Purification and characterization of 2-oxoglutarate: Ferredoxin oxidoreductase from a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6

Journal of Bacteriology

Volumn 178, Issue 11, 1996, Pages 3365-3368

  Yoon, K I Seok ^a   Ishii, Masaharu ^a   Igarashi, Yasuo ^a   Kodama, Tohru ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2 DIETHYLAMINOETHANOL; 2 OXOGLUTARIC ACID; AMMONIUM SULFATE; BACTERIAL ENZYME; COENZYME A; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PYRUVATE SYNTHASE; SEPHAROSE;

ARTICLE; DISSOCIATION CONSTANT; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBUNIT; HYDROGENOBACTER THERMOPHILUS; ISOLATION PROCEDURE; PH MEASUREMENT; PRIORITY JOURNAL; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

EID: 0029891269     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.11.3365-3368.1996     Document Type: Article

References (20)

1. Antranikian, G., C. Herzberg, and G. Gottschalk. 1982. Characterization of ATP citrate lyase from Chlorobium limicola. J. Bacteriol. 152:1284-1287.
2. Beh, M., G. Strauss, R. Huber, K. O. Stetter, and G. Fuchs. 1993. Enzymes of the reductive citric acid cycle in the autotrophic eubacterium Aquifex pyrophilus and in the archaebacterium Thermoproteus neutrophilus. Arch. Microbiol. 160:306-311.
3. Blamey, J. M., and M. W. W. Adams. 1993. Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. Biochim. Biophys. Acta 116:19-27.
4. Blamey, J. M., and M. W. W. Adams. 1994. Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima. Biochemistry 33:1000-1007.
5. Davis, B. J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121:404-427.
6. Gehring, U., and D. I. Arnon. 1972. Purification and properties of α-ketoglutarate synthase from a photosynthetic bacterium. J. Biol. Chem. 247: 6963-6969.
7. Ishii, M., Y. Igarashi, and T. Kodama. 1989. Purification and characterization of ATP:citrate lyase from Hydrogenobacter thermophilus TK-6. J. Bacteriol. 171:1788-1792.
8. Ishii, M., T. Kawasumi, V. Igarashi, T. Kodama, and Y. Minoda. 1987. 2-Methylthio-1,4-naphthoquinone, a unique sulfur-containing quinone from a thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus. J. Bacteriol. 169:2380-2384.
9. Kawasumi, T., Y. Igarashi, T. Kodama, and Y. Minoda. 1984. Hydrogenobacter thermophilus gen. nov., sp. nov., an extremely thermophilic, aerobic, hydrogen-oxidizing bacterium. Int. J. Syst. Bacteriol. 34:5-10.
10. Kerscher, L., and D. Oesterhelt. 1981. Purification and properties of two 2-oxoacid:ferredoxin oxidoreductases from Halobacterium halobium. Eur. J. Biochem. 116:587-594.
11. Kryukov, V. R., N. D. Savel'eva, and M. A. Pusheva. 1983. Calderobacterium hydrogenophilum gen. et sp. nov., an extremely thermophilic hydrogen bacterium and its hydrogenase activity. Microbiology (New York) 52:611-618.
12. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
13. Lineweaver, H., and D. Burk. 1934. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56:658-666.
14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
15. Matsudaira, P. 1987. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262: 10035-10038.
16. Schauder, R., F. Widdel, and G. Fuchs. 1987. Carbon assimilation pathways in sulfate reducing bacteria. II. Enzymes of a reductive citric acid cycle in the autotrophic Desulfobacter hydrogenophilus. Arch. Microbiol. 148:218-225.
17. 2 assimilation via the reductive tricarboxylic acid cycle in an obligately autotrophic, aerobic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus. Arch. Microbiol. 141:198-203.
18. Wahl, R. C., and W. H. Orme-Johnson. 1987. Clostridial pyruvate oxidoreductase and the pyruvate-oxidizing enzyme specific to nitrogen fixation in Klebsiella pneumoniae are similar enzymes. J. Biol. Chem. 262:10489-10496.
19. Yoon, K. S. 1996. Ph.D. thesis. University of Tokyo, Tokyo.
20. 1 Compounds, San Diego, California.