Simple, sensitive microplate IRMAs for intact and Des31,32-proinsulin compared with HPLC and cellulose IRAIAs

Clinical Chemistry

Volumn 42, Issue 6 SUPPL., 1996, Pages 977-979

  Mohamed Ali, Vidya ^a   Nagi, Dinesh K ^a   Yudkin, John S ^a  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DES 31,32 PROINSULIN; INSULIN DERIVATIVE; PROINSULIN; UNCLASSIFIED DRUG;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC VALUE; DIET RESTRICTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNORADIOMETRIC ASSAY; NON INSULIN DEPENDENT DIABETES MELLITUS; ORAL GLUCOSE TOLERANCE TEST;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIABETES MELLITUS, TYPE 2; HUMANS; IMMUNORADIOMETRIC ASSAY; PROINSULIN; PROTEIN PRECURSORS; REFERENCE VALUES;

EID: 0029891255     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/42.6.977     Document Type: Article

References (6)

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2. Dhahir F, Cook DB, Self CH. An amplified enzyme-linked immunoassay for human proinsulin. Clin Chem 1992;38:227-32.
3. Lundkjerns L, Røder ME, Dinesen B, Hartling SG, Jørgensen PN, Binder C. Highly sensitive enzyme immunoassay of proinsulin immunoreactivity with use of two monoclonal antibodies. Clin Chem 1993;10:2146-50.
4. Sobey WJ, Beer SF, Carrington CA, Clark PMS, Frank BH, Gray IP, et al. Sensitive and specific two-site immunoradiometric assays for human insulin, proinsulin, 65-66 split, and 32-33 split proinsulin. Biochem J 1989; 260:535-41.
5. Ostrega D, Polonski K, Nagi DK, Yudkin J, Cox LJ, Clark PMS, Hales CN. Measurement of proinsulin and intermediates: validation of immunoassay methods by high-performance liquid chromatography. Diabetes 1995;44: 437-40.
6. Mohamed-Ali V, Gould MM, Goubet S, Yudkin JS. The relationship between insulin, intact proinsulin and des 31,32 proinsulin and other cardiovascular risk factors in non-diabetics [Abstract]. Diabet Med 1993;10(Suppl 3):P54.