Microbial reduction of 2-(6-m-methoxyphenyl-3-oxohexyl)-2,4,5-trimethylcyclopenta-1,3-dione with Schizosaccharomyces pombe (NRRL Y-164)

Tetrahedron Asymmetry

Volumn 7, Issue 6, 1996, Pages 1563-1566

  Lee, Shoei Sheng ^a   Lin, Gwo Sen ^a   Wang, K C ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

2 (6 METHOXYPHENYL 3 OXOHEXYL) 2,4,5 TRIMETHYLCYCLOPENTA 1,3 DIONE; CYCLOPENTANONE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RACEMIC MIXTURE; REDUCTION; SCHIZOSACCHAROMYCES POMBE; SYNTHESIS;

EID: 0029889738     PISSN: 09574166     EISSN: None     Source Type: Journal    
DOI: 10.1016/0957-4166(96)00181-4     Document Type: Article

References (12)

1. 3OD using solvent peak as reference standard. 2D NMR spectra were recorded by using Bruker's standard pulse program: in the HMQC and HMBC experiments, Δ= 1 s and J =140, 8 Hz, respectively, the correlation maps consisted of 512× 1K data points per spectrum, each composed of 16 to 64 transients.
2. Wang, K.C.; Liang, Chang-Hsing; Kan, Wai-Ming; Lee, Shoei-Sheng. Bioorg & Med.Chem. 1994, 2, 27.
3. Schick, H.; Lehman, G.; Hilgetag. Chem. Ber. 1969,102, 3238.
4. +141 (100), 73 (91), 57 (84).
5. + 344 (32), 261 (28), 177 (51), 169 (73), 154 (30), 141 (37), 134 (100), 121 (48).
6. The microorganism was maintained on a MP-#3 Agar (Maltose 4%, Proteose Peptone #3 1.5%, and Agar 3%) at 26° C for 11 days then transferred and grown in Nutrient broth-Dextrose medium ( Nutrient broth 1.6% and Dextrose 4%) at 24-26° C on a rotary shaker (250 rpm, 1-in stroke). Transformation was carried out in 2-L Erlenmyer flasks containing 400 mL of medium. The substrate dissolved in DMF was added to the growing microorganism and the incubation was continued for 60 h.
7. + 346 (36), 328 (18), 212 (42), 197 (54), 177 (54), 142 (72), 121 (100).
8. Bowden, K.; Heilbron, I.M.; Jones, E.R.H.; Weedon, B.C.L. J. Chem. Soc. 1946, 39.
9. Wang, K.C. (Syntex Corp): Mikrobiologische Reduktion, Deutches Offenlengungsshift, 1971, 2,038,926.
10. + 346 (18), 331 (3), 212 (2), 188 (5) 162 (27), 134 (100), 121 (25).
11. Crystal data of (+)-5: orthorhombic (Equation)
12. We are grateful to NSC, R.O.C. for financial support of this research under Grant NSC 84-2331-B002-256 and NSC 85-2331-B-002-245.