Technical problems arising from the use of the immunoblot for determination of the reactivity of natural antibodies with different lipopolysaccharides (LPS)

Journal of Immunological Methods

Volumn 190, Issue 2, 1996, Pages 185-188

  Schoenherr, G ^a   Roggenbuck, D ^b,d   Seifert, M ^b   Jahn, S ^b   Porstmann, T ^b,c  

^a Helios Hospital Berlin Buch   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c Seramun Diagnostica GmbH   (Germany)

^d Dr Fooke Laboratorien GmbH   (Germany)

Author keywords

Immunoblot; Lipopolysaccharide; Polyreactive antibody

Indexed keywords

LIPOPOLYSACCHARIDE; MONOCLONAL ANTIBODY; POLYVINYLIDENE DIFLUORIDE; PYROXYLIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HOST RESISTANCE; HUMAN; IMMUNOBLOTTING; PRIORITY JOURNAL;

NEGIBACTERIA;

EID: 0029887861     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00271-5     Document Type: Article

References (10)

1. Avrameas, S. and Ternynck, T. (1993) The natural autoantibodies system: between hypothesis and facts. Mol. Immunol. 30, 1133-1142.
2. Di Padova, F.E., Brade, H., Barclay, G.R., Poxton, I.R., Liehl, E., Schuetze, E., Kocher, H.P., Ramsay, G., Schreier, M.H., McClelland, D.B.L. and Rietschel, E.T. (1993) A broadly cross-protective monoclonal antibody binding to Escherichia coli and Salmonella lipopolysaccharides. Infect. Immun. 61, 3863-3872.
3. Gu, X.-X. and Tsai, C.-M. (1991) Purification of rough-type lipopolysaccharides of Neisseria meningitidis from cells and outer membrane vesicles in spent media. Anal. Biochem. 196, 311-318.
4. Jahn, S., Niemann, B., Winkler, T., Kalden, J.R., von Baehr, R. (1993) Expansion of a B-lymphocyte clone producing IgM autoantibodies encoded by a somatically mutated Vh1 gene in the spleen of an autoimmune patient. Rheumatol. Int. 13, 583-593.
5. Kuhn, H.-M. (1993) Cross reactivity of monoclonal antibodies and sera directed against Lipid A and lipopolysaccharides. Infection 21, 179-186.
6. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
7. Lynn, W.A. and Golenbock, D.T. (1992) Lipopolysaccharide antagonists. Immunol. Today 13, 271-276.
8. Pollack, M., Raubitschek, A.A. and Larrick, J.W. (1987) Human monoclonal antibodies that recognize conserved epitopes in the core-lipid A region of lipopolysaccharides. J. Clin. Invest. 79, 1421-1430.
9. Roggenbuck, D., Marx, U., Kiessig, S.T., Jahn, S., Schoenherr, G. and Porstmann, T. (1994) Purification and immunochemical characterization of a natural polyreactive monoclonal IgM antibody. J. Immunol. Methods 167, 207-218.
10. Tsai, C.-M. (1986) The analysis of lipopolysaccharide (endotoxin) in meningococcal polysaccharide vaccines by silver staining following SDS-polyacrylamide gel electrophoresis. J. Biol. Standard. 14, 25-33.