Thermostable variants of subtilisin selected by temperature-gradient gel electrophoresis

Electrophoresis

Volumn 17, Issue 4, 1996, Pages 784-792

  Sättler, Andrea ^a,b   Kanka, Susanne ^a   Maurer, Karl Heinz ^b   Riesner, Detlev ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b Werk Neynaber Chemie GmbH   (Germany)

Author keywords

Calcium binding site; Gel electrophoresis; Random mutagenesis; Screening; Temperature gradient; Thermal stability

Indexed keywords

CALCIUM; ELECTROPHORESIS; MUTAGENESIS; THERMODYNAMIC STABILITY;

BINDING-SITES; CALCIUM BINDING; CALCIUM BINDING SITE; GEL ELECTROPHORESIS; HEAT STABILITY; RANDOM MUTAGENESIS; RESIDUAL ACTIVITY; SUBTILISIN; SUBTILISIN CARLSBERG; TEMPERATURE GRADIENT GEL ELECTROPHORESIS;

THERMAL GRADIENTS;

ENZYME VARIANT; SUBTILISIN;

ARTICLE; AUTOLYSIS; BACILLUS SUBTILIS; BINDING SITE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ISOLATION; GEL ELECTROPHORESIS; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; POINT MUTATION; PROTEIN FOLDING; STRUCTURE ACTIVITY RELATION; THERMOSTABILITY;

EID: 0029892706     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150170428     Document Type: Article

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