Role of the "helix clamp" in HIV-1 reverse transcriptase catalytic cycling as revealed by alanine-scanning mutagenesis

Journal of Biological Chemistry

Volumn 271, Issue 21, 1996, Pages 12213-12220

  Beard, William A ^a   Minnick, Dana T ^b   Wade, Cheryl L ^a   Prasad, Rajendra ^a   Won, Ran L ^a   Kumar, Amalendra ^a   Kunkel, Thomas A ^b   Wilson, Samuel H ^b  

^a UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^b NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS;

DNA; RNA DIRECTED DNA POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA BINDING; DNA HELIX; ENZYME CHEMISTRY; GENE REPLICATION; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; VIRUS REPLICATION;

ALANINE; BASE SEQUENCE; CATALYSIS; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RNA-DIRECTED DNA POLYMERASE; SUBSTRATE SPECIFICITY; THYMINE NUCLEOTIDES;

EID: 0029893953     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.21.12213     Document Type: Article

References (36)

1. Smerdon, S. J., Jager, J., Wang, J., Kohlstaedt, L. A., Chirino, A. J., Friedman, J. M., Rice, P. A., and Steitz, T. A. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 3911-3915
2. Ren, J., Esnouf, R., Garman, E., Somers, D., Ross, C., Kirby, I., Keeling, J., Darby, G., Jones, Y., Stuart, D., and Stammers, D. (1995) Nature Struct. Biol. 2, 293-302
3. Jacobo-Molina, A., Ding, J., Nanni, R. G., Clark, A. D., Jr., Lu, X., Tantillo, C., Williams, R. L., Kamer, G., Ferris, A. L., Clark, P., Hizi, A., Hughes, S. H., and Arnold, E. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6320-6324
4. Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A., and Steitz, T. A. (1992) Science 256, 1783-1790
5. Nanni, R. G., Ding, J., Jacobo-Molina, A., Hughes, S. H., and Arnold, E. (1993) Perspect. Drug Discov. Des. 1, 129-150
6. Hermann, T., Meier, T., Götte, M., and Heumann, H. (1994) Nucleic Acids Res. 22, 4625-4633
7. Beard, W. A., Stahl, S. J., Kim, H.-R., Bebenek, K., Kumar, A., Strub, M.-P., Becerra, S. P., Kunkel, T. A., and Wilson, S. H. (1994) J. Biol. Chem. 269, 28091-28097
8. Bebenek, K., Beard, W. A., Casas-Finet, J. R., Kim, H.-R., Darden, T. A., Wilson, S. H., and Kunkel, T. A. (1995) J. Biol. Chem. 270, 19516-19523
9. Abbotts, J., Bebenek, K., Kunkel, T. A., and Wilson, S. H. (1993) J. Biol. Chem. 268, 10312-10323
10. Bebenek, K., Abbotts, J., Wilson, S. H., and Kunkel, T. A. (1993) J. Biol. Chem. 268, 10324-10334
11. Wöhrl, B. M., Tantillo, C., Arnold, E., and Le Grice, S. F. J. (1995) Biochemistry 34, 5343-5350
12. Kunkel, T. A., Bebenek, K., and McClary, J. (1991) Methods Enzymol. 204, 125-139
13. Goel, R., Beard, W. A., Kumar, A., Casas-Finet, J. R., Strub, M.-P., Stahl, S. J., Lewis, M. S., Bebenek, K., Becerra, S. P., Kunkel, T. A., and Wilson, S. H. (1993) Biochemistry 32, 13012-13018
14. Roberts, J. D., Bebenek, K., and Kunkel, T. A. (1988) Science 242, 1171-1173
15. Beard, W. A., and Wilson, S. H. (1993) Biochemistry 32, 9745-9753
16. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
17. Reardon, J. E., and Miller, W. H. (1990) J. Biol. Chem. 265, 20302-20307
18. Kati, W. M., Johnson, K. A., Jerva, L. F., and Anderson, K. S. (1992) J. Biol. Chem. 267, 25988-25997
19. Reardon, J. E. (1992) Biochemistry 31, 4473-4479
20. Jaju, M., Beard, W. A., and Wilson, S. H. (1995) J. Biol. Chem. 270, 9740-9747
21. Bebenek, K., and Kunkel, T. A. (1995) Methods Enzymol. 262, 217-232
22. Richardson, J. S., and Richardson, D. C. (1990) in Principles and Patterns of Protein Conformation (Fasman, G. D., ed) pp. 1-98, Plenum Press, New York
23. Bebenek, K., Abbotts, J., Roberts, J. D., Wilson, S. H., and Kunkel, T. A. (1989) J. Biol. Chem. 264, 16948-16956
24. Pelletier, H., Sawaya, M. R., Kumar, A., Wilson, S. H., and Kraut, J. (1994) Science 264, 1891-1903
25. Metzger, W., Hermann, T., Schatz, O., Le Grice, S. F. J., and Heumann, H. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 5909-5913
26. Boyer, P. L., Tantillo, C., Jacobo-Molina, A., Nanni, R. G., Ding, J. P., Arnold, E., and Hughes, S. H. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 4882-4886
27. Patel, P. H., Jacobo-Molina, A., Ding, J. P., Tantillo, C., Clark, A. D., Jr., Raag, R., Nanni, R. G., Hughes, S. H., and Arnold, E. (1995) Biochemistry 34, 5351-5363
28. Misra, V. K., and Honig, B. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 4691-4695
29. Divita, G., Müller, B., Immendorfer, U., Gautel, M., Rittinger, K., Restle, T., and Goody, R. S. (1993) Biochemistry 32, 7966-7971
30. Divita, G., Rittinger, K., Geourjon, C., Deleage, G., and Goody, R. S. (1995) J. Mol. Biol. 245, 508-521
31. Baillon, J. G., Nashed, N. T., Kumar, A., Wilson, S. H., and Jerina, D. M. (1991) New Biol. 3, 1015-1019
32. Rodgers, D. W., Gamblin, S. J., Harris, B. A., Ray, S., Culp, J. S., Hellmig, B., Woolf, D. J., Debouck, C., and Harrison, S. C. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1222-1226
33. Wang, J., Smerdon, S. J., Jager, J., Kohlstaedt, L. A., Rice, P. A., Friedman, J. M., and Steitz, T. A. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 7242-7246
34. Latham, G. J., and Lloyd, R. S. (1994) J. Biol. Chem. 269, 28527-28530
35. Seeman, N. C., Rosenberg, J. M., and Rich, A. (1976) Proc. Natl. Acad. Sci. U. S. A. 73, 804-808
36. Spolar, R. S., and Record, M. T. (1994) Science 263, 777-784