Tetrameric malate dehydrogenase from a thermophilic Bacillus: Cloning, sequence and overexpression of the gene encoding the enzyme and isolation and characterization of the recombinant enzyme

Biochemical Journal

Volumn 317, Issue 1, 1996, Pages 235-245

  Wynne, Samantha A ^a,b   Nicholls, David J ^b   Scawen, Michael D ^b   Sundaram, Trichur K ^a,c  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

^c NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; LACTATE DEHYDROGENASE; MALATE DEHYDROGENASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; ISOELECTRIC POINT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PLASMID; PRIORITY JOURNAL; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029888323     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3170235     Document Type: Article

References (54)

1. Sundaram, T. K., Wright, I. P. and Wilkinson, A. E. (1980) Biochemistry 19, 2017-2022
2. Smith, K., Sundaram, T. K. and Kernick, M. (1984). J. Bacteriol. 157, 684-687
3. Vogel, R. F., Entain, K. D. and Mecke, D. (1987) Arch. Microbiol. 149, 36-42
4. Nicholls, D. J., Minton, N. P., Atkinson, T. and Sundaram, T. K. (1989) Appl. Microbiol. Biotechnol. 31, 376-382
5. Nishiyama, M., Matsubara, N., Yamamoto, K., Iijima, S., Uozumi, T. and Beppu, T. (1986) J. Biol. Chem. 261, 14178-14183
6. Nicholls, D. J., Sundaram, T. K., Atkinson, T. and Minton, N. P. (1990) FEMS Microbiol. Lett. 70, 7-14.
7. Gleason, W. B., Fu, Z., Birktoft, J. and Banaszak, L. (1994) Biochemistry 33, 2078-2088
8. Birktoft, J. J., Bradshaw, R. A. and Banaszak, L. J. (1987) Biochemistry 26, 2722-2734
9. Hall, M. D., Levitt, D. G. and Banaszak, L. J. (1992) J. Mol. Biol. 226, 867-682
10. Kelly, C. A., Nishiyama, M., Ohnishi, Y., Beppu, T. and Birktoft, J. J. (1993) Biochemistry 32, 3913-3922
11. Smith, K. and Sundaram, T. K. (1988) Biochim. Biophys. Acta 955, 203-213
12. Banaszak, L. J. and Bradshaw, R. A. (1975) in The Enzymes (Boyer, P. D., ed.), pp. 363-396, Academic Press, New York
13. Holbrook, J. J., Liljas, A., Steindel, S. J. and Rossmann, M. G. (1975) in The Enzymes (Boyer, P. D., ed.), pp. 191-292, Academic Press, New York
14. McAlister-Henn, L. (1988) Trends Biochem. Sci. 13, 178-181
15. Grau, U. M., Trommer, W. E. and Rossmann, M. G. (1981) J. Mol. Biol. 151, 289-307
16. Clark, A. R., Atkinson, T., Campbell, J. W. and Holbrook, J. J. (1985) Biochim. Biophys. Acta 829, 387-396
17. Epstein, I. and Grossowicz, N. (1969) J. Bacteriol. 99, 414-417
18. Sundaram, T. K., Cazzulo, J. J. and Kornberg, H. L. (1969) Biochim. Biophys. Acta 192, 355-357
19. Chambers, S. P., Prior, S. E., Barstow, D. A. and Minton, N. P. (1988) Gene 68, 139-149
20. Brehm, J. K., Chambers, S. P., Brown, K. J., Atkinson, T. and Minton, N. P. (1991) Appl. Microbiol. Biotechnol. 36, 358-363
21. Messing, J. and Vieira, J. (1982) Gene 19, 269-276
22. Cohen, S. N., Chang, A. C. and Hsu, L. (1972) Proc. Natl. Acad. Sci. U.S.A. 69, 2110-2114
23. Marmur, J. (1961) J. Mol. Biol. 3, 208-218
24. Smith, K., Sundaram, T. K., Kernick, M. and Wilkinson, A. E. (1982) Biochim. Biophys. Acta 708, 17-25
25. Sanger, F., Nicklen, S. and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467
26. Gornall, A. G., Baradawill, C. J. and David, M. M. (1949) J. Biol. Chem. 177, 751-766
27. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
28. Sambrook, T., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
29. Staniforth, R. A., Burston, S. G., Smith, C. J., Jackson, G. S., Badcoe, I. G., Atkinson, T., Holbrook, J. J. and Clarke, A. R. (1993) Biochemistry 32, 3842-3851
30. Leatherbarrow, R. J. (1987) A Non-linear Regression Data Analysis Program for the IBM PC, Elsevier BIOSOFT, Cambridge
31. Alldread, R. M., Nicholls, D. J., Sundaram, T. K., Scawen, M. D. and Atkinson, T. (1992) Gene 114, 139-143
32. Sharp, R. J., Brown, K. J. and Atkinson, A. (1980) J Gen. Microbiol. 117, 201-210
33. Shine, J. and Dalgarno, L. (1974) Proc. Natl. Acad. Sci. U.S.A. 71, 1342-1346
34. Vellanoweth, R. L. (1993) in Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (Sonenshein, A. L., Hoch, J. A. and Losick, R., eds.), pp. 699-711, American Society for Microbiology, Washington, D.C.
35. Devereux, J., Haeberli, P. and Smithies, O. (1984), Nucleic Acids Res. 12, 387-395
36. Schwartz, R. M. and Dayhoff, M. O. (1979) in Atlas of Protein Sequences and Structure 5, Suppl. 3 (Dayhoff, M. O., ed.)., pp. 353-358, National Biomedical Research Foundation, Washington, D.C.
37. Risler, J. L., Delorme, M. O., Delacroix, H. and Henaut, A. (1988) J. Mol. Biol. 204, 1019-1029
38. Birktoft, J. J., Fernley, R. T., Bradshaw, R. A. and Banaszak, L. J. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 6166-6170
39. Eventoff, W., Rossmann, M. G., Taylor, S. S., Torff, H. J., Meyer, H., Keil, W. and Kiltz, H. H. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 2677-2681
40. Birktoft, J. J. and Banaszak, L. J. (1984) Pept. Protein Rev. 4, 1-46
41. Alldread, R. M., Halsall, D. M., Clarke, A. R., Sundaram, T. K., Atkinson, T., Scawen, M. D. and Nicholls, D. J. (1995) Biochem. J. 305, 539-548
42. Clarke, A. R., Atkinson, T. and Holbrook, J. J. (1989) Trends Biochem. Sci. 14, 101-105
43. Clarke, A. R., Smith, C. R., Hart, K. W., Wilks, H. M., Chia, W. N., Lee, T. J., Birktoft, J. L., Banaszak, L. J., Barstow, D. A., Atkinson, T. and Holbrook, J. J. (1987) Biochem. Biophys. Res. Commun. 148, 15-23
44. Nicholls, D. J., Miller, J., Scawen, M. D., Clarke, A. R., Holbrook, J. J., Atkinson, T. and Goward, C. R. (1992) Biochem. Biophys. Res. Commun. 189, 1057-1062
45. Clarke, A. R., Wigley, D. B., Barstow, D. A., Chia, W. N., Atkinson, T. and Holbrook, J. J. (1987) Biochim. Biophys. Acta 913, 72-80
46. Ben-Bassat, A. and Bauer, K. (1987) Nature (London) 326, 315
47. Duffieid, M. L., Nicholls, D. J., Atkinson, T. and Scawen, M. D. (1994) J. Mol. Graphics 12, 14-21
48. Goward, C. R., Miller, J., Nicholls, D. J., Irons, L. I., Scawen, M. D., O'Brian, R. and Chowdhry, B. Z. (1994) Eur. J. Biochem. 224, 249-255
49. Jin, S. and Sonenshein, A. L. (1994) J. Bacteriol. 176, 4669-4679
50. Anagostopoulos, C., Piggot, P. J. and Hoch, J. A. (1993) in Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (Sonenshein, A. L., Hoch, J. A. and Losick, R., eds.), pp. 425-461, American Society for Microbiology, Washington, D.C.
51. Bachmann, B. J. (1990) Microbiol. Rev. 54, 130-197
52. Jackson, R. M., Gelpi, J. L., Cortes, A., Emery, D. L., Wilks, H. M., Moreton, K. M., Halsall, D. J., Sleigh, R. N., Behanmartin, M., Jones, G. R., Clarke, A. R. and Holbrook, J. J. (1992) Biochemistry 31, 8307-8314
53. Cameron, A. D., Roper, D. I., Moreton, K. M., Muirhead, H., Holbrook, J. J. and Wigley, D. B. (1994) J. Mol. Biol. 238, 615-625
54. White, D., Sharp, R. J. and Priest, F. G. (1993) Antonie van Leeuwenhoek J. Microbiol. 64, 357-386