8093-8099 (1989).
22. Lerman, L. S. The structure of the DNA-acridine complex. Proc. Natl. Acad. Sci. USA, 49, 94-102 (1963).
23. Asseline, U., Delarue, M., Lancelot, G., Toulmé, F., Thuong, N. T., Montenay-Garestier, T. and Hélène, C. Nucleic acid-binding molecules with high affinity and base sequence specificity: intercalating agents covalently linked to oligodeoxynucleotides. Proc. Natl. Acad. Sci. USA, 81, 3297-3301 (1984).
24. Cieplak, P., Rao, S. N., Hélène, C., Montenay-Garestier, T. and Kollman, P. A. Conformations of duplex structures formed by oligodeoxynucleotides covalently linked to the intercalator 2-methoxy-6-chloro-9-aminoacridine. J. Biomol. Struct. Dyn., 5, 361-382 (1987).
25. Capella, G., Cronauer-Mitra, S., Peinado, M. A. and Perucho, M. Frequency and spectrum of mutations at codon 12 and 13 of the c-K-ras gene in human tumors. Environ. Health Perspect., 93, 125-131 (1991).
26. Hruban, R. H., Mansfeld, A. D. M., Offerhaus, G. J. A., Weering, D. H. J., Allison, D. C., Goodman, S. N., Kensler, T. W., Bose, K. K., Cameron, J. L. and Bos, J. L. K-ras oncogene activation in adenocarcinoma of the human pancreas: a study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization. Am. J. Pathol., 143, 545-554 (1993).
27. Tada, M., Omata, M., Kawai, S., Saisho, H., Ohto, M., Saiki, R. K. and Sninsky, J. J. Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Res., 53, 2472-2474 (1993).
28. Miki, H., Matsumoto, S., Harada, H., Mori, S., Haba, R., Ochi, K., Kobayashi, S. and Ohmori, M. Detection of c-Ki-ras point mutation from pancreatic juice. Int. J. Pancreatol., 14, 145-148 (1993).
29. Kondo, H., Sugano, K., Fukayama, N., Kyogoku, A., Nose, H., Shimada, K., Ohkura, H., Ohtsu, A., Yoshida, S. and Shimosato, Y. Detection of point mutation in the K-ras oncogene at codon 12 in pure pancreatic juice for diagnosis of pancreatic carcinoma. Cancer, 73, 1589-1594 (1994).
30. Yanagisawa, A., Ohtake, K., Ohhashi, K., Hori, M., Kitagawa, T., Sugano, H. and Kato, Y. Frequent c-Ki-ras oncogene activation in mucous cell hyperplasias of pancreas suffering from chronic inflammation. Cancer Res., 53, 953-956 (1993).
31. Caldas, C., Hahn, S. A., Hruban, R. H., Redston, M. S., Yeo, C. J. and Kern, S. E. Detection of K-ras mutations in the stool of patients with pancreatic adenocarcinoma and pancreatic ductal hyperplasia. Cancer Res., 54, 3568-3573 (1994).
32. Tada, M., Kawabe, T. and Omata, M. Diagnosis of pancreatic carcinoma by oncogene. Liver Gallbladder Pancreas, 30, 609-614 (1995) (in Japanese).
33. Tabata, T., Fujimori, T., Maeda, S., Yamamoto, M. and Saitoh, Y. The role of ras mutation in pancreatic cancer, precancerous lesions, and chronic pancreatitis. Int. J. Pancreatol., 14, 237-244 (1993).
34. Matsubara, K., Koide, Y., Kobayashi, A., Kaida, R., Takeda, S., Matsuda, E., Matsuoka, Y. and Yoshida, T. O. A rapid and sensitive method for HLA-DRB1 typing by acridinium-ester-labeled DNA probes. Hum. Immunol., 35, 132-139 (1992).