A novel multiply primed polymerase chain reaction assay for identification of variant papG genes encoding the Gal(α1-4)Gal-binding PapG adhesins of Escherichia coli

Journal of Infectious Diseases

Volumn 173, Issue 4, 1996, Pages 920-926

  Johnson, James R ^a,b   Brown, Jennifer J ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIV OF MINNESOTA HOSP AND CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN;

ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI; GENE LOCATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0029889641     PISSN: 00221899     EISSN: None     Source Type: Journal    
DOI: 10.1093/infdis/173.4.920     Document Type: Article

References (8)

1. Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev 1991;4:80-128.
2. Hoschutzky H, Lottspeich F, Jann K. Isolation and characterization of the α-galactosyl-1,4-β-galactosyl-specific adhesin (P adhesin) from fimbriated Escherichia coli. Infect Immun 1989;57:76-81.
3. Lund B, Lindberg F, Marklund BI, Normark S. The papG protein is the α-D-galactopyranosyl-(1-4)-β-D-galactopyranose-binding adhesin of uropathogenic Escherichia coli. Proc Natl Acad Sci USA 1987;84:5898-902.
4. Marklund BI, Tennent JM, Garcia E, et al. Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific adhesive properties. Mol Microbiol 1992;6:2225-42.
5. Strömberg M, Marklund BI, Lund B, et al. Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Galα1-4Gal-containing isoreceptors. EMBO J 1990;9:2001-10.
6. Klann AG, Hull RA, Palzkill T, Hull SI. Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili. J Bacteriol 1994;176:2312-7.
7. Senior D, Baker N, Cedergren B, et al. Globo-A - a new receptor specificity for attaching Escherichia coli. FEBS Lett 1988;237:123-7.
8. Lund B, Marklund BI, Strömberg N, Lindberg F, Karlsson KA, Normark S. Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities. Mol Microbiol 1988;2:255-63.