Establishing a pure lymphocyte gate for subset analysis by flow cytometry

Communications in Clinical Cytometry

Volumn 26, Issue 2, 1996, Pages 172-177

  Horvatinovich, Joseph M ^a   Sparks, Sara D ^a   Mann, Karen P ^a  

^a DUKE UNIVERSITY   (United States)

Author keywords

Antigen specificity; Fluorescence intensity; Lymphocyte subset analysis; Monoclonal antibody; three color immunophenotyping; Whole blood

Indexed keywords

ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; FLOW CYTOMETRY; HELPER CELL; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LYMPHOCYTE SUBPOPULATION; NATURAL KILLER CELL; PRIORITY JOURNAL; SUPPRESSOR CELL; T LYMPHOCYTE;

ANTIGENS, CD; FLOW CYTOMETRY; HUMANS; LYMPHOCYTE SUBSETS;

EID: 0029888906     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19960615)26:2<172::AID-CYTO12>3.0.CO;2-K     Document Type: Article

References (6)

1. Carayon P, Bord A, Raymond M: Simultaneous identification of eight leukocyte subsets of human peripheral blood using three-colour immunofluorescence flow cytometric analysis. J Immunol Methods 138:257-264, 1991.
2. Centers for Disease Control: 1994 revised guidelines for the performance of CD4+ T-cell determinations in persons with human immunodeficiency virus (HIV) infection. Morbid Mortal Wkly Rep 43 (RR-3):1-21, 1994.
3. Givan AL: Flow Cytometry: First Principles. John Wiley & Sons, Inc., New York, 1992.
4. Horan PK, Slezak SE, Poste G: Improved flow cytometric analysis of leukocyte subsets: simultaneous identification of five cell subsets using two-color immunofluorescence. Proc Natl Acad Sci USA 83: 8561-8365, 1986.
5. Loken MR, Brosnan JM, Bach BA, Ault KA: Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry 11:453-459, 1990.
6. Nicholson JK, Jones BM, Hubbard M: CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry 14:685-689, 1993.